Biological, Serological and Molecular Characterization of Egyptian Zucchini Yellow Mosaic Virus Isolate Infecting Squash Plants in Fayoum Governorate

Kheder, A; Sulaiman, Taqwa; Ghanem, G. A. M.; Tohamy, M.R.A.

doi:10.21608/ejp.2017.88570

Cited by 5 publications

(8 citation statements)

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“…Significant yield losses due to ZYMV infections have been reported ranging from 20 to 78% in Egypt (Kheder et al 2017); 50 to 94% or up to 80% in Western Australia (Coutts et al 2011) and in Germany (Müller et al 2006), respectively; and 19.7 to 84.9% in the Ivory Coast (Kone et al 2017). Symptoms characteristic of a ZYMV infection were clearly detected in the surveyed fields (Figure 1).…”

Section: Discussionmentioning

confidence: 96%

“…The information about the sequence diversity among the isolates may help design primers for the reliable detection of all the known virus isolates. Earlier studies on the virus in Egypt (El-Hoseny et al 2010;Kheder et al 2017) were based on the RT-PCR detection and the genetic diversity of the virus in Egypt was lacking. Based on the CP phylogenetic analyses from previous worldwide studies, the ZYMV isolates have been classified into two or three major groups (Coutts et al 2011;Massumi et al 2011;Maina et al 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have indicated the ZYMV infections of cucurbits in Egypt (Nasr-Eldin et al 2016;Kheder et al 2017). Reverse transcription polymerase chain reaction (RT-PCR) has been used to detect the virus coat protein (CP) gene or other genome segments.…”

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confidence: 99%

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Molecular characterisation of Zucchini yellow mosaic virus infecting Cucurbita pepo in Egypt

Aleem¹,

Rabie²,

Fattouh³

2021

Plant Prot. Sci.

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The complete nucleotide sequence of Zucchini yellow mosaic virus isolate from Egypt (ZYMV-Egz_MT383108) was determined. The sequence comparisons suggested that the isolate belongs to Group A. The sequence analysis of the Egyptian isolate showed the highest similarity (~96–97%) with the isolates leaf1 (KJ923767.1) and PA_2006 (JQ716413.1) from the USA and the lowest similarity (84%) with an isolate (AF014811.2) from Singapore. The phylogenetic analysis revealed that ZYMV-Egz occupied a distinct clade together with the USA isolates in Group A, known to be the most widespread throughout the world. This is a first record of the complete nucleotide sequence of an Egyptian isolate of ZYMV.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

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Molecular characterisation of Zucchini yellow mosaic virus infecting Cucurbita pepo in Egypt

Aleem¹,

Rabie²,

Fattouh³

2021

Plant Prot. Sci.

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“…Most of the studies have been performed serologically, biologically and symptomatologically, but molecular characterization for its coat protein gene is very limited, besides no genomic sequence information was reported except for 2-3 studies. ZYMV can be detected with different primer sets, referred on coat protein (CP) ve NIb gene (Karamanlı and Kamberoğlu, 2010;Wang and Li, 2017;Kheder et al, 2017;Nasr-Eldin et al, 2016;Prieto et al, 2001). We identified the ZYMV using ZS and ZA primer pairs designed specifically for the CP gene.…”

Section: B Cmentioning

confidence: 99%

“…ZYMV is a worldwide plant pathogenic virus that parasitizes cucurbit plants including Cucurbita pepo, C. pepo, C. moschata, C. maxima, Cucumis sativus, Cucumis melo, Citrullus lanatus, and can causes substantially product losses up to 94% every year in all seasons except winter (Wang et al, 1992). It has also been reported that some weeds and cultivated plants such as Ranunculus sardous, Lamium amplexicaule, Sesamum indicum, Ranunculus sardous, Chenopodium amaranticolour, C. quinoa, Phaseolus vulgaris, Nicotiana benthamiana, Cucurbita pepo, and Cucumis melo are the experimental hosts, Melothria pendula and Moluccella laevis, which are wild cucurbit species are natural hosts of ZYMV, can also serve as inoculum resources in the pathogen life table (Mahgoub et al, 1997;Kheder et al, 2017;Adlerz et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Occurrence and Characterization of Coat Protein Gene of Zucchini yellow mosaic potyvirus (ZYMV) Isolate Infecting Pumpkin (Cucurbita pepo L.) in Bingol Province (Turkey)

Güller¹,

Usta²

2019

Türk Tarım Ve Doğa Bilimleri Dergisi

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Zucchini yellow mosaic potyvirus (ZYMV) belongs to family Potyviridae, which causes serious economic losses in many cucurbits from worldwide. In 2018 (September), leaf samples of pumpkin exhibiting typical viral symptoms including mosaic, blistering and wrinkling and healthy pumpkin leaf samples were collected from Bingöl and screened by Reverse-Transcription Polymerase Chain Reaction (RT-PCR) against ZYMV infection. Tested leaf samples were reacted positive resulting in an expected about 840 bp DNA fragments of partial coat protein (CP) gene of ZYMV. ZYMV-CP gene was further inserted into a pGEM-T Easy prokaryotic cloning vector and their partial nucleotide sequences and deduced amino acid were ascertained. The provided ZYMV-CP gene sequence consisted of 837 nucleotides in length coded for 279 amino acid residues of approximately 31.2 kDa. The isolate was denominated as ZYMV-Bingol and registered with MK689858 accession number in the NCBI. The sequence of ZYMV-Bingol CP gene were aligned with 21 isolates deposited in GenBank from different geographical location and its phylogenetic relationships were determined. Molecular analysis of the ZYMV CP gene sequence indicated the highest similarity with 100% Turkish isolate (JF317296) and the lowest with 91.64% of Korea isolate (AF062518), at nucleotide level. Moreover, phylogenetic analyses revealed that ZYMV Bingöl isolate is clustered with the Turkish-Adana isolate (JF317296) and Pakistan isolate (AB127936). ZYMV has been reported for the first time in the pumpkin plant from Bingöl province of Turkey by this study.

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Viral disease control in strawberry plants: A potential impact of green nanotechnology

Shafie¹,

Abdelkader²

2023

MJM

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Aims: Strawberry (Fragaria ananassa Duch.) is a member of the family Rosaceae and one of the most important vegetable crops in Egypt for local fresh consumption and exportation. Suspected symptoms of Strawberry mild yellow edge virus (SMYEV) on naturally infected strawberry plants were observed in Al Qaluobia Governorate. The main goal of this study was to develop a novel eco-friendly biogenic silver nanoparticle using clove (Syzygium aromaticum) (Sz-AgNPs) aqueous extract to control viral infection. Methodology and results: Mechanically inoculated sixteen plant species representing seven families, Apocynaceae, Chenopodiaceae, Cucurbitaceae, Fabaceae, Moraceae, Rosaceae and Solanaceae, were used as hosts for SMYEV. Systemic mosaic, chlorotic local lesions, vein clearing, marginal chlorosis, marginal necrosis, yellowing and vein banding were observed 7 to 20 days post-inoculation. Transmission electron microscopy (TEM) of diseased tissues revealed severe degeneration of the chloroplasts and nucleus structures and the formation of cell wall protrusions. Sz-AgNPs were characterized using UV-Vis spectroscopy, TEM, dynamic light scattering (DLS) and Fourier transform infrared (FTIR). Foliar application of Sz-AgNPs (200 µg/mL) 24 h post and/or concurrently with SMYEV inoculation dropped the infection rate by 90% and 55%, respectively, whereas it was only reduced by 35% when applied 24 h prior to viral inoculation compared to control groups. DAS-ELISA and RT-PCR verified SMYEV inhibition (75-90%) in all plants treated with 200, 150 and 100 µg/mL Sz-AgNPs 24 h post-viral inoculation. Complete viral eradication was attained by applying Sz-AgNPs at a concentration of 200 μg/mL 24 h post-virus inoculation. Moreover, the total phenols, indoles, Vitamin C, total flavonoids and citric acid contents were not significantly affected by Sz-AgNPs treatment compared to healthy control groups. Conclusion, significance and impact of study:In conclusion, Sz-AgNPs suppressed SMYEV by 75-90% when applied 24 h post-virus inoculation. Eco-friendly Sz-AgNPs could be used for controlling viral infections and avoiding the rejection of exportable strawberries due to the use of harmful pesticides.

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Biological, Serological and Molecular Characterization of Egyptian Zucchini Yellow Mosaic Virus Isolate Infecting Squash Plants in Fayoum Governorate

Cited by 5 publications

References 0 publications

Molecular characterisation of Zucchini yellow mosaic virus infecting Cucurbita pepo in Egypt

Molecular characterisation of Zucchini yellow mosaic virus infecting Cucurbita pepo in Egypt

Occurrence and Characterization of Coat Protein Gene of Zucchini yellow mosaic potyvirus (ZYMV) Isolate Infecting Pumpkin (Cucurbita pepo L.) in Bingol Province (Turkey)

Viral disease control in strawberry plants: A potential impact of green nanotechnology

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