Biological Properties of a Renotropic Protein Present in Plasma of Uninephrectomized Rats

Esbrit, Pedro; Garcı́a-Ocaña, Adolfo; García-Cañero, R.; Manzano, F.; Jiménez-Clavero, M.A.

doi:10.1159/000173410

Cited by 6 publications

(6 citation statements)

References 23 publications

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“…Specifically, N-terminal peptides and, in particular, y-melanocyte-stimulating hormone have been shown to increase post-nephrectomy and stimulate natriuresis via a distal tubular effect (Qiu, Valentin, Chen, Wiedeman & Humphreys, 1992 1985), and the present experiments suggest that this occurs in the proximal tubule and loop of Henle and may be inducible by the presence of a transferable factor. In the porcine-derived immortalized proximal tubular cell line (LLCPK1) the addition of serum from nephrectomized animals has been shown to stimulate Na+ transport (Esbrit et al 1991). Enhanced proximal tubular cell growth has been observed in association with stimulation of apical Na+-H+ exchange and hence secondary stimulation of basolateral Na+-K+-ATPase (Fine et al 1985).…”

Section: Resultsmentioning

confidence: 99%

Transferable circulating factors and epithelial sodium transport after unilateral nephrectomy in the rat.

Pollock

Nobes

Györy

et al. 1996

The Journal of Physiology

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1. In order to investigate the role of circulating serum factors in the altered renal haemo-dynamics and enhanced renal tubular transport observed in renal growth, micropuncture experiments were performed on normal animals infused with 20% plasma derived from animals in whom unilateral nephrectomy had been performed 3 days previously. 2. When animals infused with plasma from uninephrectomized animals (NxP) were compared with those infused with control plasma, the former had a higher tubular fluid flow rate measured at both the late proximal (LP; 26-7 + 1P6 vs. 18i4 + 1P4nl min-'; P< 0-001) and early distal (ED; 14 9 + P 0 vs. 4. Tubular Nae transport was higher in the animals infused with NxP as evidenced by a decrease in the fractional delivery of Nae at the ED site (4 5 + 0A4 vs. 6-5 + 0-6% of the filtered load; P< 0 05). However, in the final urine there was a significant increase in the urinary sodium excretion in animals infused with NxP (0-67 + 0-14 vs. 0-29 + 0 09%; P < 0 05) indicating that natriuresis and probably diuresis was a result of inhibition of Nae and water transport in the late distal tubule and collecting duct. 5. In conclusion, these experiments demonstrate that circulating factors induced by a reduction in renal mass significantly alter glomerular filtration and tubular Nae transport.

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Section: Resultsmentioning

confidence: 99%

Transferable circulating factors and epithelial sodium transport after unilateral nephrectomy in the rat.

Pollock

Nobes

Györy

et al. 1996

The Journal of Physiology

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“…Measurement o f D N A synthesis in rat cortical tubules was carried out as described previously [9]. Briefly, 0.…”

Section: Assays O F Dna Synthesismentioning

confidence: 99%

“…Thus, hyperplasia has been shown to be predominant in young subjects as well as after extensive nephron loss [3,4], Cur rent evidence indicates that regulation of renal compensat ory growth involves at least in part a humoral mechanism [5][6][7]. Recently, we have partially purified from uninephrec tomized rat plasma a renal growth factor activity with some properties different from these of other known renotropic factors [8][9][10], In the present studies, a renotropic activity from plasma of human kidney transplantation donors and a renal cancer patient after uninephrectomy (UNX) has been …”

Section: Introductionmentioning

confidence: 99%

Partial Purification of a Renotropic Activity from Plasma of Uninephrectomized Human Subjects

Garcı́a-Ocaña

Ortega²,

González‐García³

et al. 1993

Nephron

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A renotropic factor was partially purified by sequential gel filtration and anion exchanger chromatography from plasma of human kidney transplantation donors and a renal cancer patient after uninephrectomy. This activity increased the rate of [³H]thymidine incorporation into DNA in rat cortical tubules, but not in rat liver cells, within the range of 100-200 ng/ml protein. The renotropic activity was detected between 7 and 12 days after uninephrectomy, and at least in 1 case decreased thereafter. This activity was undetected in gel-filtrated plasma of patients after a nonurological surgical procedure. The potency of this renotropic activity and its elution by gel filtration are similar to those displayed by a renal growth factor activity isolated from uninephrectomized rat plasma, as recently reported.

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“…the heart and blood vessels) (Banfic & Kukolja 1988;Esbrit et al 1991;Ferrerira et al 1995;Reams et al 1995;Pollock et al 1996;Valentin 1997). After nephrectomy or after partial renal dysfunction or damage, the remaining renal mass undergoes some functional changes, yet unknown in detail (Griffin et al 1992;Bidani et al 1994;Huang et al 1994), to be able to carry out the normal function and blood pressure control.…”

mentioning

confidence: 99%

Beneficial Effects of Trandolapril in Uninephrectomized Spontaneously Hypertensive Rats: Role of Cyclooxygenase Pathway

López‐Hernández

Carrón

Montero

2002

Pharmacology & Toxicology

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Abstract:The antihypertensive efficacy of the angiotensin-converting enzyme inhibitor trandolapril was evaluated in uninephrectomized spontaneously hypertensive rats. After 5 weeks of treatment, blood pressure, cardiac and aortic mass, as well as the functional status of the aortic endothelium, and the role played by the cyclooxygenase pathway were investigated. In addition, the effect of a sub-antihypertensive dose of the calcium antagonist verapamil, in combination with trandolapril, was also investigated. As compared to placebo, trandolapril returned blood pressure and aortic lamina media cross sectional area to normotensive values, significantly reduced the heart-to-body weight, and improved the acetylcholine-induced relaxation of aortic rings. This latter effect is thought to be mediated by the elimination of a substance derived from the cyclooxygenase pathway. Verapamil, in single therapy, did not reduce blood pressure, or heart-to-body weight, increased aortic lamina media cross sectional area and impaired acetylcholine-induced relaxation. When combined with trandolapril in dual therapy, some of the beneficial effects of trandolapril remained, whereas others were counterbalanced by verapamil. In conclusion, trandolapril proved to be an effective therapeutic drug in this animal model of hypertension. Combination of trandolapril with a sub-antihypertensive dose of verapamil did not show any positive synergistic effect; on the contrary, it outweighed some of the beneficial effects of trandolapril.

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Biological Properties of a Renotropic Protein Present in Plasma of Uninephrectomized Rats

Cited by 6 publications

References 23 publications

Transferable circulating factors and epithelial sodium transport after unilateral nephrectomy in the rat.

Transferable circulating factors and epithelial sodium transport after unilateral nephrectomy in the rat.

Partial Purification of a Renotropic Activity from Plasma of Uninephrectomized Human Subjects

Beneficial Effects of Trandolapril in Uninephrectomized Spontaneously Hypertensive Rats: Role of Cyclooxygenase Pathway

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