Biological nanopores elucidate the differences between isomers of mercaptobenzoic-capped gold clusters

Cox, Bobby D.; Martin, Charles R.; Bertino, Massimo F.; Reiner, Joseph E.

doi:10.1039/d0cp05671e

Cited by 4 publications

(2 citation statements)

References 36 publications

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“…Nanopore analysis can be used in conjunction with microcapillary tips to load individual metallic clusters into a pore. , This has been used to study ligand exchange processes on metallic clusters, and we use this methodology here to further explore peptide induced current fluctuations. Figure illustrates the experimental setup and typical traces that demonstrate peptide attachment.…”

Section: Resultsmentioning

confidence: 99%

Selective Detection and Characterization of Small Cysteine-Containing Peptides with Cluster-Modified Nanopore Sensing

et al. 2022

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It was recently demonstrated that one can monitor ligand-induced structure fluctuations of individual thiolate-capped gold nanoclusters using resistive-pulse nanopore sensing. The magnitude of the fluctuations scales with the size of the capping ligand, and it was later shown one can observe ligand exchange in this nanopore setup. We expand on these results by exploring the different types of current fluctuations associated with peptide ligands attaching to tiopronin-capped gold nanoclusters. We show here that the fluctuations can be used to identify the attaching peptide through either the magnitude of the peptide-induced current jumps or the onset of high-frequency current fluctuations. Importantly, the peptide attachment process requires that the peptide contains a cysteine residue. This suggests that nanopore-based monitoring of peptide attachments with thiolate-capped clusters could provide a means for selective detection of cysteine-containing peptides. Finally, we demonstrate the cluster-based protocol with various peptide mixtures to show that one can identify more than one cysteine-containing peptide in a mixture.

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Section: Resultsmentioning

confidence: 99%

Selective Detection and Characterization of Small Cysteine-Containing Peptides with Cluster-Modified Nanopore Sensing

et al. 2022

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“…However, due to a lack of a sensing method with a sufficiently high spatial and temporal resolution, direct observation of this switching behavior has not been reported previously. Though nanopores have been previously applied in the discrimination of a wide variety of chiral isomeric analytes, ,− this approach however differs from those by monitoring different chiral configurations of the same analyte molecule when bound to the reactive site of the pore.…”

Section: Introductionmentioning

confidence: 99%

Single-Molecule Interconversion between Chiral Configurations of Boronate Esters Observed in a Nanoreactor

Zhang

Wang

et al. 2023

ACS Nano

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Isomers of some chemical compounds may be dynamically interconvertible. Due to a lack of sensing methods with a sufficient resolution, however, direct monitoring of such processes can be difficult. Engineered Mycobacterium smegmatis porin A (MspA) nanopores can be applied as nanoreactors so that chemical reactions can be directly monitored. Here, an MspA modified with a phenylboronic acid (PBA) adapter was prepared and was used to observe dynamic interconversion between chiral configurations of boronate esters, which appears as telegraphic switching on top of nanopore events. The mechanism of this behavior was further confirmed by trials with different halogenated catechols, dopamine, adenosine, 1,2-propanediol, and (2R,3R)-2,3-butanediol, and its generality has been demonstrated. These results suggest that an engineered MspA possesses an exceptional resolution in its monitoring of chemical reaction processes and may inspire the future design of nanopore small-molecule sensors.

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Structure-activity relationship between gold nanoclusters and human serum albumin: Effects of ligand isomerization

Kang,

Xue,

Miao

et al. 2024

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Biological nanopores elucidate the differences between isomers of mercaptobenzoic-capped gold clusters

Abstract: Nanopore sensing can be applied to analyze ligand-induced structural fluctuations of metallic nanoclusters. This analysis is applied here to demonstrate various isomer effects for mercaptobenzoic-capped gold nanoclusters.

Cited by 4 publications

References 36 publications

Selective Detection and Characterization of Small Cysteine-Containing Peptides with Cluster-Modified Nanopore Sensing

Selective Detection and Characterization of Small Cysteine-Containing Peptides with Cluster-Modified Nanopore Sensing

Single-Molecule Interconversion between Chiral Configurations of Boronate Esters Observed in a Nanoreactor

Structure-activity relationship between gold nanoclusters and human serum albumin: Effects of ligand isomerization

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