Biological insect control using Metarhizium anisopliae: morphological, molecular, and ecological aspects

Tiago, Patrícia Vieira; Oliveira, Neiva Tinti de; Lima, Elza Áurea de Luna Alves

doi:10.1590/s0103-84782014000400012

Cited by 24 publications

(19 citation statements)

References 32 publications

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“…No similarities between these fungi were found: they belong to different order, Pleosporales and Hypocreales. Alternaria are ubiquitous filamentous fungi, which are found in soil air and human/animal skin (Pastor and Guarro 2008); Metarhizium is commonly found as a parasite of insects and symbiont of plants (Samish et al 2014;Tiago and Oliveira 2014).…”

Section: Identification Of Microbiological Cultures and Mock Communitmentioning

confidence: 99%

Rapid and real-time identification of fungi up to the species level with long amplicon Nanopore sequencing from clinical samples

D’Andreano

Cuscó

Francino

2020

Preprint

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ABSTRACTThe availability of long-read technologies, like Oxford Nanopore Technologies, provides the opportunity to sequence longer fragments of the fungal ribosomal operon, up to 6 Kb (18S-ITS1-5.8S-ITS2-28S), and to improve the taxonomy assignment of the communities up to the species level and in real-time. We assess the taxonomy skills of amplicons targeting a 3.5 Kb region (V3 18S-ITS1-5.8S-ITS2-28S D2) and a 6 Kb region (V1 18S-ITS1-5.8S-ITS2-28S D12) with the What’s in my pot (WIMP) classifier. We used the ZymoBIOMICS™ mock community and different microbiological fungal cultures as positive controls. Long amplicon sequencing correctly identified Saccharomyces cerevisiae and Cryptococcus neoformans from the mock community and Malassezia pachydermatis, Microsporum canis, and Aspergillus fumigatus from the microbiological cultures. Besides, we identified Rhodotorula graminis in a culture mislabeled as Candida spp.We applied the same approach to external otitis in dogs. Malassezia was the dominant fungal genus in dogs’ ear skin, whereas M. pachydermatis was the main species in the healthy sample. Conversely, we identified a higher representation of M. globosa and M. sympodialis in otitis affected samples. We demonstrate the suitability of long ribosomal amplicons to characterize the fungal community of complex samples, either healthy or with clinical signs of infection.

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Section: Identification Of Microbiological Cultures and Mock Communitmentioning

confidence: 99%

Rapid and real-time identification of fungi up to the species level with long amplicon Nanopore sequencing from clinical samples

D’Andreano

Cuscó

Francino

2020

Preprint

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“…Both Metarhizium and Beauveria act by degrading cuticle of the insect and produces mycolytic enzyme to control the pest (Hatting et al, 2004;Wang et al, 2004). Species belonging KEYWORDS: Bioassay, compatibility, entomopathogenic fungus, insecticide, mealybug (Article chronicle: Received: 25-11-2018;Revised: 12-08-2019;Accepted: 02-09-2019) to genus Metarhizium are well known to target large range of host insects (Tiago et al, 2014). Beauveria bassiana (Bals.)…”

Section: Introductionmentioning

confidence: 99%

Compatibility of insecticides with Metarhizium brunneum (Petch) and Beauveria bassiana(Bals.) for bio-intensive management of pink mealybug, Maconellicoccus hirsutus (Green)in grapes

Yadav

Ranade

Mhaske

et al. 2019

Journal of Biological Control

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Compatibility of insecticides with Metarhizium brunneum (Petch) and Beauveria bassiana (Bals.) for bio-intensive management of pink mealybug, Maconellicoccus hirsutus (Green) in grapes ABSTRACT: Grape (Vitis vinifera Linnaeus) is a high-value crop and important as a valuable export commodity for India. Pink mealybug, Maconellicoccus hirsutus (Green) is one of the most important pests infesting grapes. Two entomopathogenic fungi were isolated from the field infected insects and were identified as Metarhizium brunneum (Petch) and Beauveria bassiana (Bals.). The pathogenicity study showed that both the fungi were capable of infecting M. hirsutus. LC 50 values 1.4 × 10 6 and 1.0 × 10 7 conidia per ml was recorded for M. brunneum and B. bassiana, respectively. Evaluation of compatibility of these fungi with insecticides is important to develop bio-intensive management strategy for mealybugs. The compatibility of seven insecticides (emamectin benzoate, tolfenpyrad, imidacloprid, clothianidin, buprofezin, fipronil, spirotetramat) with these entomopathogens was evaluated under laboratory conditions. Compatibility studies based on sporulation, germination and vegetative growth of fungi showed that imidacloprid and emamectin benzoate were most compatible and tolfenpyrad and spirotetramat were highly incompatible with both the entomopathogens.

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“…H 2 O 2 has a recognized potential for causing oxidative stress in living organisms (Vännin & Koskula, 1998;Qin et al, 2011;Wojtyla et al, 2016) and is widely used as a disinfectant and sterilant (Watt et al, 2004). The evaluation of entomopathogenic fungi to replace synthetic insecticides, in the control of fungus gnats and shore fly, was also explored as a more sustainable form of fly control (Stanghellini & El-Hamalawi, 2005) and other vector insects (Tiago et al, 2014), in a hydroponic system.…”

Section: Introductionmentioning

confidence: 99%

Hydrogen Peroxide for Insect and Algae Control in a Lettuce Hydroponic Environment

Caixeta¹,

Mata²,

Curvêlo³

et al. 2018

JAS

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Insects and algae demonstrate adaptability in hydroponic environments. Algae attract flies, Bradysia spp. (Diptera: Sciaridae) and Scatella stagnalis (Diptera: Ephydridae), called fungus gnats and shore fly, respectively. Flies feed on algae, damaging seedlings radicellae, and may transmit pathogens to lettuce plants. Little information on the management of flies and algae is known. A paradox in the face of the expansion of hydroponics. The objective was to evaluate the potential of hydrogen peroxide (H 2 O 2 ) as an insecticide and algaecide agent. Entomopathogenic fungi were also evaluated as an alternative control to flies. The experiment was conducted in a commercial hydroponic system under a randomized complete block design with 5 treatments and 4 replicates. The treatments, sprayed on the phenolic foam plates, immediately after lettuce seed deposition, were: H 2 O 2 , Beauveria bassiana, Metarhizium anisopliae, Spinosyn and water, as control. The quality of the lettuce seedlings (cv. Brida), the presence of flies and algae evolution in the phenolic foam plates were recorded daily. H 2 O 2 and Spinosyn affected lettuce seed germination, but were able to reduce adult flies on the phenolic foam cells. The highest number of larvae was observed with both fungi, and only water, compared to H 2 O 2 . No larvae were found in Spinosyn sprayed phenolic foam cells. However, lettuce seedlings from Spinosyn sprayed plates were those with lower fresh weight. H 2 O 2 severely retained algae infestation in phenolic foam cells over a 15-day observation period. The present work demonstrates the potential that H 2 O 2 can play as an insecticidal and algaecide agent in hydroponic environments.

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Biological insect control using Metarhizium anisopliae: morphological, molecular, and ecological aspects

Abstract: Microbial control of insects is based on the rational use of pathogens to maintain environmentally balanced pest population levels, and

Cited by 24 publications

References 32 publications

Rapid and real-time identification of fungi up to the species level with long amplicon Nanopore sequencing from clinical samples

Rapid and real-time identification of fungi up to the species level with long amplicon Nanopore sequencing from clinical samples

Compatibility of insecticides with Metarhizium brunneum (Petch) and Beauveria bassiana(Bals.) for bio-intensive management of pink mealybug, Maconellicoccus hirsutus (Green)in grapes

Hydrogen Peroxide for Insect and Algae Control in a Lettuce Hydroponic Environment

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