Biological Influence of Deuterium on Procariotic and Eukaryotic Cells

Mosin, Oleg; Ignatov, Ignat

doi:10.13187/ejmb.2014.3.11

Cited by 16 publications

(16 citation statements)

References 23 publications

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“…The adapted B. methylicum eventually came back to normal growth at placing over in protonated growth medium after some lag-period that proves phenotypical nature of a phenomenon of adaptation that was observed for others adapted by us strains of methylotrophic bacteria and representatives of other taxonomic groups of microorganisms [19,20]. The effect of reversion of growth in protonated/deuterated growth media proves that adaptation to 2 Н2О is rather a phenotypical phenomenon, although it is not excluded that a certain genotype determines the manifestation of the same phenotypic trait in the growth media with different isotopic compositions.…”

Section: Facultative Methylotrophsmentioning

confidence: 67%

Evolution, Metabolism and Biotechnological Usage of Methylotrophic Microorganisms

Mosin¹,

Ignatov²

2014

European Journal of Molecular Biotechnology

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Methylotrophs -aerobic chemoheterotrophic microorganisms submitted by cocci and bacilli mobile forms, are inhabitants of reservoirs and soils of various type, where there are going on various processes of decomposition of organic substances with formation of the one-carbon С1-compounds and some С2-, and С3-compounds, capable to be assimilated by methylotrophs. These microorganisms assimilating carbon on ribuloso-5-monophospate and serine pathways, are allocated from soil ground, the sewage containing decomposing vegetative remainss, from ruminant paunch and other sources. Methylotrophic bacteria recently draw the increasing attention of biotechnology as feasible sources of natural biologically active compounds -fodder fibers and irreplaceable amino acids, carotenoid pigments, lipids and policarbohydrates. For preparation of these compounds are used genetically marked strains of methylotrophic bacteria, obtained via genetic engineering approaches and selection. Recently developed gene-engineering methods of manipulation with the methylotrophic genom allow create on the basis of microbic DNA of methylotrophs expression vectors of eukaryotic proteins for medical and veterinary purposes, as human insulins. In this review article there are submitted data including the results of the authors' own research on evolution of methylotrophic bacteria, the metabolism and their biotechnological usage.

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Section: Facultative Methylotrophsmentioning

confidence: 67%

Evolution, Metabolism and Biotechnological Usage of Methylotrophic Microorganisms

Mosin¹,

Ignatov²

2014

European Journal of Molecular Biotechnology

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“…This effect is known as kinetic isotopic effect of a solvent. The natural water consists on 99,7 mol.% of H2 16 O, which molecules are formed by 1 The average ratio of atoms of deuterium and hydrogen in natural waters compiles ~1:5700. In natural waters, the deuterium content is distributed irregularly: from 0,02-0,03 mol.% for river and sea water, to 0,015 mol.% for water of Antarctic ice -the most purified from deuterium natural water containing deuterium in 1,5 times less than that of seawater.…”

Section: Isotopic Effects Of Deuterium In Biological Objectsmentioning

confidence: 99%

“…10 -6 (89 ppm). Content of the lightest isotopologue -H2 16 The equilibrium vapor pressure of water isotopologues is differed quite significantly. The smaller the mass of the water molecule, the higher the vapor pressure, meaning that the vapor being in equilibrium with water, is always enriched with light isotopes of oxygen and hydrogen.…”

Section: Isotopic Effects Of Deuterium In Biological Objectsmentioning

confidence: 99%

Studying of Isotopic Effects of Deuterium in Biological Objects

Mosin¹,

Ignatov²

2015

European Reviews of Chemical Research

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This article describes the data on isotopic effects of deuterium in various biological objects as the cells of methylotrophic, chemoheterotrophic, photoorganotrophic microorganisms, green algae as well as animal cells. It was demonstrated that the increased content of deuterium in water leads to physiological, morphological and cytology alterations of the cell, and also renders negative influence on cellular metabolism, while deuterium depleted water (DDW) with decreased deuterium content on 20-30 % exerts beneficial effects on organism. The maximum kinetic isotopic effect measured at ordinary temperatures in chemical reactions leading to rupture of bonds involving hydrogen and deuterium lies in the range kH/kD = 6-8 for C-H versus C-D, N-D versus N-D, and O-H versus O-D-bonds. By IR-spectroscopy method water samples with varying content of deuterium were investigated.

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“…The traditional method for production of deuterium labelled compounds consists in the growth on media containing maximal concentrations of 2 H2O and deuterated substrates as [ 2 H]methanol, [ 2 H]glucose etc. [15,16]. During growth of cells on 2 H2O are synthesized molecules of biologically important natural compounds (DNA, proteins, amino acids, nucleosides, carbohydrates, fatty acids), which hydrogen atoms at the carbon backbones are completely substituted with deuterium.…”

Section: Introductionmentioning

confidence: 99%

Studying Physiological, Morphological, and Cytological Alterations in Prokaryotic and Eukaryotic Cells in Heavy Water

Mosin¹,

Ignatov²,

Складнев³

et al. 2015

Russian Journal of Biological Research

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In this paper the biological influence of deuterium on cells of various taxonomic groups of prokaryotic and eucaryotic microorganisms realizing methylotrophic, chemoheterotrophic, photoorganotrophic, and photosynthetic ways of assimilation of carbon substrates (methylotrophic bacteria Brevibacterium methylicum, chemoheterotrophic bacteria Bacillus subtilis, photoorganotrophic halobacteria Halobacterium halobium, and green algae Chlorella vulgaris) was investigated at the growth on media with heavy water ( 2 H2O). For all investigated microorganisms are submitted data on growth and adaptation on growth media containing as sources of deuterated substrates 2 H2O, [ 2 H]methanol and hydrolysates of deutero-biomass of methylotrophic bacteria B. methylicum, obtained after the multistage adaptation to 2 H2O. The qualitative and quantitative composition of intra-and endocellular amino acids, proteins, carbohydrates and fatty acids in conditions of adaptation to 2 H2O is investigated. It is shown, that the effects observed at adaptation to 2 H2O, possess a complex multifactorial character and connected to physiological, morphological 143Russian Journal of Biological Research, 2015, Vol. (5), Is. 3 and cytological alterations -the magnitude of the lag-period, time of cellular generation, output of biomass, a parity ratio of synthesized amino acids, proteins, carbohydrates and lipids, and also with an evolutionary level of the organization of the investigated object and the pathways of assimilation of carbon substrates.Keywords: deuterium, heavy water, adaptation, isotopic effects, bacteria, micro algae. IntroductionThe most interesting biological phenomenon is the ability of some microorganisms to grow on heavy water ( 2 H2O) media in which all hydrogen atoms are replaced with deuterium [1, 2]. 2 H2O has high environmental potential in biomedical studies due to the absence of radioactivity and poccebility of detecting the deuterium label in the molecule by high-resolution methods as NMR, IR, and mass spectrometry that facilitates its use as a tracer in biochemical and biomedical research [3]. The average ratio of 1 H/ 2 H in nature makes up approximately 1:5700 [4]. In natural waters, the deuterium is distributed irregularly: from 0,02-0,03 mol.% for river water and sea water, to 0,015 mol.% for water of Antarctic ice -the most purified from deuterium natural water containing in 1,5 times less deuterium than that of seawater. O = 1894 . 10 -6 (1894 ppm). In surface waters, the ratio 2 H/ 1 H = ∼(1,32-1,51) . 10 -4 , while in the coastal seawater -∼(1,55-1,56) . 10 -4 . The natural waters of CIS countries are characterized by negative deviations from SMOW standard to (1,0-1,5) . 10 -5 , in some places up to (6,0-6,7) . 10 -5 , but however there are also observed the positive deviations at 2,0 . 10 -5 .The chemical structure of 2 H2O molecule is analogous to that one for Н2O, with small differences in the length of the covalent H-O-bonds and the angles between them. The molecular mass of 2 H2O exceeds on 10% th...

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Biological Influence of Deuterium on Procariotic and Eukaryotic Cells

Cited by 16 publications

References 23 publications

Evolution, Metabolism and Biotechnological Usage of Methylotrophic Microorganisms

Evolution, Metabolism and Biotechnological Usage of Methylotrophic Microorganisms

Studying of Isotopic Effects of Deuterium in Biological Objects

Studying Physiological, Morphological, and Cytological Alterations in Prokaryotic and Eukaryotic Cells in Heavy Water

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