Biological characterization of clones derived from the edmonston strain of measles virus in comparison with schwarz and CAM-70 vaccine strains

Borges, Maria Beatriz; Mann, G. F.; Freire, Marcos da Silva

doi:10.1590/s0074-02761996000400020

Cited by 4 publications

(3 citation statements)

References 27 publications

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“…Transient expression with plasmids containing F and H protein regions from the AIK-C vaccine and Edmonston wild type at amino acid position 278 of the F gene demonstrated that leucine was responsible for reduced fusion capability and that phenylalanine was responsible for extensive cell fusion related to syncytium formation (28). Different plaque-forming variants have been isolated from wildtype virus or vaccine strains, including CAM-70 BM (29). Strains that do not form plaques but instead lead to the formation of clumps (foci) of infected cells have also been described (30).…”

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confidence: 99%

Comparative sequence analysis of the P-, M- and L-coding region of the measles virus CAM-70 live attenuated vaccine strain

Santos

Azevedo

Borges

et al. 2003

Braz J Med Biol Res

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Measles virus is a highly contagious agent which causes a major health problem in developing countries. The viral genomic RNA is singlestranded, nonsegmented and of negative polarity. Many live attenuated vaccines for measles virus have been developed using either the prototype Edmonston strain or other locally isolated measles strains. Despite the diverse geographic origins of the vaccine viruses and the different attenuation methods used, there was remarkable sequence similarity of H, F and N genes among all vaccine strains. CAM-70 is a Japanese measles attenuated vaccine strain widely used in Brazilian children and produced by Bio-Manguinhos since 1982. Previous studies have characterized this vaccine biologically and genomically. Nevertheless, only the F, H and N genes have been sequenced. In the present study we have sequenced the remaining P, M and L genes (approximately 1.6, 1.4 and 6.5 kb, respectively) to complete the genomic characterization of CAM-70 and to assess the extent of genetic relationship between CAM-70 and other current vaccines. These genes were amplified using long-range or standard RT-PCR techniques, and the cDNA was cloned and automatically sequenced using the dideoxy chain-termination method. The sequence analysis comparing previously sequenced genotype A strains with the CAM-70 Bio-Manguinhos strain showed a low divergence among them. However, the CAM-70 strains (CAM-70 Bio-Manguinhos and a recently sequenced CAM-70 submaster seed strain) were assigned to a specific group by phylogenetic analysis using the neighbor-joining method. Information about our product at the genomic level is important for monitoring vaccination campaigns and for future studies of measles virus attenuation.

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confidence: 99%

Comparative sequence analysis of the P-, M- and L-coding region of the measles virus CAM-70 live attenuated vaccine strain

Santos

Azevedo

Borges

et al. 2003

Braz J Med Biol Res

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“…For security reasons, the Schwarz vaccine strain of MV was used in the present study. Since 1954, when MV was first isolated in tissue culture (11), continuous monkey cell lines (e.g., Vero) have commonly been used for MV isolation (4). Vero cells were infected with the Schwarz strain of MV, and at 48 h postinfection multiple syncytia appeared (Fig.…”

Section: Results Andmentioning

confidence: 99%

Neutralization of Measles Virus Infectivity and Antibody-Dependent Cell-Mediated Cytotoxicity Activity against an Epstein-Barr Virus-Infected Cell Line by Intravenous Administration of Immunoglobulin G

Colomar¹,

Puga²,

López

et al. 2003

Clin Vaccine Immunol

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Characterization of the specific antimicrobial function of intravenous immunoglobulin G (i.v. IgG) preparations against particular microbial pathogens can assist in determining their therapeutic potential for specific infectious diseases. i.v. IgGs have been reported to contain antibodies directed against several viruses (24). However, the functionality of such antibodies against viral infections remains to be fully characterized.Measles virus (MV) causes an acute disease that still kills more than 1 million children in the less well developed world every year (29). The severity of measles in the young is mainly due to secondary infections (2, 9) as a result of immune suppression. The mechanism of immune suppression is due to apoptosis of infected hemopoietic cells (13) and interference with dendritic and T-cell functions (16).Measurable parameters of the immune response to MV infection include neutralization by antibody, antibody-dependent cell-mediated cytotoxicity (ADCC), antibody-dependent complement-mediated lysis, and cytotoxic T-lymphocyte activity (14, 15). The neutralizing antibody titer correlates well with protection from MV infection (6, 15).NK cell activity is crucial against infection by Epstein-Barr virus (EBV). Low NK cell cytotoxic activity is linked with increased human sensitivity to severe disseminating herpesvirus group infections, including those caused by herpes simplex virus (3, 7) and EBV (22,26). ADCC is thought to play a major role in controlling the spread of EBV in an infected individual.The viral membrane glycoprotein gp350/220, which is expressed at the surface of the virus-producing cell, was identified as a target for ADCC reactions (21). Sera from EBVpositive individuals provide antibodies for EBV-specific ADCC reactions (21). In individuals affected by X-linked lymphoproliferative disease, both the spontaneous NK cell cytotoxicity against EBV-infected cells and also EBV-infected cell lysis induced via CD16 are blocked (5,8,28,30,32,34). These individuals have a severe mononucleosis when they are infected with EBV. The gene modified in these patients codes for an NK cell coreceptor that is crucial for activation of cytotoxicity in NK and CD8 ϩ T cells. This demonstrates the importance that cytotoxic responses have for the control of EBV infections.i.v. IgG preparations contain significant levels of anti-MV and anti-EBV antibodies (24). However, the functionality of these antiviral antibodies has not been fully characterized. In the present work we have investigated the capacity of i.v. IgG to neutralize MV infectivity and to activate ADCC activity against an EBV-transformed cell line. The results indicate that i.v. IgG preparations contain a full capacity to neutralize MV and are also able to activate ADCC on lymphocyte preparations against the EBV-infected cell line Raji. MATERIALS AND METHODSReagents. Sorbitol (5%; pH 5 to 6) and human albumin (20%) were provided by Instituto Grifols S.A. (Parets, Spain). Dulbecco's phosphate-buffered saline (DPBS) and phosphate-buffered salin...

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“…Likewise, an F glycoprotein mutant could have been selected. Indeed, Borges et al have reported that the Schwarz MV strain differs from the Edmonston strain by its inability to lyse simian erythrocytes, a process known to involve the F protein after the H-CD46 interaction (1). Moreover, the Schwarz MV strain formed small plaques after infection of Vero cells, in contrast to the Hallé and Edmonston virus strains, which induced extensive cell-cell fusion (reference 1 and data not shown).…”

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confidence: 96%

Infection of Chicken Embryonic Fibroblasts by Measles Virus: Adaptation at the Virus Entry Level

Escoffier

Gerlier

1999

J Virol

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Measles virus (MV) has a tropism restricted to humans and primates and uses the human CD46 molecule as a cellular receptor. MV has been adapted to grow in chicken embryonic fibroblasts (CEF) and gave rise to an attenuated live vaccine. Hallé and Schwarz MV strains were compared in their ability to infect both simian Vero cells and CEF. Whereas both strains infected Vero cells, only the CEF-adapted Schwarz strain was able to efficiently infect CEF. Since the expression of the human MV receptor CD46 rendered the chicken embryonic cell line TCF more permissive to the infection by the Hallé MV strain, the MV entry into CEF appeared to be a limiting step in the absence of prior MV adaptation. CEF lacked reactivity with anti-CD46 antibodies but were found to express another protein allowing MV binding as an alternative receptor to CD46.

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Biological characterization of clones derived from the edmonston strain of measles virus in comparison with schwarz and CAM-70 vaccine strains

Cited by 4 publications

References 27 publications

Comparative sequence analysis of the P-, M- and L-coding region of the measles virus CAM-70 live attenuated vaccine strain

Comparative sequence analysis of the P-, M- and L-coding region of the measles virus CAM-70 live attenuated vaccine strain

Neutralization of Measles Virus Infectivity and Antibody-Dependent Cell-Mediated Cytotoxicity Activity against an Epstein-Barr Virus-Infected Cell Line by Intravenous Administration of Immunoglobulin G

Infection of Chicken Embryonic Fibroblasts by Measles Virus: Adaptation at the Virus Entry Level

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