Biological characteristics of stem cells derived from burned skin—a comparative study with umbilical cord stem cells

Abstract

Introduction Burned human skin, which is routinely excised and discarded, contains viable mesenchymal stromal/stem cells (burn-derived mesenchymal stromal/stem cells; BD-MSCs). These cells show promising potential to enable and aid wound regeneration. However, little is known about their cell characteristics and biological function. Objectives This study had two aims: first, to assess critical and cellular characteristics of BD-MSCs and, second, to compare those results with multipotent well-characterized MSCs from Wharton’s jelly of human umbilical cords (umbilical cord mesenchymal stromal/stem cells, UC-MSCs). Methods BD- and UC-MSCs were compared using immunophenotyping, multi-lineage differentiation, seahorse analysis for glycolytic and mitochondrial function, immune surface markers, and cell secretion profile assays. Results When compared to UC-MSCs, BD-MSCs demonstrated a lower mesenchymal differentiation capacity and altered inflammatory cytokine secretomes at baseline and after stimulation with lipopolysaccharides. No significant differences were found in population doubling time, colony formation, cell proliferation cell cycle, production of reactive oxygen species, glycolytic and mitochondrial function, and in the expression of major histocompatibility complex I and II and toll-like receptor (TLR). Importance, translation This study reveals valuable insights about MSCs obtained from burned skin and show comparable cellular characteristics with UC-MSCs, highlighting their potentials in cell therapy and skin regeneration.

Regeneration Potential of Mesenchymal Stem Cells in Cold Induced Burn Wounds

“…Conditioned medium of hUCMSCs was used to see whether it enhances the migration of broblasts towards the scratch and promotes wound healing. Various studies suggest that the conditioned medium contains growth factors or paracrine mediators which facilitate cells to migrate towards the wound site and help in wound contraction [9,11,28,29]. Our ndings are positively correlated with the reported studies as broblasts treated with conditioned medium have shown signi cantly accelerated cell migration towards the injury site and enhanced wound closure as compared to the untreated broblasts.…”

“…Stem cell therapy has emerged as a promising approach for chronic wounds including cold burns. hUCMSCs can be used in clinical applications for tissue regeneration and cutaneous wound healing [9]. They are known for having the characteristics of self-renewal, multipotent differentiation, and paracrine signaling [10,11].…”

Mesenchymal Stem Cells in Burn Wound Management

“…Dolp et al obtained stem cells from discarded burned skin without subcutaneous fat. In comparison with umbilical cord stem cells, there was no significant difference in the expression of MHC I and II, ROS release, or colony formation [ 74 ]. Van der Veen also obtained stem cells from the eschar [ 75 ].…”

Stem Cell Therapy for Burns: Story so Far

“…44 BD-MSCs extracted from full thickness burns function like other MSCs and are unaffected by thermal damage in terms of important cell functions involved in burn wound healing. 46…”