Biological aspects of the DM28C clone of Trypanosoma cruzi after metacylogenesis in chemically defined media

Contreras, Víctor; Araújo-Jorge, Tânia C.; Bonaldo, Myrna C.; Thomaz, Neide; Barbosa, Helene Santos; Meirelles, Maria de Nazareth Leal de; Goldenberg, Samuel

doi:10.1590/s0074-02761988000100016

Cited by 194 publications

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“…Parasites stocks -The Tulahuen (Pizzi 1957), SA strains, Dm 28c clone (Contreras et al 1988) of T. cruzi and Choachí-2V strain of T. rangeli were used. The SA strain was isolated in 1999, from Rhodnius pallescenses, in the San Antonio region, Magdalena, Colombia, where an outbreak of acute Chagas disease occurred that year, with several fatal human cases.…”

Section: Methodsmentioning

confidence: 99%

Action of Trypanosoma rangeli in infections with virulent Trypanosoma cruzi populations

Palau

Mejía

Vergara

et al. 2003

Mem. Inst. Oswaldo Cruz

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Section: Methodsmentioning

confidence: 99%

Action of Trypanosoma rangeli in infections with virulent Trypanosoma cruzi populations

Palau

Mejía

Vergara

et al. 2003

Mem. Inst. Oswaldo Cruz

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“…T. cruzi clone Dm28c, isolated from opossum (Contreras et al, 1988) was used in this study. Parasites were maintained by weekly passages in liver infusion tryptose medium (LIT) (Camargo, 1964); epimastigotes were transformed into metacyclic trypomastigotes in artificial triatomine urine medium (TAU) (I90 mM-NaC1, 17 mM-KCl, 2 mM-MgCl,, 2 mM-CaC12, 8 mM-potassium phosphate buffer, pH 6.0) supplemented with 10 mM-L-proline (TAUP) as previously described (Contreras et al, 1985).…”

Section: Methodsmentioning

confidence: 99%

“…1985 ; Bonaldo et al, 1988). In addition to morphological changes, the metacyclogenesis process involves differential gene expression (Contreras et al, 1985;Bonaldo et al, 1988) and changes in fatty acid composition (Esteves et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

“…In addition to morphological changes, the metacyclogenesis process involves differential gene expression (Contreras et al, 1985;Bonaldo et al, 1988) and changes in fatty acid composition (Esteves et al, 1989). The process also involves changes in surface carbohydrates, since epimastigotes and trypomastigotes display different lectin agglutination profiles (Pereira et al, 1980).…”

Section: Introductionmentioning

confidence: 99%

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Changes in cell-surface carbohydrates of Trypanosoma cruzi during metacyclogenesis under chemically defined conditions

Andrade¹,

Esteves²,

Angluster³

et al. 1991

Journal of General Microbiology

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Highly purified lectins with specificities for receptor molecules containing sialic acid, N-acetylglucosamine (DGlcNAc), N-acetylgalactosamine (D-GalNAc), galactose (D-Gal), mannose-like residues (D-Man) or L-fucose (LFuc), were used to determine changes in cell-surface carbohydrates of the protozoal parasite Trypanosomu cruzi during metacyclogenesis under chemically defined conditions. Of the D-GalNAc-binding lectins, BS-I selectively agglutinated metacyclic trypomastigotes, MPL was selective for replicating epimastigotes, whereas SBA strongly agglutinated all developmental stages of T. cruzi. WGA (sialic acid and/or D-GIcNAc specific) was also reactive with differentiating epimastigotes and metacyclic trypomastigotes but displayed a higher reactivity with replicating epimastigote forms. A progressive decrease in agglutinating activity was observed for jacaline (specific for D-Gal) during the metacyclogenesis process; conversely, a progressive increase in affinity was observed for RCA-I (D-Gabspecific), although the reactivity of other D-Gal-specific lectins (PNA and AxP) was strong at all developmental stages. All developmental stages of T. cruzi were agglutinated by Con A and Lens cufinaris lectins (specific for D-Man-like residues); however, they were unreactive with the L-fucose-binding lectins from Lotus tetrugonolobos and Ufex europueus. These agglutination assays were further confirmed by binding studies using I z51-labelled lectins. Neuraminidase activity was detected in supernatants of cell-free differentiation medium using the PNA hemagglutination test with human A erythrocytes. The most pronounced differences in lectin agglutination activity were observed between replicating and differentiating epimastigotes, suggesting that changes in the composition of accessible cell-surface carbohydrates precede the morphological transformation of epimastigotes into metacyclic trypomastigotes.

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“…Parasite culture and stress conditions -Epimastigotes of T. cruzi clone Dm28c (Contreras et al 1988) were maintained in the exponential growth phase by subculturing approximately 2 x 10 7 cells/mL every three days at 28ºC in liver infusion tryptose (LIT) medium supplemented with 10% foetal bovine serum (Camargo 1964). Epimastigotes in late growth phase were cultured for five days under the same conditions.…”

Section: Methodsmentioning

confidence: 99%

Characterization of TcSTI-1, a homologue of stress-induced protein-1, in Trypanosoma cruzi

Schmidt

Soares

Goldenberg

et al. 2011

Mem. Inst. Oswaldo Cruz

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Key words: STI-1 -Hop -HSP70 -stress response -Trypanosoma cruziThe stress-induced protein-1 (STI-1), also known as Hop (HSP70/HSP90-organising protein), is a co-chaperone that interacts with the heat-shock proteins HSP70 and HSP90 (Smith et al. 1993). This multi-chaperone complex is required for steroid receptor (SR) assembly (Chen et al. 1996, Dittmar et al. 1996, Chang et al. 1997 in which STI-1 acts as a mediator, potentially increasing the rate of SR maturation (Morishima et al. 2000). Furthermore, this chaperone complex is able to reconstitute hepadnavirus reverse transcriptase activity in vitro (Hu et al. 2002), suggesting that a purified assembly system could be used to mediate various biological processes requiring HSP90 function (Pratt & Toft 2003).STI-1 acts as an adaptor protein, mediating the interaction between HSP70 and HSP90 through its tetratricopeptide repeat (TPR) domains. The N-terminal TPR domain (TPR1) specifically recognizes the C-terminus of HSP70, whereas the central TPR domain (TPR2A) binds the C-terminus of HSP90 (Chen & Smith 1998, Scheufler et al. 2000. The C-terminal domain of STI-1 seems to be involved in the interactions with both HSPs (Flom et al. 2007, Onuoha et al. 2008. STI-1 may also modulate HSP activities (Johnson et al. 1998, Richter et al. 2003, Odunuga et al. 2004). The STI-1 gene was first isolated and characterized in Saccharomyces cerevisiae during a genetic screen for proteins involved in the heat-shock response. The gene was named "stress-inducible" because, like other wellcharacterized HSP genes, it was induced by heat shock and by the arginine analogue canavanine. However, the nucleotide sequence of STI-1 displayed no similarity to the sequences of other HSP-encoding genes (Nicolet & Craig 1989). Like their counterpart in yeast, the mouse and Caenorhabditis elegans STI-1 homologues are induced by heat shock (Lässle et al. 1997, Song et al. 2009). Interestingly, a soybean homologue has been shown to be induced by either heat or cold stress (Zhang et al. 2003). In addition, the upregulation of STI-1 observed in human SV-40-transformed fibroblasts or mouse macrophages exposed to bacterial lipopolysaccharide demonstrates that stress conditions unrelated to temperature may also lead to the induction of STI-1 expression (Honoré et al. 1992, Heine et al. 1999. The murine STI-1 was identified as a cytoplasmic protein (Lässle et al. 1997). However, a small fraction of STI-1 was found bound to a cellular prion protein at the cell membrane, where it promotes neuroprotection against cell death (Zanata et al. 2002). Moreover, STI-1 accumulates in the nucleus of murine fibroblasts after treatment with leptomycin B, a nuclear export inhibitor . A human homologue has also been found in the Golgi apparatus and in small vesicles within fibroblasts (Honoré et al. 1992).A protozoan orthologue of STI-1 has been characterized in Leishmania major and shown to be upregulated when the parasites are exposed to heat stress conditions (Webb et al. 1997). This parasite, like other trypanoso-S...

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Biological aspects of the DM28C clone of Trypanosoma cruzi after metacylogenesis in chemically defined media

Cited by 194 publications

References 0 publications

Action of Trypanosoma rangeli in infections with virulent Trypanosoma cruzi populations

Action of Trypanosoma rangeli in infections with virulent Trypanosoma cruzi populations

Changes in cell-surface carbohydrates of Trypanosoma cruzi during metacyclogenesis under chemically defined conditions

Characterization of TcSTI-1, a homologue of stress-induced protein-1, in Trypanosoma cruzi

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