Biological and isoenzymatic characterization of Trypanosoma cruzi strains isolated from sylvatic reservoirs and vectors from the state of Santa Catarina, Southern Brazil

Steindel, Mário; Toma, Helena Keiko; Ishida, María Akíko; Murta, Silvane Maria Fonseca; Pinto, C.; Grisard, Edmundo C.; Schlemper, Bruno Rodolfo; Ribeiro‐Rodrigues, Rodrigo; Romanha, Álvaro J.

doi:10.1016/0001-706x(95)00124-w

Cited by 18 publications

(12 citation statements)

References 23 publications

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“…These results agree with those of other authors who have reported that more than 80% of naturally occurring strains have low or medium virulence for mice, as infectivity is an important parameter of virulence (Andrade 1974;Schlemper et al 1986;Carneiro et al 1991;Gomes et al 1995Gomes et al , 2003Steindel et al 1995;Fernandes et al 1997;Araújo et al 1999;Devera et al 2002;Toledo et al 2002).…”

Section: Discussionsupporting

confidence: 92%

Infectivity for mice of Trypanosoma cruzi I and II strains isolated from different hosts

et al. 2006

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In this paper, the infectivity for mice of Trypanosoma cruzi I and II strains isolated from sylvatic animals, triatomines, and humans is determined using fresh blood examination, hemoculture, culture of macerated organs, and polymerase chain reaction (PCR). Six strains were considered to have low infectivity (9.1-18.2%), five medium (27.3-45.4%), and one high (100.0%). Infectivity of T. cruzi strains isolated from sylvatic animals was significantly higher than that of strains isolated from humans and triatomines (p=0.0141). No significant difference was observed between the infectivity of T. cruzi I and II strains. The parasite was detected by fresh blood examination in one strain, by hemoculture and culture of macerated organs in four strains, and by PCR in all strains. We conclude that the infectivity is related to the host from which the strains were isolated, but the infectivity is not related to the genetic group of the parasite. We also conclude that the majority of the strains studied have low and medium infectivity for mice, and that PCR is an important tool to detect T. cruzi in strains with this biological characteristic.

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Section: Discussionsupporting

confidence: 92%

Infectivity for mice of Trypanosoma cruzi I and II strains isolated from different hosts

et al. 2006

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“…In southern Brazil especially in Santa Catarina, where the parasite was isolated from silvatic vectors or vertebrate hosts with no human cases being registered, Type I (Z2b) and III (Z1) strains were identified, confirming data of Steindel et al 32 . The small number of strains from other countries of South and Central America was not sufficient to evaluate the distribution of the strain Types and zymodemes, but confirmed findings from other authors, such as Apt et al 10 in Chile and Tibayrenc et al 34 in Bolivia, who registered the presence of Z2 and genetic variants in the domestic cycle and Z1 in the silvatic reservoirs; in the present study the three biodemes (I, II, III) have been detected in the several countries.…”

Section: Figure 5 -Myoenteric Plexus In Mice Infected With Type I Strsupporting

confidence: 83%

Biodemes and zymodemes of Trypanosoma cruzi strains: correlations with clinical data and experimental pathology

Andrade

Magalhães²

1997

Rev. Soc. Bras. Med. Trop.

137

112

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With the objective of establishing biological and biochemical characteristics of a significant number of Trypanosoma cruzi strains from different geographical areas, 138 strains isolated from naturally infected humans, triatomine or vertebrate hosts were studied; 120 were isolated from different areas of Brazil and 18 from other South and Central American countries. Inocula from triatomine or culture forms were injected into suckling Swiss mice, followed by passages into mice 10 to 12 g. Biological characters and histopathological study permitted the inclusion of the strains into three Types or biodemes: I, II, III. Isoenzymic analysis confirmed a correspondence between the biodemes and zymodemes : Type I and Z2b, Type II and Z2, Type III and Z1. Results showed the ubiquitary distribution of the several types of strains. The predominance of the same Type and zymodeme in one geographical area was confirmed : Type II strains among the human cases from eastern Bahia and east of Goiás; Type III strains from humans of north Brazil and Central America and from silvatic vectors or vertebrates from other geographical areas. The biological types of strains correlate with different histopathological lesions considering cardiac involvement and neuronal lesions. These findings suggest that the biological behavior together with isoenzymes patterns and pathological pictures in the vertebrate host can be an important tool for establishing correlations between strains behavior and clinico-pathological manifestations of Chagas' disease in different geographical areas.

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“…This intriguing intraspecific variation has been extensively investigated by biological characterization. One widely used method is isoenzyme analysis, which has revealed different populations between the zymodeme and the transmission cycle (Miles et al 1977(Miles et al , 1978Ebert 1982;Schottelius and Muller 1984;Ebert 1985;Tibayrenc and Ayala 1988;Steindel et al 1995). Also, lectins with different specificities for surface-membrane carbohydrate residues were used for taxonomic purposes (Miercio et al 1980;Marinkelle et al 1986).…”

Section: Introductionmentioning

confidence: 99%

Biochemical characterization of new strains of Trypanosoma cruzi and T. rangeli isolates from Peru and Mexico

et al. 2004

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Seven trypanosome stocks isolated have been characterized by lectin agglutination, isoenzyme analysis, and the end products excreted. The stocks were isolated from different geographic areas-one from Mexico (TM5), and six from Peru, four of these isolated from different species of triatoma (TP504, TP702, TP704 and TP706), the other two isolated from the salivary glands of Rhodnius ecuadorensis (TRa605 and TRa606). Additionally, one strain of Trypanosoma cruzi isolated from a human case (strain TC-Maracay) and one strain of T. rangeli (TRa, Cajamarca-Peru strain), characterized and maintained in our laboratory, were used as reference strains. According to statistical study, the stocks were grouped into three clusters: (1) cluster I included the reference strain of T. cruzi (TC-Maracay); (2) cluster II was subdivided into two groups-subcluster IIA for the Mexican isolate (TM5) and subcluster IIB for the Peruvian ones, isolated from the salivary glands of Rhodnius ecuadorensis (TRa 605 and TRa 606) and the reference strain T. rangeli (TRa); these two new isolates were classified as T. rangeli; and (3) cluster III for the rest of the Peruvian isolates, which should be considered at least as a different strain from the T. cruzi strain Maracay. We show that the identification of T. cruzi and T. rangeli in mixed infections is readily achieved by biochemical methods. These findings identified three clusters of Mexican and Peruvian stocks that correlate with geographic origin, although assignment to a T. cruzi linage was not possible.

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Biological and isoenzymatic characterization of Trypanosoma cruzi strains isolated from sylvatic reservoirs and vectors from the state of Santa Catarina, Southern Brazil

Cited by 18 publications

References 23 publications

Infectivity for mice of Trypanosoma cruzi I and II strains isolated from different hosts

Infectivity for mice of Trypanosoma cruzi I and II strains isolated from different hosts

Biodemes and zymodemes of Trypanosoma cruzi strains: correlations with clinical data and experimental pathology

Biochemical characterization of new strains of Trypanosoma cruzi and T. rangeli isolates from Peru and Mexico

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