Biologic properties and detection of immune complexes in animal and human pathology

Nydegger, Urs E.

doi:10.1007/bfb0036116

Cited by 9 publications

(5 citation statements)

References 190 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…La creatinina serica se mantuvo normal en todos los grupos, pero la excretion de eritrocitos aumento considerablemente en ratones, inyectados con 5 Estudio Experimental B: La creatinina serica se mantuvo normal (0.5 a 0.6 mg/ml) en todos los grupos. El examen de orina de ratones jovenes (2 meses) demostro proteinuria minima (trazas a 1+) mientras que en ratones de 12 a 24 meses de edad se detecto consistentemente en concentraciones de 2+ o mas.…”

Section: Resultsunclassified

See 1 more Smart Citation

Glomerulitis Inducida en Ratones Mediante Inmunocomplejos (1C): Efecto del Liquido Amniótico (LA) Sobre la Depuración de 1C y la Histopatología Renal

Cerdá¹,

E²,

Kaplan³

et al. 1985

Rev. chil. pediatr.

View full text Add to dashboard Cite

A normal clearance of immune complexes (1C), measured as phagocytic index, was observed in C57L/J mice 10 min after one dose of ' 2 s I-1C. Similarly, tissue uptake of radioactivity (percent of dose) was within normal limits when evaluated 15 and 120 min after injection. Daily dose of 1C (for 5 days) or pretreatment of mice with amniotic fluid containing alpha-fetoprotein (MAF/AFP) reduced significantly (p <0.01) both phagocytic index and percent of dose at 15 min when only one dose of 1C wasgrven.-Conversely, at 120 min the percent of dose in liver and kidney was incresed (p <0.01). In untreated mice the percent of dose was low at 120 min. Pietreatment with AFP-poor MAF had no effect upon clearance or percent of dose. Multiple injections 5 of 1C induced mesangial hyperplasia and infiltration-with polymorphonucleai and mononuclear leukocytes. Distinct granular-nodular fluorescent deposits developed in mesangium and peripheral capillary loops which correlated well with the electron dense material localized in mesangium. Pretreatment with MAF/AFP decreased significantly or prevented these lesions. Pretreatment with AFP -poor -MAF did not modify the inmunohistopathology. These previously unreported effects of MAF/AFP may represent inhibition of the reticuloendothelium. It is postulated that the increased radioactivity detected at 120 min may reflect reduced degradation of 1C as opposed to increased uptake. En la mayoria de las glomerulonefritis (GN), el mecanismo desencadenante envuelve la production de Inmuno-complejos (1C) 1 ' 7 . Ya sea que los 1C se formen en la circulation o "in situ" se desarrolla evidencia cli'nica e histopatologica de GN si la dosis del anti'geno, la formation de anticuerpos por el huesped y los procesos que facilitan la localization de antigenos o de 1C son adecuados 1 -7 -9 -11 • is•i4_La administration de 1C preformados es capaz de desencadenar GN, aunque sus resultados no son tan reproducibles como los obtenidos con inmunizacion activa en el animal experimental 1 7 ' 12 . La activaci6n del sistema reticuloendotelial (SRE), que fagocita y depura los 1C, puede, en forma efectiva, evitar que estos se depositen en los tejidos 15 " 18 . Sin embargo no se ha establecido claramente si la inhibition del SRE facilita o disminuye el deposito de 1C en el riflon 1 ~ 19 " 20 . Aun mas, la interpretation de la histopatologia renal en roedores se complica debido a que estos animales desarrollan espontaneamente lesiones glomerulares con inmunofluorescencia que sugiere un fenomeno mediado por la localization capilardeIC 1 -4-21-28 Los objetivos del presente estudio evaluan, primero: la funcion del SRE, la inmunohistopatologia y ultraestructura renal de ratones C57L/J inyectados con 1C preformados. Segundo, el efecto del LA (Liquido Amniotico) que contiene alfafetoproteina (AFP, LA/AFP), una substancia posiblemente inmunosupresora 29 sobre la funcion depuradora de 1C del SRE. Por ultimo, se realizaron estudios morfometricos y longitudinales, a traves de varias edades, en ratones C57L/J con el objeto de c...

show abstract

Section: Resultsunclassified

“…La radioactividad Vol. 56 NO 5 de los tejidos se calculo pesando 2 muestras del organo en particular obteniendose la radioactividad por gramo tejido. Este resultado fue multiplicado por el peso del organo para indicar la radioactividad total del organo.…”

Section: Materials Y Metodounclassified

Glomerulitis Inducida en Ratones Mediante Inmunocomplejos (1C): Efecto del Liquido Amniótico (LA) Sobre la Depuración de 1C y la Histopatología Renal

Cerdá¹,

E²,

Kaplan³

et al. 1985

Rev. chil. pediatr.

View full text Add to dashboard Cite

show abstract

“…basement membrane. Such deposits have been seen in glomerular basement membranes as well as in skin (Nydegger 1979}.…”

Section: Blood-testis Barriermentioning

confidence: 93%

“…An excess of antigen causes the formation of immune complexes that are too small to lodge an~where and consequently stay in the circulation; an excess of antibody causes the formation of large inunune complexes that are disposed of by the mononuclear phagocytic system. 30 A proportion of antibody to antigen greater than 1 would give rise to precipitates since an excess of antibody molecules allows the formation of steric aggregates of considerable volume and these spontaneously settle out from solution (Nydegger 1979 (Wells 1978).…”

Section: Immune Complex Formationmentioning

confidence: 99%

The ductuli efferentes in Macaca mulatta : electron microscopic evaluation of changes after vasectomy

Marsh¹

2000

View full text Add to dashboard Cite

“…Circulating immune complexes have been reported in a wide variety of diseases, including glomerulonephritis, rheumatoid arthritis, systemic lupus and many bacterial, viral and parasitic infections (Nydegger, 1979;Wands et al. 1975).…”

mentioning

confidence: 99%

Characterization of a lung‐cancer‐associated auto‐antigen

Zeng

Alpert

1992

Intl Journal of Cancer

View full text Add to dashboard Cite

An antigen, protein X (Px), was purified from immune complexes isolated from malignant pleural effusions from patients with adenocarcinoma of the lung by EDTA treatment, PEG 8000 precipitation, protein A affinity chromatography, and Sephadex G-200 separation in the presence of 3 M NaCl. The purified antigen had a M(r) 17,000 by SDS-PAGE, and consisted of isoelectric species of pI 6.3 and 6.6. Purified Px recombined with Ig isolated from pleural fluids from patients with lung adenocarcinoma, but not with Ig from patients with breast carcinoma. Using an autologous human and heterologous chicken antibody, Px was found, by immunohistology, in the cytoplasm of some of the well-differentiated lung adenocarcinoma cells, but was not seen in normal lung or a variety of other malignant tissues. A liquid-phase competitive-inhibition RIA was developed. Over 30 ng/ml of Px were found in 9 of 15 pleural fluids from patients with lung carcinoma, none of 20 from patients with breast, ovary, stomach or colon cancer, and in 3 of 15 patients with unknown primary tumor. Our data suggest that Px may be a lung-cancer-associated autoantigen which can elicit a host humoral response in vivo.

show abstract

Biologic properties and detection of immune complexes in animal and human pathology

Cited by 9 publications

References 190 publications

Glomerulitis Inducida en Ratones Mediante Inmunocomplejos (1C): Efecto del Liquido Amniótico (LA) Sobre la Depuración de 1C y la Histopatología Renal

Glomerulitis Inducida en Ratones Mediante Inmunocomplejos (1C): Efecto del Liquido Amniótico (LA) Sobre la Depuración de 1C y la Histopatología Renal

The ductuli efferentes in Macaca mulatta : electron microscopic evaluation of changes after vasectomy

Characterization of a lung‐cancer‐associated auto‐antigen

Contact Info

Product

Resources

About