Bioinformatic Analysis of Human Cumulus Cells to Unravel Cellular’s Processes that Could Be Used to Establish Oocyte Quality Biomarkers with Clinical Application

Mengden, Lúcia von; Bastiani, Marco Antônio De; Grün, Lucas Kich; Barbé-Tuana, Florencia Marı́a; Adriaenssens, Tom; Smitz, Johan; Arruda, Leticia Schmidt; Link, Carlos Alberto; Klamt, Fábio

doi:10.1007/s43032-022-01046-2

Cited by 4 publications

(8 citation statements)

References 75 publications

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“…In the daily practice of ICSI, oocyte quality is evaluated by morphological parameters, including cytoplasmic granularity, zona pellucida, perivitelline space, etc. ; however, numerous studies have shown that human oocyte morphology is not significantly associated with competent, fertilization, subsequent embryo development or pregnancy outcomes. − Therefore, new techniques assessing oocyte quality are emerging, such as mitochondrial DNA copy number and transcript analysis of cumulus cells . It should be noted that these methods are invasive, which hinders their clinical application in the field of ART.…”

Section: Introductionmentioning

confidence: 99%

“…5−7 Therefore, new techniques assessing oocyte quality are emerging, such as mitochondrial DNA copy number 8 and transcript analysis of cumulus cells. 9 It should be noted that these methods are invasive, which hinders their clinical application in the field of ART. Follicular fluid metabolism is unharmful to oocytes, but its accuracy is probably limited.…”

Section: ■ Introductionmentioning

confidence: 99%

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Dual-Activated H₂O₂-Responsive AIE Probes for Oocyte Quality Assessment

Dong,

Ouyang,

Lou

et al. 2024

Anal. Chem.

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Nonobstructive azoospermia (NOA) is an important cause of infertility, and intracytoplasmic sperm injection (ICSI) is the mainstay of treatment for these patients. In cases where a sufficient number of sperm (usually 1−2) is not available, the selection of oocytes for ICSI is a difficult problem that must be solved. Here, we constructed a dual-activated oxidative stress-responsive AIE probe, b-PyTPA. The strong donor−acceptor configuration of b-PyTPA leads to twisted intramolecular charge transfer (TICT) effect that quenches the fluorescence of the probe, however, H 2 O 2 would specifically remove the boronatebenzyl unit and release a much weaker acceptor, which inhibits TICT and restores the fluorescence. In addition, the presence of a pyridine salt makes b-PyTPA more hydrophilic, whereas removal of the pyridine salt increases the hydrophobicity of PyTPA, which triggers aggregation and further enhances fluorescence. Thus, the higher the intracellular level of oxidative stress, the stronger the fluorescence. In vitro, this dual-activated fluorescent probe is capable of accurately detecting senescent cells (high oxidative stress). More importantly, b-PyTPA was able to characterize senescent oocytes, as assessed by the level of oxidative stress. It is also possible to identify high quality oocytes from those obtained for subsequent ICSI. In conclusion, this dual-activated oxidative stress-assessment probe enables the quality assessment of oocytes and has potential application in ICSI.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Dual-Activated H₂O₂-Responsive AIE Probes for Oocyte Quality Assessment

Dong,

Ouyang,

Lou

et al. 2024

Anal. Chem.

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show abstract

Section: Introductionmentioning

confidence: 99%

“…Although very promising, assisted reproductive technologies (ART) are still inefficient in terms of overall application [ 5 ], and their outcomes are hardly predictable [ 5 , 6 ]. Although different variables need to be considered to define the success of ART, oocyte quality and selection are considered crucial, limiting embryo overproduction and shortening the time for take-home baby rates [ 5 , 7 ]. Furthermore, oocyte evaluation based on morphological criteria is still a challenge [ 7 , 8 , 9 , 10 ], although some non-invasive objective biomarkers of oocyte quality have been described [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

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Mitochondrial Dysfunction in Advanced Maternal Aged Cumulus Cells: A Possible Link to ATP Synthase Impairment?

Almeida-Reis,

Carvalho,

Dias

et al. 2024

Biomolecules

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Age-related changes in the mitochondrial status of human cumulus cells (hCCs) impact oocyte quality; however, the relationship between hCC mitochondrial (dys)function and reproductive aging remains poorly understood. This study aimed to establish the interplay between hCC mitochondrial dysfunction and women’s reproductive potential. In this investigation, 266 women were enrolled and categorized into two groups based on their age: a young group (<35 years old) and an advanced maternal age (AMA) group (≥35 years old). Comprehensive analysis of reproductive outcomes was conducted in our population. Various mitochondrial-related parameters were analyzed across distinct subsets. Specifically, mitochondrial membrane potential (∆Ψm) and mitochondrial mass were examined in 53 samples, mtDNA content in 25 samples, protein levels in 23 samples, bioenergetic profiles using an XF24 Extracellular Flux Analyzer in 6 samples, and levels of reactive oxygen species (ROS) and adenosine triphosphate (ATP) in 39 and 43 samples, respectively. In our study, the reproductive potential of AMA women sharply decreased, as expected. Additionally, an impairment in the mitochondrial function of hCCs in older women was observed; however, no differences were found in terms of mitochondrial content. Regarding oxidative phosphorylation, metabolic profiling of hCCs from AMA women indicated a decrease in respiratory capacity, which was correlated with an age-dependent decrease in the ATP synthase (ATP5A1) protein level. However, intracellular ROS and ATP levels did not differ between groups. In conclusion, our study indicates that age-related dysfunction in hCCs is associated with impaired mitochondrial function, and, although further studies are required, ATP synthase could be relevant in this impairment.

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Transcriptomic Analysis and Finding of Potential Key mRNA Expression Profile in Human Cumulus Cells During in Vitro Culture and Different Passages Based on Integrated Bioinformatics Analysis

Wang,

2024

Reprod. Sci.

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Bioinformatic Analysis of Human Cumulus Cells to Unravel Cellular’s Processes that Could Be Used to Establish Oocyte Quality Biomarkers with Clinical Application

Cited by 4 publications

References 75 publications

Dual-Activated H₂O₂-Responsive AIE Probes for Oocyte Quality Assessment

Dual-Activated H₂O₂-Responsive AIE Probes for Oocyte Quality Assessment

Mitochondrial Dysfunction in Advanced Maternal Aged Cumulus Cells: A Possible Link to ATP Synthase Impairment?

Transcriptomic Analysis and Finding of Potential Key mRNA Expression Profile in Human Cumulus Cells During in Vitro Culture and Different Passages Based on Integrated Bioinformatics Analysis

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Bioinformatic Analysis of Human Cumulus Cells to Unravel Cellular’s Processes that Could Be Used to Establish Oocyte Quality Biomarkers with Clinical Application

Cited by 4 publications

References 75 publications

Dual-Activated H2O2-Responsive AIE Probes for Oocyte Quality Assessment

Dual-Activated H2O2-Responsive AIE Probes for Oocyte Quality Assessment

Mitochondrial Dysfunction in Advanced Maternal Aged Cumulus Cells: A Possible Link to ATP Synthase Impairment?

Transcriptomic Analysis and Finding of Potential Key mRNA Expression Profile in Human Cumulus Cells During in Vitro Culture and Different Passages Based on Integrated Bioinformatics Analysis

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Dual-Activated H₂O₂-Responsive AIE Probes for Oocyte Quality Assessment

Dual-Activated H₂O₂-Responsive AIE Probes for Oocyte Quality Assessment