Biogenic carbon dots: a novel mechanistic approach to combat multidrug-resistant critical pathogens on the global priority list

Parambil, Ajith Manayil; Prasad, Abhinav; Tomar, Anuj Kumar; Ghosh, Ilora; Rajamani, Paulraj

doi:10.1039/d3tb02374e

J. Mater. Chem. B

2024

DOI: 10.1039/d3tb02374e

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Biogenic carbon dots: a novel mechanistic approach to combat multidrug-resistant critical pathogens on the global priority list

Ajith Manayil Parambil,

Abhinav Prasad,

Anuj Kumar Tomar

et al.

Abstract: This study delves into investigating alternative methodologies for anti-microbial therapy by focusing on the mechanistic assessment of carbon dots (CDs) synthesized from F. benghalensis L. extracts.

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Cited by 4 publications

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Naringenin Against Cadmium Toxicity in Fibroblast Cells: An Integrated Network Pharmacology and In Vitro Metabolomics Approach

Priya,

Roy,

Prasad

et al. 2024

Environmental Toxicology

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Cadmium, a heavy metal, disrupts cellular homeostasis and is highly toxic, with no effective treatments currently available against its toxicity. According to studies, phytochemicals provide a promising strategy for mitigating cadmium toxicity. Naringenin (NG), a potent antioxidant found primarily in citrus fruits, showed protective properties against cadmium toxicity in rats. Nonetheless, the precise mechanism of cadmium cytotoxicity in fibroblasts remains unknown. This study evaluated NG against cadmium (CdCl2) toxicity utilizing network pharmacology and in silico molecular docking, and was further validated experimentally in rat fibroblast F111 cells. Using network pharmacology, 25 possible targets, including the top 10 targets of NG against cadmium, were identified. Molecular docking of interleukin 6 (IL6), the top potential target with NG, showed robust binding with an inhibition constant (Ki) of 58.76 μM, supporting its potential therapeutic potential. Pathway enrichment analysis suggested that “response to reactive oxygen species” and “negative regulation of small molecules metabolic process” were the topmost pathways targeted by NG against cadmium. In vitro analysis showed that NG (10 μM) attenuated CdCl2‐induced oxidative stress by reducing altered intracellular ROS, mitochondrial mass, and membrane potential. Also, NG reversed CdCl2‐mediated nuclear damage, G2/M phase arrest, and apoptosis. GC/MS‐based metabolomics of F111 cells revealed CdCl2 reduced cholesterol levels, which led to alterations in primary bile acid, steroid and steroid hormone biosynthesis pathways, whereas, NG restored these alterations. In summary, combined in silico and in vitro analysis suggested that NG protected cells from CdCl2 toxicity by mitigating oxidative stress and metabolic pathway alterations, providing a comprehensive understanding of its protective mechanisms against cadmium‐induced toxicity.

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Naringenin Against Cadmium Toxicity in Fibroblast Cells: An Integrated Network Pharmacology and In Vitro Metabolomics Approach

Priya,

Roy,

Prasad

et al. 2024

Environmental Toxicology

View full text Add to dashboard Cite

show abstract

Quantum dots: a next generation approach for pathogenic microbial biofilm inhibition; mechanistic insights, existing challenges, and future potential

Koul,

Jawanda,

Soni

et al. 2024

Arch Microbiol

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Transcriptomic and Molecular Docking Analysis Reveal Virulence Gene Regulation-Mediated Antibacterial Effects of Benzyl Isothiocyanate Against Staphylococcus aureus

Liu,

Zhu,

Hao

et al. 2024

Appl Biochem Biotechnol

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Biogenic carbon dots: a novel mechanistic approach to combat multidrug-resistant critical pathogens on the global priority list

Abstract: This study delves into investigating alternative methodologies for anti-microbial therapy by focusing on the mechanistic assessment of carbon dots (CDs) synthesized from F. benghalensis L. extracts.

Cited by 4 publications

References 59 publications

Naringenin Against Cadmium Toxicity in Fibroblast Cells: An Integrated Network Pharmacology and In Vitro Metabolomics Approach

Naringenin Against Cadmium Toxicity in Fibroblast Cells: An Integrated Network Pharmacology and In Vitro Metabolomics Approach

Quantum dots: a next generation approach for pathogenic microbial biofilm inhibition; mechanistic insights, existing challenges, and future potential

Transcriptomic and Molecular Docking Analysis Reveal Virulence Gene Regulation-Mediated Antibacterial Effects of Benzyl Isothiocyanate Against Staphylococcus aureus

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