Biofilm formation in geometries with different surface curvature and oxygen availability

Chang, Ya Wen; Fragkopoulos, Alexandros; Marquez, Samantha M.; Kim, Harold D.; Angelini, Thomas E.; Fernández‐Nieves, Alberto

doi:10.1088/1367-2630/17/3/033017

Cited by 56 publications

(29 citation statements)

References 28 publications

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“…Quantitative biofilm structural parameters were calculated, including (1) mean thickness, which identifies the mean distance from the substratum in the direction normal to the substratum where there is biofilm; (2) roughness, a quantity calculated from the thickness distribution and which describes the heterogeneity of the biofilm; (3) substratum coverage, the percentage of substratum area occupied by the biofilm; (4) surface-to-volume ratio, which reflects the fraction of the biofilm area that is exposed to the nutrients; and (5) bio-volume, of the cell polysaccharide matrix, which provides an estimation of the biomass in the biofilm (Chang et al 2015). Biofilm morphological parameters were obtained via MetaMorph 7.5 (Molecular Devices) and COMSTAT software from at least five random images for each sample according to Heydorn et al (2000).…”

Section: Confocal Laser Scanning Microscopy (Clsm) Analysismentioning

confidence: 99%

Zosteric acid and salicylic acid bound to a low density polyethylene surface successfully control bacterial biofilm formation

et al. 2018

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The active moieties of the anti-biofilm natural compounds zosteric (ZA) and salicylic (SA) acids have been covalently immobilized on a low density polyethylene (LDPE) surface. The grafting procedure provided new non-toxic eco-friendly materials (LDPE-CA and LDPE-SA) with anti-biofilm properties superior to the conventional biocide-based approaches and with features suitable for applications in challenging fields where the use of antimicrobial agents is limited. Microbiological investigation proved that LDPE-CA and LDPE-SA: (1) reduced Escherichia coli biofilm biomass by up to 61% with a mechanism that did not affect bacterial viability; (2) significantly affected biofilm morphology, decreasing biofilm thickness, roughness, substratum coverage, cell and matrix polysaccharide bio-volumes by >80% and increasing the surface to bio-volume ratio; (3) made the biofilm more susceptible to ampicillin and ethanol. Since no molecules were leached from the surface, they remained constantly effective and below the lethal level; therefore, the risk of inducing resistance was minimized.

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Section: Confocal Laser Scanning Microscopy (Clsm) Analysismentioning

confidence: 99%

Zosteric acid and salicylic acid bound to a low density polyethylene surface successfully control bacterial biofilm formation

et al. 2018

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“…We fabricate toroidal gels by first forming toroidal droplets of a precursor NIPAM solution, which is then UV-polymerized [12][13][14]. When heated past the lower critical solution temperature (LCST), pNIPAM gels enter a deswollen, polymer-rich phase, characterized by a small volume.…”

mentioning

confidence: 99%

Extreme thermodynamics with polymer gel tori: Harnessing thermodynamic instabilities to induce large-scale deformations

et al. 2018

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When a swollen, thermoresponsive polymer gel is heated in a solvent bath, it expels solvent and deswells. When this heating is slow, deswelling proceeds homogeneously, as observed in a toroid-shaped gel that changes volume while maintaining its toroidal shape. By contrast, if the gel is heated quickly, an impermeable layer of collapsed polymer forms and traps solvent within the gel, arresting the volume change. The ensuing evolution of the gel then happens at fixed volume, leading to phase separation and the development of inhomogeneous stress that deforms the toroidal shape. We observe that this stress can cause the torus to buckle out of the plane, via a mechanism analogous to the bending of bimetallic strips upon heating. Our results demonstrate that thermodynamic instabilities, i.e., phase transitions, can be used to actuate mechanical deformation in an extreme thermodynamics of materials.

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“…In Supplementary Movie 1 a recirculation phenomenon is observed, similar to the one reported by Nash et al . 26 . This is an indication of a collective or swarming-like motion.…”

Section: Resultsmentioning

confidence: 99%

Induced clustering of Escherichia coli by acoustic fields

Gutiérrez-Ramos

Hoyos

Ruiz‐Suárez

2018

Sci Rep

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Brownian or self-propelled particles in aqueous suspensions can be trapped by acoustic fields generated by piezoelectric transducers usually at frequencies in the megahertz. The obtained confinement allows the study of rich collective behaviours like clustering or spreading dynamics in microgravity-like conditions. The acoustic field induces the levitation of self-propelled particles and provides secondary lateral forces to capture them at nodal planes. Here, we give a step forward in the field of confined active matter, reporting levitation experiments of bacterial suspensions of Escherichia coli. Clustering of living bacteria is monitored as a function of time, where different behaviours are clearly distinguished. Upon the removal of the acoustic signal, bacteria rapidly spread, impelled by their own swimming. Nevertheless, long periods of confinement result in irreversible bacteria entanglements that could act as seeds for levitating bacterial aggregates.

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Biofilm formation in geometries with different surface curvature and oxygen availability

Cited by 56 publications

References 28 publications

Zosteric acid and salicylic acid bound to a low density polyethylene surface successfully control bacterial biofilm formation

Zosteric acid and salicylic acid bound to a low density polyethylene surface successfully control bacterial biofilm formation

Extreme thermodynamics with polymer gel tori: Harnessing thermodynamic instabilities to induce large-scale deformations

Induced clustering of Escherichia coli by acoustic fields

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