Biofilm formation by staphylococci in health-related environments and recent reports on their control using natural compounds

Yong, Yi Yi; Dykes, Gary A.; Choo, Wee Sim

doi:10.1080/1040841x.2019.1573802

Cited by 55 publications

(41 citation statements)

References 195 publications

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“…Virulence factors of these bacteria enable them to escape the host’s immune response and facilitate adhesion to host tissues and foreign materials [ 9 ]. One of the most important S. epidermidis virulence factors is extracellular secretion of mucus which facilitates bacterial adhesion to biomaterials, i.e., to the plastic materials used to manufacture medical devices, such as prostheses and catheters [ 10 ]. Microorganisms attached to solid surfaces and surrounded by self-produced extracellular polymeric substances (EPS) constitute structurally and functionally complex communities known as the biofilm.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Biofilm Formation and Prevalence of Multidrug-Resistant Strains of Staphylococcus epidermidis Isolated from Neonates with Sepsis in Southern Poland

et al. 2021

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Staphylococcus epidermidis strains play an important role in nosocomial infections, especially in the ones associated with biofilm formation on medical devices. The paper was aimed at analyzing the mechanisms of antibiotic resistance and confirming the biofilm-forming ability among S. epidermidis strains isolated from the blood of hospitalized newborns. Genetic analysis of resistance mechanism determinants included multiplex PCR detection of mecA, ermA, ermB, ermC, msrA, and mef genes. Biofilm analysis comprised phenotypic and genotypic methods including Christensen and Freeman methods and PCR detection of the icaADB gene complex. Among the tested S. epidermidis strains, 89% of the isolates were resistant to methicillin, 67%—to erythromycin, 53%—to clindamycin, 63%—to gentamicin, and 23%—to teicoplanin, while all the strains were susceptible to vancomycin and linezolid. The mecA gene was detected in 89% of the isolates, the ermC gene was the most common and present among 56% of the strains, while the msrA gene was observed in 11% isolates. Eighty-five percent of the strains were described as biofilm-positive by phenotypic methods and carried the icaADB gene cluster. Multidrug resistance and the biofilm-forming ability in most of the strains tested may contribute to antimicrobial therapy failure (p < 0.05).

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Section: Introductionmentioning

confidence: 99%

Evaluation of Biofilm Formation and Prevalence of Multidrug-Resistant Strains of Staphylococcus epidermidis Isolated from Neonates with Sepsis in Southern Poland

et al. 2021

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“…Staphylococci have been associated with a plethora of human medical issues, including localized skin infections, surgical site infections (SSIs), and medical device-related infections ( Yong et al, 2019 ). Notably, Staphylococcus pseudintermedius has appeared over the last decade as a critically significant bacterial pathogen responsible for skin, soft tissue, wound, and SSIs and possesses increasing zoonotic potential for cutaneous infections in humans ( Murayama et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Short-Chain Antimicrobial Peptides With Combined Antimicrobial and Anti-inflammatory Bioactivities for the Treatment of Zoonotic Skin Pathogens From Canines

Tang

Yang

et al. 2021

Front. Microbiol.

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The incidence of zoonotic Staphylococcus pseudintermedius and Microsporum canis infections is rapidly growing worldwide in the context of an increasing frequency of close contact between animals and humans, presenting challenges in both human and veterinary medicine. Moreover, the development of microbial resistance and emergence of recalcitrant biofilms, accompanied by the insufficiency of new antimicrobial agents, have become major obstacles in treating superficial skin infections caused by various microbes including S. pseudintermedius and M. canis. Over recent years, the prospects of antimicrobial peptides as emerging antimicrobials to combat microbial infections have been demonstrated. In our study, two novel short-chain peptides, namely, allomyrinasin and andricin B, produced by Allomyrina dichotoma and Andrias davidianus, were revealed to exhibit potent antimicrobial efficacy against clinical isolates of S. pseudintermedius and M. canis with remarkable and rapid fungicidal and bactericidal effects, while allomyrinasin exhibited inhibition of biofilm formation and eradication of mature biofilm. These peptides displayed synergistic activity when combined with amoxicillin and terbinafine against S. pseudintermedius and M. canis. Cytoplasmic leakage via cytomembrane permeabilization serves as a mechanism of action. Extremely low hemolytic activity and serum stability in vitro, as well as superior anti-infective efficacy in reducing bacterial counts and relieving the inflammatory response in vivo, were detected. The potent antibacterial, antifungal, and anti-inflammatory activities of allomyrinasin and andricin B might indicate promising anti-infective alternatives for the treatment of S. pseudintermedius and M. canis infections in the context of human and veterinary medicine.

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“…It has also been demonstrated that many types of infections can originate or progress from persistent staphylococci forms well known as biofilms [ 12 ]; Staphylococcus biofilms produced both by CoP and CoN strains are one of the leading causes of catheter or implant-associated infections [ 13 ], as well as more severe diseases due to the onset of antibiotic resistance, persistent inflammation and delays in healing [ 14 ]. Staphylococci in biofilm or planktonic state also produce specific volatile organic compounds (VOCs), causal agents of malodorous wounds.…”

Section: Introductionmentioning

confidence: 99%

Lactoferrin-Derived Peptides as a Control Strategy against Skinborne Staphylococcal Biofilms

et al. 2020

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Coagulase-negative staphylococci (CoNS) widely colonize the human skin and play an active role in host defense. However, these bacteria may cause malodours and increase infection incidence rate in immune-compromised patients and individuals with catheters and implants. CoNS spreading is favored by biofilm formation that also promotes the release of virulence factors and drug resistance. Biofilm control or eradication by antimicrobial peptides (AMPs) represents an attractive strategy which is worth investigating. In this work, bovine lactoferrin (BLF) hydrolysate (HLF) was in vitro evaluated for its antimicrobial and antibiofilm activities against skin-related coagulase negative and positive staphylococci. Despite a minimal inhibitory concentration (MIC) recorded for HLF ranging from 10 to more than 20 mg/mL, a minimal biofilm inhibitory concentration (MIBC) equal to 2.5 mg/mL was found for most target strains. Conversely, MIBC values referred to the individual peptides, LFcinB or LFmpin (herein purified and identified) were significantly lower. Finally, the application of 2.5 mg/mL HLF solution by dipping and spraying on biofilm-attached glass surfaces also caused a high biofilm eradication rate depending on the incubation time, thus attracting interest for future applications in cosmetic formulation for skin care.

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Biofilm formation by staphylococci in health-related environments and recent reports on their control using natural compounds

Cited by 55 publications

References 195 publications

Evaluation of Biofilm Formation and Prevalence of Multidrug-Resistant Strains of Staphylococcus epidermidis Isolated from Neonates with Sepsis in Southern Poland

Evaluation of Biofilm Formation and Prevalence of Multidrug-Resistant Strains of Staphylococcus epidermidis Isolated from Neonates with Sepsis in Southern Poland

Evaluation of Short-Chain Antimicrobial Peptides With Combined Antimicrobial and Anti-inflammatory Bioactivities for the Treatment of Zoonotic Skin Pathogens From Canines

Lactoferrin-Derived Peptides as a Control Strategy against Skinborne Staphylococcal Biofilms

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