Biofabrication of 3D constructs: fabrication technologies and spider silk proteins as bioinks

DeSimone, Elise; Schacht, Kristin; Jüngst, Tomasz; Groll, Jürgen; Scheibel, Thomas

doi:10.1515/pac-2015-0106

Cited by 55 publications

(42 citation statements)

References 82 publications

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“…By incorporating the cell adhesion peptide motif RGD they were able to increase cell adhesion and proliferation [28]. The same group showed that this silk biomaterial has enough mechanical strength to print larger, more intricate structures [34].…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Current developments in 3D bioprinting for tissue engineering

Cornelissen

Faulkner-Jones

Shu

2017

Current Opinion in Biomedical Engineering

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This version is available at https://strathprints.strath.ac.uk/61660/ Strathprints is designed to allow users to access the research output of the University of Strathclyde. Unless otherwise explicitly stated on the manuscript, Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Please check the manuscript for details of any other licences that may have been applied. You may not engage in further distribution of the material for any profitmaking activities or any commercial gain. You may freely distribute both the url (https://strathprints.strath.ac.uk/) and the content of this paper for research or private study, educational, or not-for-profit purposes without prior permission or charge.Any correspondence concerning this service should be sent to the This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPTCurrent developments in 3D bioprinting for tissue engineering AbstractThe field of 3-dimensional (3D) bioprinting have enjoyed rapid development in the past few years for the applications in tissue engineering and regenerative medicine. In this review, we summarize the most updated developments in 3D bioprinting for the applications in the tissue engineering with a focus on the printable biomaterials used as bioinks. These developments include 1) novel printing regimes have been enabled by the use of fugitive inks for the creation of intricate structures e.g. vascularized tissue constructs; 2) mechanical strength of printed constructs can be enhanced by co-printing soft and hard biomaterials; 3) bioprinted in-vitro models for drug testing applications are closer to reality. We conclude that the research and application of new bioinks will remain the key highlights of the future developments in 3D bioprinting for tissue engineering.

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Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Current developments in 3D bioprinting for tissue engineering

Cornelissen

Faulkner-Jones

Shu

2017

Current Opinion in Biomedical Engineering

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“…3,8) Die wichtigste physikalische Eigenschaft der Biotinte ist ihr Fließverhalten. 4,9) 4) Die fertigungsbedingten Anforderungen an Biotinten kollidieren oft mit den biologischen. Im gedruckten Konstrukt müssen eingekapselte Zellen wandern, sich teilen und differenzieren können.…”

Section: Biotintenunclassified

“…11) Zudem muss sich das Biomaterialgerüst ohne schädliche Abbauprodukte in einem Zeitraum zersetzen, in dem sich neues Gewebe bildet und das Gerüst ersetzt. 9)…”

Section: Biotintenunclassified

“…S Etablierte Biotinten enthalten natürliche Polymere wie Alginat oder Kollagen und synthetische Polymere wie Polyethylenglykol oder Polylactid sowie modifizierte Varianten und Kombinationen dieser Polymere. 3,9) Alginat ist ein wichtiger und häufig verwendeter Vertreter der Hydrogele. Es bildet eine zellfreundliche Umgebung, indem es die Zellen mit Nährstoffen und Sauerstoff versorgt.…”

Section: Biopolymere Für Den 3-d-druckunclassified

“…Noch zellfreundlicher als Kollagen ist seine modifizierte Variante, die Gelatine. 3,9) In 13,14) Vorteil von rekombinanten Spinnenseidenproteinen ist, dass sie sich in nanofibrilläre Netzwerke selbstassemblieren und außerdem mechanisch stabile Hydrogele bilden. Wissenschaftler nutzten diese Hydrogele bereits als zellfreundliche Biotinte in der Biofabrikation (Abbildung 3).…”

Section: Biopolymere Für Den 3-d-druckunclassified

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Zellgewebe aus dem Drucker

Scheibel¹,

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Boccaccını³

et al. 2016

Nachr Chem

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Silk‐Based Bioinks for 3D Bioprinting

Chawla

Midha

Sharma

et al. 2018

Adv Healthcare Materials

148

103

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3D bioprinting field is making remarkable progress; however, the development of critical sized engineered tissue construct is still a farfetched goal. Silk fibroin offers a promising choice for bioink material. Nature has imparted several unique structural features in silk protein to ensure spinnability by silkworms or spider. Researchers have modified the structure-property relationship by reverse engineering to further improve shear thinning behavior, high printability, cytocompatible gelation, and high structural fidelity. In this review, it is attempted to summarize the recent advancements made in the field of 3D bioprinting in context of two major sources of silk fibroin: silkworm silk and spider silk (native and recombinant). The challenges faced by current approaches in processing silk bioinks, cellular signaling pathways modulated by silk chemistry and secondary conformations, gaps in knowledge, and future directions acquired for pushing the field further toward clinic are further elaborated.

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Biofabrication of 3D constructs: fabrication technologies and spider silk proteins as bioinks

Cited by 55 publications

References 82 publications

Current developments in 3D bioprinting for tissue engineering

Current developments in 3D bioprinting for tissue engineering

Zellgewebe aus dem Drucker

Silk‐Based Bioinks for 3D Bioprinting

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