Bioethanol production from oil palm empty fruit bunch with SSF and SHF processes using Kluyveromyces marxianus yeast

Sukhang, Suwanan; Choojit, Saovanee; Reungpeerakul, Taweesak; Sangwichien, Chayanoot

doi:10.1007/s10570-019-02778-2

Cited by 38 publications

(22 citation statements)

References 47 publications

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“…Kluyveromyces marxianus is a new alternative yeast for wine and cider making that can ferment several carbohydrate substrates into ethanol and grow rapidly ( Fonseca et al, 2008 , Sukhang et al, 2019 , Yamahata et al, 2020 ). As an ethanologenic yeast, K. marxianus is one of the few species that can secrete endogenous polygalacturonase, an important pectolytic glucanase primarily implicated in the solubility of pectin ( Serrat, Rodriguez, Camacho, Vallejo, Ageitos, & Villa, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative metabolome and transcriptome analyses of the properties of Kluyveromyces marxianus and Saccharomyces yeasts in apple cider fermentation

Zhang

Lan

et al. 2022

Food Chemistry: Molecular Sciences

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Section: Introductionmentioning

confidence: 99%

Comparative metabolome and transcriptome analyses of the properties of Kluyveromyces marxianus and Saccharomyces yeasts in apple cider fermentation

Zhang

Lan

et al. 2022

Food Chemistry: Molecular Sciences

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“…In an SSF process in which yeast is grown simultaneously during hydrolysis (Fig. 2 a), this inhibition is prevented as the glucose is consumed to produce desired chemicals as it is released [ 21 , 22 ]. Because hydrolyzing cellulose in emulsions as opposed to microcrystals is advantageous at all temperatures tested between 30 and 50 °C (Fig.…”

Section: Resultsmentioning

confidence: 99%

Cellulosic biofuel production using emulsified simultaneous saccharification and fermentation (eSSF) with conventional and thermotolerant yeasts

Hoffman

Álvarez

Alfassi

et al. 2021

Biotechnol Biofuels

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Background Future expansion of corn-derived ethanol raises concerns of sustainability and competition with the food industry. Therefore, cellulosic biofuels derived from agricultural waste and dedicated energy crops are necessary. To date, slow and incomplete saccharification as well as high enzyme costs have hindered the economic viability of cellulosic biofuels, and while approaches like simultaneous saccharification and fermentation (SSF) and the use of thermotolerant microorganisms can enhance production, further improvements are needed. Cellulosic emulsions have been shown to enhance saccharification by increasing enzyme contact with cellulose fibers. In this study, we use these emulsions to develop an emulsified SSF (eSSF) process for rapid and efficient cellulosic biofuel production and make a direct three-way comparison of ethanol production between S. cerevisiae, O. polymorpha, and K. marxianus in glucose and cellulosic media at different temperatures. Results In this work, we show that cellulosic emulsions hydrolyze rapidly at temperatures tolerable to yeast, reaching up to 40-fold higher conversion in the first hour compared to microcrystalline cellulose (MCC). To evaluate suitable conditions for the eSSF process, we explored the upper temperature limits for the thermotolerant yeasts Kluyveromyces marxianus and Ogataea polymorpha, as well as Saccharomyces cerevisiae, and observed robust fermentation at up to 46, 50, and 42 °C for each yeast, respectively. We show that the eSSF process reaches high ethanol titers in short processing times, and produces close to theoretical yields at temperatures as low as 30 °C. Finally, we demonstrate the transferability of the eSSF technology to other products by producing the advanced biofuel isobutanol in a light-controlled eSSF using optogenetic regulators, resulting in up to fourfold higher titers relative to MCC SSF. Conclusions The eSSF process addresses the main challenges of cellulosic biofuel production by increasing saccharification rate at temperatures tolerable to yeast. The rapid hydrolysis of these emulsions at low temperatures permits fermentation using non-thermotolerant yeasts, short processing times, low enzyme loads, and makes it possible to extend the process to chemicals other than ethanol, such as isobutanol. This transferability establishes the eSSF process as a platform for the sustainable production of biofuels and chemicals as a whole.

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“…Although the traditional focus has been on cell factory design, the critical need to exploit second-generation biomasses to achieve sustainability is now a major driver of research. Indeed, this leads to somewhat of a paradigm shift since, in these scenarios, the substrate achieves equal importance to the product: a process that does not use residual (Fei et al, 2016), (B) (Pomraning et al, 2019), (C) (Liang et al, 2012), (D) (Gao et al, 2014), (E) (Chaiyaso et al, 2019), (F) (Kahr et al, 2015), (G) (Quarterman et al, 2017), (H) (Antil et al, 2015), (I) (Ben Atitallah et al, 2019), (J) (Mierzejewska et al, 2019), (K) (Yuan et al, 2017), (L) (Oberoi et al, 2012), (M) (Rodrigues et al, 2016), (N) (Sukhang et al, 2020), (O) (Camargo et al, 2014) and (P) (Saini et al, 2015). biomasses will struggle to deliver sustainability.…”

Section: Discussionmentioning

confidence: 99%

“…Two different and representative yeast metabolisms (lipidogenesis and alcoholic fermentation) are reported together with LCBs and enzymatic cocktails used in some selected examples. References: (A) (Fei et al ., 2016 ), (B) (Pomraning et al ., 2019 ), (C) (Liang et al ., 2012 ), (D) (Gao et al ., 2014 ), (E) (Chaiyaso et al ., 2019 ), (F) (Kahr et al ., 2015 ), (G) (Quarterman et al ., 2017 ), (H) (Antil et al ., 2015 ), (I) (Ben Atitallah et al ., 2019 ), (J) (Mierzejewska et al ., 2019 ), (K) (Yuan et al ., 2017 ), (L) (Oberoi et al ., 2012 ), (M) (Rodrigues et al ., 2016 ), (N) (Sukhang et al ., 2020 ), (O) (Camargo et al ., 2014 ) and (P) (Saini et al ., 2015 ).…”

Section: Combination Of Microbial Enzymes and Cell Factories In Lcbs‐...mentioning

confidence: 99%

Interdependence between lignocellulosic biomasses, enzymatic hydrolysis and yeast cell factories in biorefineries

Bertacchi

Jayaprakash

Morrissey

et al. 2021

Microbial Biotechnology

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Biorefineries have a pivotal role in the bioeconomy scenario for the transition from fossil-based processes towards more sustainable ones relying on renewable resources. Lignocellulose is a prominent feedstock since its abundance and relatively low cost. Microorganisms are often protagonists of biorefineries, as they contribute both to the enzymatic degradation of lignocellulose complex polymers and to the fermentative conversion of the hydrolyzed biomasses into fine and bulk chemicals. Enzymes have therefore become crucial for the development of sustainable biorefineries, being able to provide nutrients to cells from lignocellulose. Enzymatic hydrolysis can be performed by a portfolio of natural enzymes that degrade lignocellulose, often combined into cocktails. As enzymes can be deployed in different operative settings, such as separate hydrolysis and fermentation (SHF) or simultaneous saccharification and fermentation (SSF), their characteristics need to be combined with microbial ones to maximize the process. We therefore reviewed how the optimization of lignocellulose enzymatic hydrolysis can ameliorate bioethanol production when Saccharomyces cerevisiae is used as cell factory. Expanding beyond biofuels, enzymatic cocktail optimization can also be pivotal to unlock the potential of non-Saccharomyces yeasts, which, thanks to broader substrate utilization, inhibitor resistance and peculiar metabolism, can widen the array of feedstocks and products of biorefineries.

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Bioethanol production from oil palm empty fruit bunch with SSF and SHF processes using Kluyveromyces marxianus yeast

Cited by 38 publications

References 47 publications

Comparative metabolome and transcriptome analyses of the properties of Kluyveromyces marxianus and Saccharomyces yeasts in apple cider fermentation

Comparative metabolome and transcriptome analyses of the properties of Kluyveromyces marxianus and Saccharomyces yeasts in apple cider fermentation

Cellulosic biofuel production using emulsified simultaneous saccharification and fermentation (eSSF) with conventional and thermotolerant yeasts

Interdependence between lignocellulosic biomasses, enzymatic hydrolysis and yeast cell factories in biorefineries

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