2001
DOI: 10.1590/s0103-90162001000100029
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Bioensaio rápido de determinação da sensibilidade da acetolactato sintase (ALS) a herbicidas inibidores

Abstract: Foi avaliada a atividade da acetolactato sintase (ALS), em plantas resistentes e suscetíveis de B. pilosa e A. quitensis após a aplicação de herbicidas inibidores da ALS. O método baseia-se na utilização do ácido ciclopropanodicarboxílico (CPCA) para inibir a cetoácido reductoisomerase (KARI), enzima que catalisa a reação seguinte do acetolactato na cadeia de biossíntese dos aminoácidos valina, leucina e isoleucina, provocando assim, o acúmulo de acetolactato, que na presença de um ácido forte forma acetoína. … Show more

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“…Bioassays techniques are widely used and are standardized and approved for their sensitivity and detection of herbicides. Several bioassay procedures were reported by various workers for various herbicides [18,19].…”
Section: Introductionmentioning
confidence: 99%
“…Bioassays techniques are widely used and are standardized and approved for their sensitivity and detection of herbicides. Several bioassay procedures were reported by various workers for various herbicides [18,19].…”
Section: Introductionmentioning
confidence: 99%