Biodiversity in Mediterranean buffalo using two microsatellite multiplexes

Mitochondrial D-loop nucleotide sequences of Egyptian river buffalo: Variation and phylogeny studies

“…El-Kholy et al (2007) using three microsatellites concluded that all Egyptian buffalo belong to one population. However Elbeltagy et al (2008) in their study on the genetic diversity of Northern and Southern-Egypt buffalo populations using 15 microsatellites reported a significant differences in allelic distribution at 5 loci and they concluded that Southern-Egypt buffalo could be considered as distinct population from the Northern-Egypt buffalo.…”

“…These differences are not well defined to be relied upon as taxonomic classification. Recently Elbeltagy et al (2008) classified the Egyptian buffalo into Northern and Southern subpopulations. Few studies have been taken place to investigate the genetic variations in Egyptian buffalo using microsatellites analysis.…”

Genetic polymorphism of two genes associated with carcass trait in Egyptian buffaloes

“…The absence of molecular genetic variation in Egyptian buffaloes was previously reported by El-Kholy et al [15] and El-beltagy et al [14] who studied the genetic diversity of North-and South-Egyptian buffalo populations using microsatellites and reported that all Egyptian buffaloes belong to one population.…”

“…Depending on the alteration of the presence of C/T at position 4685 (within intron 14), it is easy to differentiate between 3 different genotypes: TT with undigested one fragment at 670-bp, CC with two digested fragments at 530-bp and 140-bp and TC with three fragments at 670-bp, 530-bp and 140-bp. All buffalo animals investigated in this study are genotyped as CC where all the tested buffalo DNA amplified fragments were digested with FokI endonuclease and gave two digested fragments at 530-and 140-bp.…”

“…These differences are not well defined to be relied on as taxonomic classification. Few studies [15,14] have investigated the genetic variations in Egyptian buffaloes and concluded that all Egyptian river buffaloes belong to one population (B. bubalis bubalis).…”