Biodiversity and antimicrobial potential of bacterial endophytes from halophyte Salicornia brachiata

Abstract

Untapped natural habitats like halophytes, marsh land, and marine environment are suitable arena for chemical ecology between plants and microbes having environmental impact. Endophytes constitute an ecofriendly option for the promotion of plant growth and to serve as sustainable resources of novel bioactive natural products. The present study focusing on biodiversity of bacterial endophytes from Salicornia brachiata, led to isolation of around 350 bacterial endophytes. Phylogenetic analysis of 63 endophytes revealed 13 genera with 29 different species, belonging to 3 major groups: Firmicutes, Proteobacteria and Actinobacteria. 30% isolates belonging to various genera demonstrated broadspectrum antibacterial and antifungal activities against a panel of human, plant, and aquatic infectious agents. An endophytic isolate Bacillus amyloliquefaciens 5NPA-1, exhibited strong in-vitro antibacterial activity against human pathogen S. aureus and phytopathogen X. campestris. Investigation through LC-MS/MS-based molecular networking and bioactivity-guided purification led to the identification of three bioactive compounds belonging to lipopeptide class on the basis of 1 H-and 13 C-NMR and MS analysis. To our knowledge, this is the first report studying bacterial endophytic biodiversity of Salicornia brachiata and isolation of bioactive compounds from its endophyte. Overall, the present study provides insights into the diversity of endophytes associated with the plants from the extreme environment as rich source of metabolites with remarkable agricultural applications and therapeutic properties.

Facile Alternative Sustainable Process for the Selective Extraction of Microbial Melanin

“…was collected from Victor Port, Amreli district, Gujarat, on February 26, 2018 (N 20°58′53.2″, E 071°33′21.2″). The collected plant sample was packed in sterile Ziploc bags and brought to the laboratory in an ice-box for immediate processing as per the reported method. , Briefly, the sample was cleaned and washed with water and Tween 20 (0.1% v/v) followed by surface sterilization sequentially with 4% NaOCl, 2.5% Na 2 S 2 O 3 , 70% ethyl alcohol, and sterile distilled water. The plant sample was subjected to continuous drying at 100 °C for 15 min and then plant parts were cut into small pieces and approximately 2 g of the plant material was macerated in 1× PBS (pH 7).…”

Exploring the Promise of Endophytic Fungi: A Review of Novel Antimicrobial Compounds

“…Firstly, plants that grow in unique environments, particularly if those plants have unusual biology or a novel survival strategy. For example, Singh et al (2020) [ 66 ] isolated the endophytic bacterium Bacillus amyloliquefaciens 5NPA-1 from Salicornia brachiata , a halophytic plant that grows in salty marsh lands, collected from the Gujarat coast, India. Antimicrobial screening of the B. amyloliquefaciens 5NPA-1 extract revealed strong inhibitory action against the human pathogen Staphylococcus aureus and the phytopathogen Xanthomonas campestris [ 66 ].…”

“…For example, Singh et al (2020) [ 66 ] isolated the endophytic bacterium Bacillus amyloliquefaciens 5NPA-1 from Salicornia brachiata , a halophytic plant that grows in salty marsh lands, collected from the Gujarat coast, India. Antimicrobial screening of the B. amyloliquefaciens 5NPA-1 extract revealed strong inhibitory action against the human pathogen Staphylococcus aureus and the phytopathogen Xanthomonas campestris [ 66 ]. Secondly, plants that are endemic to a region, especially in areas which were once part of an ancient land mass or those with an unusually long lifespan, can be considered.…”

Selective Partition of Lipopeptides from Fermentation Broth: A Green and Sustainable Approach

“…and Pseudomonas sp. are known to exhibit remarkable antimicrobial properties as well as inhibitory capacities against mosquitoes Anopheles stephensi, Aedes aegypti, and Culex sp. that are vectors for various deadly diseases. − Therefore, the extracted lipopeptides in the PPG layer were further assessed for their antimicrobial and mosquitocidal properties along with plant toxicity.…”