Biodistribution and tumour localization of radiolabelled monoclonal antibody during continuous infusion in nude mice and with human tumour xenografts

An anti‐doxorubicin monoclonal antibody modulates kinetic and dynamic characteristics of the drug

“…The increased liver and spleen drug uptake in the animals pre-treated with the MAb could reflect the general biodistribution of MAbs, as shown in numerous experiments of radiolabelled localization (Pimm et al, 1987).…”

An anti‐doxorubicin monoclonal antibody modulates kinetic and dynamic characteristics of the drug

“…The increased liver and spleen drug uptake in the animals pre-treated with the MAb could reflect the general biodistribution of MAbs, as shown in numerous experiments of radiolabelled localization (Pimm et al, 1987).…”

Differences between the catabolism and tumour distribution of intact monoclonal antibody (791T/36) and its Fab/c fragment in mice with tumour xenografts revealed by the use of a residualizing radiolabel (dilactitol-125I-tyramine) and autoradiography

“…At determined times from 2 min to 3 days after injection, 20 gl blood samples were drawn from the tail vein using heparinized micropipettes (Drummond Microcaps, Drummond, Broomhill, Pa., USA) and radioactivity was measured. The 2-min blood sample was used to determine the injected dose using calculated blood volume (11.2% body weight) [16]. The recovered dose was typically at least 90% of that expected.…”

Carrier design: biodistribution of branched polypeptides with a poly(L-lysine) backbone

“…The 125I-labeled polypeptide was purified on a G-25 Sephadex gel column using 0.066 M phosphate buffer (pH 7.6) containing 0.25% gelatin as eluent. Electrophoresis on native polyacrylamide gel with a continuous 8-25% gradient (PhastGel gradient [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] Pharmacia-LKB, Uppsala, Sweden) was applied to assess the low molecular weight labeled product content of the preparation.…”

“…The blood samples, visceral organs, and residual carcasses were weighed and assayed for radioactivity in a conventional 7-counter. Results of the bloodclearance study were expressed as a percentage of the zerotime count rate assuming the blood volume of the mouse (mL) to be 11.2 % of the body weight (g) (23). Area under the blood concentration-time curve up to 6 h following injection was calculated by the trapezoidal rule (24).…”

An anti‐doxorubicin monoclonal antibody modulates kinetic and dynamic characteristics of the drug

“…The increased liver and spleen drug uptake in the animals pre-treated with the MAb could reflect the general biodistribution of MAbs, as shown in numerous experiments of radiolabelled localization (Pimm et al, 1987).…”

Differences between the catabolism and tumour distribution of intact monoclonal antibody (791T/36) and its Fab/c fragment in mice with tumour xenografts revealed by the use of a residualizing radiolabel (dilactitol-125I-tyramine) and autoradiography

“…At determined times from 2 min to 3 days after injection, 20 gl blood samples were drawn from the tail vein using heparinized micropipettes (Drummond Microcaps, Drummond, Broomhill, Pa., USA) and radioactivity was measured. The 2-min blood sample was used to determine the injected dose using calculated blood volume (11.2% body weight) [16]. The recovered dose was typically at least 90% of that expected.…”

Carrier design: biodistribution of branched polypeptides with a poly(L-lysine) backbone

“…The 125I-labeled polypeptide was purified on a G-25 Sephadex gel column using 0.066 M phosphate buffer (pH 7.6) containing 0.25% gelatin as eluent. Electrophoresis on native polyacrylamide gel with a continuous 8-25% gradient (PhastGel gradient [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] Pharmacia-LKB, Uppsala, Sweden) was applied to assess the low molecular weight labeled product content of the preparation.…”

“…The blood samples, visceral organs, and residual carcasses were weighed and assayed for radioactivity in a conventional 7-counter. Results of the bloodclearance study were expressed as a percentage of the zerotime count rate assuming the blood volume of the mouse (mL) to be 11.2 % of the body weight (g) (23). Area under the blood concentration-time curve up to 6 h following injection was calculated by the trapezoidal rule (24).…”

An anti‐doxorubicin monoclonal antibody modulates kinetic and dynamic characteristics of the drug

“…The increased liver and spleen drug uptake in the animals pre-treated with the MAb could reflect the general biodistribution of MAbs, as shown in numerous experiments of radiolabelled localization (Pimm et al, 1987).…”

Differences between the catabolism and tumour distribution of intact monoclonal antibody (791T/36) and its Fab/c fragment in mice with tumour xenografts revealed by the use of a residualizing radiolabel (dilactitol-125I-tyramine) and autoradiography

“…At determined times from 2 min to 3 days after injection, 20 gl blood samples were drawn from the tail vein using heparinized micropipettes (Drummond Microcaps, Drummond, Broomhill, Pa., USA) and radioactivity was measured. The 2-min blood sample was used to determine the injected dose using calculated blood volume (11.2% body weight) [16]. The recovered dose was typically at least 90% of that expected.…”

Carrier design: biodistribution of branched polypeptides with a poly(L-lysine) backbone

“…The 125I-labeled polypeptide was purified on a G-25 Sephadex gel column using 0.066 M phosphate buffer (pH 7.6) containing 0.25% gelatin as eluent. Electrophoresis on native polyacrylamide gel with a continuous 8-25% gradient (PhastGel gradient [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] Pharmacia-LKB, Uppsala, Sweden) was applied to assess the low molecular weight labeled product content of the preparation.…”

“…The blood samples, visceral organs, and residual carcasses were weighed and assayed for radioactivity in a conventional 7-counter. Results of the bloodclearance study were expressed as a percentage of the zerotime count rate assuming the blood volume of the mouse (mL) to be 11.2 % of the body weight (g) (23). Area under the blood concentration-time curve up to 6 h following injection was calculated by the trapezoidal rule (24).…”