Biodiesel production and nutrient removal by microalgae cultured on poultry waste

Abstract

Microalgae are a potential feedstock for a vast renewable products and different applications in biotechnology. This investigation of using poultry waste extracts for growing Anabaena, Chlorella and mixed culture of both showed that 5 g/L poultry waste extract was the most probable concentration for enhancing growth and lipid production. Percentage increase in lipid content over control (BBM) was (94.2, 56.4 and 28.8 %), biomass productivity was (31.61, 51.86 and 42.04 mg/L/day) and lipid productivity was (4.59, 10.53 and 6.94 mg/L/day) for Anabaena, Chlorella and mixed culture respectively. The role of microalgae in bioremediation of poultry waste was effective, maximum reduction percentage of NH 3 was (84.07%) whereas it was (80.41%) for phosphorus. Microwave pretreatment of harvested cells increased the lipid content to 17.6% in Anabaena, 31% in Chlorella and 23% for mixture. Lipid extraction, esterification, GC-Ms analysis for fatty acid profile, showed that saturated fatty acids in Chlorella and algal mixture were higher than their corresponding controls. Cetane number of the produced biodiesel was ranged from (53.7 to 56.4) while iodine value was ranged from (63.5 to 88.4). This study revealed that poultry waste extract can be used as a promising ecofriendly microalgae medium for high production of biomass and lipid which in turn produce high quality biodiesel meet the international standards biodiesel.

Biotreatment of Poultry Waste Coupled with Biodiesel Production Using Suspended and Attached Growth Microalgal-Based Systems

“…Similar lipid contents of Leptolyngbya sp.-based cultures have also been recorded in suspended (6.3-14.8% d.w.) [39] and attached systems (9.8-24.8% d.w.) [27,61]. Shabana et al [7] recorded lipid content of 20.3% d.w., by cultivating Chlorella vulgaris in poultry waste extract with laboratory-scale experiments. The lipid contents achieved in the present study were lower than those recorded in previous research works applying poultry waste (Table S1), although it is worth mentioning that most of the substrates used in the literature had undergone some form of pretreatment.…”

“…Only a few studies have used PL as growth substrate for the cultivation of microalgae or cyanobacteria (Table S1). Additionally, limited research has been performed on PL extract (PLE) derived after sun drying, blending, and soaking the poultry faeces [7][8][9][10][11][12]. According to Table S1, pretreatment methods of PL including sterilization, centrifugation, dilution, or hydrolization are frequently applied to produce more favorable conditions that promote algal growth.…”