Biodegradation of toxic dyes: a comparative study of enzyme action in a microbial system

Pande, Veni; Pandey, Satish Chandra; Joshi, Tushar; Sati, Diksha; Gangola, Saurabh; Kumar, Saurabh; Samant, Mukesh

doi:10.1016/b978-0-12-818307-6.00014-7

Cited by 18 publications

(6 citation statements)

References 132 publications

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“…Each test tube contains 15 mL of the nutrient broth and different concentrations of the selected dye (100, 80, 60, 40 and 20 ppm). After 3 days of incubation the culture tubes mixture was centrifuged at 5000 rpm for 20 min and the remaining concentration of dye in supernatant was determined through UV-Visible spectrophotometer at 467 nm [15] The decolorization of the dye were carried out in test tubes having a capacity of 20 mL containing 15 mL of nutrient broth and dye solution. The nutrient broth containing Brown 706 was autoclaved at 121 • C for 15 min to avoid contamination by unwanted bacteria.…”

Section: Effects Of Dye Concentration On Dye Degradabilitymentioning

confidence: 99%

Section: Optimization Of the Physicochemical Parameters For The Optim...mentioning

confidence: 99%

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Biodegradation of Brown 706 Dye by Bacterial Strain Pseudomonas aeruginosa

Khan

Rehman

Zahoor

et al. 2021

Water

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Dyes are the most challenging pollutants for the aquatic environment that are not only toxic, but also interfering photosynthesis as light penetration into deep water is changed. A number of methods are used for the water reclamation, however, among them biological methods are preferably used due to their compatibility with nature. In the present research, 15 different bacterial strains were used to decolorize Brown 706 dye. Among the bacterial strains, Pseudomonas aeruginosa showed maximum decolorization activity; hence in the subsequent experiments Pseudomonas aeruginosa was used. First the decolorization activities were carried out under different physicochemical conditions to obtain the optimum decolorization benefits of the selected microorganism. The optimum conditions established were 37°C, pH of 7 and operation cycle time 72 h. In the subsequent experiment all optimum conditions were combined in a single experiment where 73.91% of decolorization efficiency was achieved. For the evaluation of metabolites formed after decolorization/degradation the aliquots containing bacteria were homogenized, filtered and then subjected to extraction. The extracted metabolites were then subjected to the silica gel column isolation. UV–Vis, FTIR, and NMR techniques were used to elucidate structures of the metabolites. Out of the collected metabolites only P-xylene was identified, which has been formed by cleavage of azo linkage by azo reductase enzyme of bacteria following the deamination and methylation of nitro substituted benzene ring.

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Section: Effects Of Dye Concentration On Dye Degradabilitymentioning

confidence: 99%

Section: Optimization Of the Physicochemical Parameters For The Optim...mentioning

confidence: 99%

Biodegradation of Brown 706 Dye by Bacterial Strain Pseudomonas aeruginosa

Khan

Rehman

Zahoor

et al. 2021

Water

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“…The culture mixture including the products generated from the degraded dairy e uent was spun in a centrifuge machine at room temperature for 10 minutes at 10,000 rpm after every 12 hours. An UV visible spectrophotometer was used to determine the absorbance value of the extracted supernatant mixture at 600 nm following centrifugation (Veni et al, 2019).…”

Section: Effect Of Inoculum Concentrationmentioning

confidence: 99%

Treatment of Dairy Effluent by Novel Bacteria

Ponraj,

Sriniva,

Ibrahim

2023

Preprint

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The main cause of river contamination is the dairy sector. Sludge and effluents from food-related businesses, particularly those in the dairy sector, contain a sizable amount of organic matter, fatty acids, and nitrogenous chemicals. Whey is the primary pollutant in milk processing effluent due to its enormous volume and high organic content. Between 85% and 95% of the milk's weight and 55% of its constituent parts are made up of it. Lactose makes up the majority of the 4-5% of carbs in whey. In this work, the biological approach was used to identify Enterobacter cloacae and Aeromonas intestinalis as Dairy-degrading bacteria. The analysis of optimizing several parameters for the degradation of dairy effluent. Instrument Analysis also confirmed the Structural Changes of Degradation in Dairy effluent.

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“…After a three-day incubation period, the degraded mixture was centrifuged at 10,000 rpm for 10 min in a centrifuge machine (SelectSpin Spectra 6C) at room temperature. The absorbance of the extracted supernatant was determined using a UV-Visible spectrophotometer (UV-1800 ENG.SOFT) [78].…”

Section: Impact Of Orange II Concentration On Degradationmentioning

confidence: 99%

Bacillus subtilis: As an Efficient Bacterial Strain for the Reclamation of Water Loaded with Textile Azo Dye, Orange II

Ikram

Naeem

Zahoor

et al. 2022

IJMS

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The azo dye orange II is used extensively in the textile sector for coloring fabrics. High concentrations of it are released into aqueous environments through textile effluents. Therefore, its removal from textile wastewater and effluents is necessary. Herein, initially, we tested 11 bacterial strains for their capabilities in the degradation of orange II dye. It was revealed in the preliminary data that B. subtilis can more potently degrade the selected dye, which was thus used in the subsequent experiments. To achieve maximum decolorization, the experimental conditions were optimized whereby maximum degradation was achieved at: a 25 ppm dye concentration, pH 7, a temperature of 35 °C, a 1000 mg/L concentration of glucose, a 1000 mg/L urea concentration, a 666.66 mg/L NaCl concentration, an incubation period of 3 days, and with hydroquinone as a redox mediator at a concentration of 66.66 mg/L. The effects of the interaction of the operational factors were further confirmed using response surface methodology, which revealed that at optimum conditions of pH 6.45, a dye concentration of 17.07 mg/L, and an incubation time of 9.96 h at 45.38 °C, the maximum degradation of orange II can be obtained at a desirability coefficient of 1, estimated using the central composite design (CCD). To understand the underlying principles of degradation of the metabolites in the aliquot mixture at the optimized condition, the study steps were extracted and analyzed using GC-MS(Gas Chromatography Mass Spectrometry), FTIR(Fourier Transform Infrared Spectroscopy), 1H and carbon 13 NMR(Nuclear Magnetic Resonance Spectroscopy). The GC-MS pattern revealed that the original dye was degraded into o-xylene and naphthalene. Naphthalene was even obtained in a pure state through silica gel column isolation and confirmed using 1H and 13C NMR spectroscopic analysis. Phytotoxicity tests on Vigna radiata were also conducted and the results confirmed that the dye metabolites were less toxic than the parent dye. These results emphasize that B. subtilis should be used as a potential strain for the bioremediation of textile effluents containing orange II and other toxic azo dyes.

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Biodegradation of toxic dyes: a comparative study of enzyme action in a microbial system

Cited by 18 publications

References 132 publications

Biodegradation of Brown 706 Dye by Bacterial Strain Pseudomonas aeruginosa

Biodegradation of Brown 706 Dye by Bacterial Strain Pseudomonas aeruginosa

Treatment of Dairy Effluent by Novel Bacteria

Bacillus subtilis: As an Efficient Bacterial Strain for the Reclamation of Water Loaded with Textile Azo Dye, Orange II

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