Biodegradation of Petroleum Compound Using Pseudomonas aeruginosa

Abstract

The present study was conducted to determine the biodegradation of petrol by Pseudomonas aeruginosa isolated from petrol contaminated soil. The isolated strain was able to grow in minimal broth along with 2.5%, 5%, 7.5% and 10% concentrations of petrol which indicated the capability of the organism in degrading petrol and utilizing it as a source for growth. The isolated strain's efficiency was determined by analyzing the parameters pH, optical density and CO2 released during petrol degradation. High Performance Liquid Chromatography (HPLC) analysis also confirmed the degradation of petrol by Pseudomonas aeruginosa.

Bakteri Indigen Pendegradasi Hidrokarbon Minyak Bumi di Kabupaten Siak Provinsi Riau

“…Mikroba yang dapat digunakan salah satunya yakni mikroba indigen yang merupakan biota lokal berasal dari lingkungan yang tercemar. Studi tentang biodegradasi hidrokarbon memanfaatkan bakteri indigen telah banyak dilakukan salah satunya di India menggunakan Pseudomonas aeruginosa [4]. Bakteri indigen memiliki kemampuan mendegradasi senyawa hidrokarbon untuk keperluan metabolismenya dan sudah teradaptasi dengan berbagai faktor lingkungan di habitat asalnya.…”

Assessing Bioremediation of Soils Polluted with Fuel Oil 6 by Means of Diffuse Reflectance Spectroscopy

“…The PAHs are dangerous and persistent FO6 compounds that can put people's health at risk and cause harm to the environment. Alkenes, in general, have toxic, carcinogenic, and mutagenic properties and represent a significant danger to health and the environment [6]. Inoculation of the growth medium: One gram of contaminated soil was mixed with 9 mL of sodium salt solution (NaCl 1%) to obtain a 10 −1 dilution, and it was vortex shaken for 1 min.…”

“…In this process, FO6 is a rich source of organic compounds that serve as nutrients for the growth and development of P. aeruginosa. Darsa and Thatheyus [6] reported that P. aeruginosa exhibits an excellent ability to degrade n-hexadecane (C 16 H 34 ). Das and Chandran [25] reported that P. aeruginosa in its catabolic cycle produces a biosurfactant called rhamnolipid, an enzyme considered biosurfactant that allows it to take the carbon from the hydrocarbon.…”

“…Bacteria are the most active agents in petroleum biodegradation. Nearly a hundred species of bacteria, representing thirty microbial genera, have been found to degrade petroleum products [4][5][6]. One of the most consistently isolated hydrocarbon-degrading bacteria from soil is Pseudomonas [7].…”

“…In this process, FO6 is a rich source of organic compounds that serve as nutrients for the growth and development of P. aeruginosa. Darsa and Thatheyus [6] reported that P. aeruginosa exhibits an excellent ability to degrade n-hexadecane (C16H34). Das and Chandran [25] Funding: This research was funded by UNIVERSIDAD NACIONAL DE CHIMBORAZO, Riobamba Ecuador, through the project "Soil organic carbon and sequestration in Ecuadorian paramo ecosystem"…”