Biodegradation of Monochloroacetic Acid by a Presumptive Pseudomonas sp. Strain R1 Bacterium Isolated from Malaysian Paddy (Rice) Field

Ismail, Siti Nurul Fasehah; Taha, Ahmed; Jing, Ng Hong; Wahab, Roswanira Ab.; Hamid, A. A. A.; Pakingking, Rolando V.; Huyop, Fahrul

doi:10.3923/biotech.2008.481.486

Cited by 14 publications

(4 citation statements)

References 12 publications

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“…TW1 [63], Xantobacter autotrophicus GJ10 [64,65], and Pseudomonas sp. R1 [66] are examples of bacteria that can degrade MCA haloacetates. Additionally, Meusel and Rehm [67] described Xanthobacter autotrophicus GJ10 that can degrade DCA.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of a Novel Bacterium from the Marine Environment for Trichloroacetic Acid Bioremediation

et al. 2020

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Halogenated compounds are an important class of environmental pollutants that are widely used in industrial chemicals such as solvents, herbicides, and pesticides. Many studies have been carried out to explore the biodegradation of these chemicals. Trichloroacetic acid (TCA) is one of the main halogenated compounds that are carcinogenic to humans and animals. The bacterium was isolated from the northern coastline of Johor Strait. In this study, the ability of strain MH2 to biodegrade TCA was evaluated by a growth experiment and dehalogenase enzyme assay. The growth profile of the isolated strain was examined. The doubling time for L. boronitolerans MH2 was found to be 32 h. The release of chloride ion in the degradation process was measured at 0.33 × 10−3 ± 0.03 mol∙L−1 after 96 h when the growth curve had reached its maximum within the late bacterial exponential phase. The results showed that the strain had a promising ability to degrade TCA by producing dehalogenase enzyme when cell-free extracts were prepared from growth on TCA as the sole carbon source with enzyme-specific activity, 1.1 ± 0.05 µmolCl−min−1∙mg−1 protein. Furthermore, the morphological, and biochemical aspects of the isolated bacterium were studied to identify and characterize the strain. The morphological observation of the isolated bacterium was seen to be a rod-shaped, Gram-positive, motile, heterotrophic, and spore-forming bacterium. The amplification of the 16S rRNA and gene analysis results indicated that the isolated bacterium had 98% similarity to Lysinibacillus boronitolerans. The morphological and biochemical tests supported the 16S rRNA gene amplification. To the best of the authors’ knowledge, this is the first reported case of this genus of bacteria to degrade this type of halogenated compound.

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Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of a Novel Bacterium from the Marine Environment for Trichloroacetic Acid Bioremediation

et al. 2020

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“…It was hypothesise that this bacterium produces dehalogenases to convert 2,2-DCP into pyruvate [26]. Besides 2,2-DCP, degradation of many other halogenated aliphatic acids, such as a monochloroacetic acid (MCA) [27], trichloroacetic acid (TCA) [28], and D,L2-chloropropionic acid (D,L-2CP) [29] were also reported. Most halogenated compounds are major environmental pollutants and was already been proven by numerous studies [30].…”

Section: Isolation and Morphological Analysis Of A Bacterium On 10 Mm...mentioning

confidence: 99%

Characterisation of a Bacterium from Selat Tebrau and Screening of Microbial Genomes for Dehalogenases

Oyewusi

Mohamed

Huyop

2022

J.Trop.Life.Science

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Current study was to investigate the presence of dehalogenase in the isolated bacterium from Selat Tebrau that can grow on 2,2-dichloropropionic acid (2,2-DCP is an active compound in herbicide Dalapon®). Strain RN1, a Gram-negative and rod in shape was tentatively identified as Enterobacter cancerogenus based on basic biochemical and the 16S rRNA gene analyses. The calculated cells doubling time were 5.29 hours based on growth of the bacterium in liquid minimal media with 10, 20 and 30 mM of 2,2-DCP, respectively. However, no growth was observed at 40 mM 2,2-DCP liquid minimal medium due to increase in 2,2-DCP toxicity. It was hypothesized that, strain RN1 produced dehalogenase(s) which merits a further study of the genomic data of the same genus and species available in the database. A putative dehalogenase, designated as DehRN was located in the published data of Enterobacter cancerogenus. Pairwise of DehRN amino acids with known dehalogenase resulted in sequence identity of <20%, suggesting a new class of dehalogenase enzyme in the Enterobacter cancerogenus.Keywords: 2,2-DCP, dehalogenase, dichloropropionate, Enterobacter, haloalkanoic acid

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“…It was also reported that MCA dehalogenase activity was found to be inducible, either in wild type of Pseudomonas sp. R1 (Ismail et al, 2008) or in its recombinant clone of Burkholderia spesies MBA4 (Su et al, 2013). Based on our result, we chose 1 mM of MCA concentration for initial optimization of inducer (IPTG) concentration.…”

Section: Growth Of E Coli Bl21 (De3)/pet-bcfd1 In Mca Containing Mediummentioning

confidence: 99%

Optimization of Monochloroacetic Acid Biodegradation by Recombinant E. coli BL21 (DE3)/pET-bcfd1 Carrying Haloacid Dehalogenase Gene from Bacillus cereus IndB1

Ratnaningsih

Ade

Putri

et al. 2021

Int. J. Renew. Energy Dev.

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In recent years, attention to microbial dehalogenase has continually increased due to its potential application, both in bioremediation and in the biosynthesis of fine chemicals. Many microbial recombinant strains carrying dehalogenase gene have been developed, particularly to increase the dehalogenase production and its quality. In this study, we aimed to find the optimum condition for the production of active haloacid dehalogenase by E. coli BL21 (DE3) harboring recombinant plasmid pET-bcfd1 that carried haloacid dehalogenase gene from Bacillus cereus IndB1 local strain. This would be examined by assessing the ability of whole cell life culture to degrade monochloroacetic acid (MCA) and quantifying the chloride ion released into the medium. Several variables were evaluated to find this optimal condition. We found that the best condition for MCA biodegradation using this recombinant clone was at 0.2 mM MCA, 10 μM of isopropyl β-D-1-thiogalactopyranoside (IPTG), 6 hours of pre-induction incubation at 37ºC with shaking, 2 hours IPTG induction at 30ºC with shaking, at pH 7 in Luria Bertani (LB) liquid medium without NaCl, which produced about 0.056 mM chloride ions. Inducer concentration, pre-induction incubation time and temperature, as well as induction time and temperature were apparent to be associated with the expression of the protein, while the MCA concentration and the pH of the medium influenced the ability of the recombinant E. coli BL21 (DE3)/pET-bcfd1 to grow in toxic environment. Our findings laid the foundation for exploration of dehalogenases from local Bacillus strains through genetic engineering for MCA biodegradation

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Biodegradation of Monochloroacetic Acid by a Presumptive Pseudomonas sp. Strain R1 Bacterium Isolated from Malaysian Paddy (Rice) Field

Cited by 14 publications

References 12 publications

Isolation and Characterization of a Novel Bacterium from the Marine Environment for Trichloroacetic Acid Bioremediation

Isolation and Characterization of a Novel Bacterium from the Marine Environment for Trichloroacetic Acid Bioremediation

Characterisation of a Bacterium from Selat Tebrau and Screening of Microbial Genomes for Dehalogenases

Optimization of Monochloroacetic Acid Biodegradation by Recombinant E. coli BL21 (DE3)/pET-bcfd1 Carrying Haloacid Dehalogenase Gene from Bacillus cereus IndB1

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