Biodegradation of crystal violet by an isolatedBacillus sp.

Abstract

-Synthetic dyes are widely used in the textile, cosmetic, printing, drug, and food processing industries. Triphenylmethane dyes belong to the most important group of synthetic dyes. They are generally considered as the xenobiotic compounds, which are very recalcitrant to biodegradation. Bacillus sp., was isolated from the treatment plant effluent of a textile and dyeing industry (SITEX) located in Ksar Hellal, Tunisia, decolorizes crystal violet (500 ppm) within 2.5 h under shaking condition at pH 7 and temperature 30 °C. The effect of dye concentration, temperature and initial pH of the solution were studied. The results obtained from the batch experiments revealed the ability of bacteria in removing dye. UV-Vis spectroscopy and FTIR analysis of samples before and after dye decolorization in culture medium confirmed decolorization of crystal violet. The phytotoxicity and microbial toxicity studies of extracted metabolites suggest the less toxic nature of them.

Use of active consortia of constructed ternary bacterial cultures via mixture design for Congo Red decolorization enhancement

“…The initial absorbance of the dye was used as a reference. The decolorization and COD removal were calculated according to the following equations [17,19,20]:…”

“…FTIR analysis was carried out using Perkin Elmer 783 Spectrophotometer (Nicolet Analytical Instruments, Madison, WI) and changes in % transmission at different wavelengths were observed. The samples were mixed with spectroscopically pure KBr in the ratio 2:200, pellets were fixed in the sample and the analysis was carried out [19,20].…”

“…Tests were carried out according to the ISO (1993) [25] on two kinds of seeds: Sorghum bicolor and Triticum aestivum. The microbial toxicity was carried out using S. paucimobilis on Mueller and Hinton agar plate having composition 1% peptone, 0.5% NaCl, 0.3% yeast extract and 2.5% agar [19].…”

Screening of Isolated Marine Bacteria for Multiple Biotechnological Applications

“…Tubes were incubated at 30°C for 48 hrs after which they were visually observed for decolorization if any. In addition, the growth of bacteria in the presence of dye was estimated by taking absorbance of the culture broth at 600 nm in Elico UV Vis Spectrophotometer (Ayed et al, 2009). …”

Biodegradation of Basic Violet 3 by Candida krusei isolated from textile wastewater

“…There are reports of BV biodegradation by bacteria (Sani and Banerjee 1999;Chen et al 2008a, b;Jang et al 2005;Ayed et al 2009) and Fungi (Bumpus and Brock 1988;Azmi et al 1998). Information is scanty regarding the use of yeast as potential degrader of BV.…”