BIODEGRADASI SLUDGE MINYAK BUMI DALAM SKALA MIKROKOSMOS: Simulasi Sederhana Sebagai Kajian Awal Bioremediasi Land Treatment

Abstract

Telah dilakukan penelitian skala mikrokosmos yang bertujuan untuk melakukan uji kemampuan konsorsium mikroba hidrokarbonoklastik untuk meningkatkan degradasi sludge minyak bumi dengan perlakuan penambahan pupuk NPK sebagai sumber nitrogen. Percobaan secara aerobik dilakukan dengan meletakkan erlenmeyer pada shaker incubator dengan kecepatan putaran 120 rpm pada temperatur 50°C. Pengamatan selama 150 hari pada skala mikrokosmos menunjukkan konsorsium mampu tumbuh dengan baik hingga beban sludge mencapai 50% (v/v). Pertumbuhan maksimum dan laju pertumbuhan maksimum konsorsium dalam medium cair terjadi pada perlakuan III C (penambahan 50% (v/v) substrat dalam bentuk sludge minyak bumi dan penambahan nitrogen dalam bentuk pupuk NPK sebanyak 30% (b/v) dan substrat yang ditambahkan. Hingga hari ke-150 semua perlakuan terdegradasi di atas 64%. Degradasi tertinggi terjadi pada perlakuan III A diikuti oleh perlakuan III C, masing-masing sebesar 88,72% dan 87,19%. Hasil analisis kromatografi gas menunjukkan bahwa pada t 15 dan t 30 muncul senyawa C 8 dan C 9 yang kemudian menghilang kembali setelah hari ke-30. Senyawa C 10 meningkat pada t 30 sedangkan mulai dari C 11 hingga C 17 secara berangsur-angsur kelimpahan relatifhya semakin menurun. Penurunan terbesar terjadi pada C 14 , yaitu semula 85,28% menjadi 43,11%. Di akhir penelitian berhasil diidentifikasi 7 jenis bakteri, dan 5 jenis di antaranya bergenus Bacillus yang bersifat aerobik.

The Influence of Microbial Consortium in Bioremediation Process using Bioreactor

“…Therefore, soil moisture is maintained in the range of 60-80 % due to soil microbes require sufficient moisture to grow [6]. Figure 5 shows a decrease in the pH of the soil due to lower soil moisture.…”

“…Below pH 5 and above 8.5, the bacteria cannot grow well unless the bacteria resistant to acid and alkaline [4]. On the other hand, the availability of oxygen is another environmental factor that is important in the biodegradation of oil [6]. Aeration is one way to provide oxygen for the microbes.…”

The potential of yeasts from the oil-contaminated soil and mangrove rhizosphere for degrading crude oil

“…Cookson (1995) stated that hydrocarbons are used as sources of nutrients and energy sources by microbes. Furthermore, Nugroho (2006) stated that the abundance and diversity of microorganisms in nature has a positive correlation with the percentage of oil degradation. Figure 4 showed that almost all treatments have optimum soil moisture content with an average of 25 -34%.…”

“…Measurements of soil moisture content were carried out to monitor soil water content in the reactor. Water content affects the onset of contamination, gas transfer, and the growth of microorganisms (Nugroho 2006). At the optimum moisture, microorganisms are active and grow faster.…”

Acreditation Certificate Acreditation No. 21/E/KPT/2018 Article Tools Print this article Indexing metadata How to cite item Email this article Email the author About The Authors Ainun Ramadhani Tri Wahyuni ORCID iD https://orcid.org/0000-0002-4071-3406 Fisheries and Marine Science Faculty, Brawijaya University Indonesia Endang Yuli Herawati Fisheries and Marine Science Faculty, Brawijaya University Indonesia Andi Kurniawan ORCID iD Fisheries and Marine Science Faculty, Brawijaya University Indonesia Ab

“…After the stationary phase is followed by a decrease because the number of bacteria is out of balance with the availability of nutrients. According to Nugroho (2012), hydrocarbon bacteria in their life activities require carbon molecules as a source of nutrition and energy to metabolize and reproduce. Another factor that affects the reduction in bacterial cell density is that there is a lack of nutrients such as N, P and C that are no longer suitable for growth.…”