Biodegradable Polymers Grafted onto Multifunctional Mesoporous Silica Nanoparticles for Gene Delivery

Abstract

Biodegradable polymer possesses significant potential for applications in different fields, since flexibility gives rise to materials with great physical and mechanical property diversity. The poly-caprolactone (PCL) and chitosan derivatives (CS) have the ability to form scaffolds, which adhere to the surface of mesoporous silica nanoparticles (MSNs) and its porous networks. The novel characteristics of the developed PCL/MSNs and CS/MSNs, such as very low in vivo degradation rate, ordered pore network, uniform and tunable size and shape of the particles, high pore volume and surface area, non-toxicity, and biocompatibility, among others, are responsible for its favorable gene delivery device and makes this conjugation a very good biomaterial for this application. In the present study, we investigated the synthesis of silica nanoparticles MCM-41 covalently grafted with PCL and CS and their use as a potential small interfering RNA (siRNA) carrier. The physical-chemical and morphological characterizations, as well as the applicability of functionalized MSNs as platforms for gene delivery, were assessed. Our results confirmed that MSNs that were successfully functionalized with PCL and CS kept their typical morphology and pore arrangement. Furthermore, their surface modification was successfully held. In vitro biocompatibility and cytotoxicity assays suggest the ability of MSNs to support passive uptake and indicated the potential of this material as a gene delivery system for cervical cancer cells (HeLa).

New Hydrogen-Bond-Enriched 1,3,5-Tris(2-hydroxyethyl) Isocyanurate Covalently Functionalized MCM-41: An Efficient and Recoverable Hybrid Catalyst for Convenient Synthesis of Acridinedione Derivatives

“…29,86,93,94 These results also indicate that 1,3,5-tris(2-hydroxyethyl)isocyanurate has been successfully grafted onto the surface of MCM-41. 95…”

<p>In vitro and in vivo Evaluation of Succinic Acid-Substituted Mesoporous Silica for Ammonia Adsorption: Potential Application in the Management of Hepatic Encephalopathy</p>

“…After performing motor co-ordination tests to determine the biochemistry of blood, animals were anesthetized with thiopental (80 mg/kg, i.p). Then, blood was collected from the abdominal aorta, moved to standard tubes (Heparin-coated tubes), and centrifuged (3000 g, for 15…”

“… 9 In past years, various investigations have been carried out on the adsorption of heavy metals by mesoporous silica. 10 , 11 These studies resulted in the development of a novel application for MS materials in sensors, 12 catalysts, 13 drug delivery carrier, 14 gene delivery, 15 an adsorbent 16 , 17 and also as an antidote agent. 13 In this regard, Farjadian et al reported that EDTA modified MS could be potent antidote agents while assessed in vitro and in vivo in adsorption of iron 18 and copper.…”

Localization of Coated Iron Oxide (Fe3O4) Nanoparticles on Tomato Seeds and Their Effects on Growth

“…Polycaprolactone (PCL) was bound on to the NH 2 -MNP particles via thermal coupling, based on a method published previously. 18 In brief, 100 mg of NH 2 -MNP (from section 2.2.2) were suspended in 20 mL of dry toluene in a round bottom flask (250 mL) and sonicated at 70°C until full dispersion. PCL (2 g) was dissolved separately in 20 mL of dry toluene and heated to 70°C.…”