Biocompatible Materials Enabled by Biobased Production of Pyomelanin Isoforms Using an Engineered Yarrowia lipolytica

Abstract

Melanin-like compounds have been studied in recent years for their electron transport and ultraviolet (UV) light absorbance properties as well as applications as functional, biocompatible catalysts, and material additives. Pyomelanin is a unique form of melanin compounds that has not received significant attention. Here, a strain of Yarrowia lipolytica suitable for the production of nearly 2.8 g L −1 of homogentisic acid (HGA) is metabolically engineered, which can then be oxidized to form pyomelanin either in situ or through altering pH. By using this biosourced material, a series of material traits including spectral analysis/UV-vis absorbance properties, electronic and metal interaction/chelation properties, and effectiveness in polymer dispersions with poly(l-lactide) are evaluated. In all cases, biosourced pyomelanin performs on par with or better than a chemically sourced analog. In a performance application, it is explored how pyomelanin may be blended at low concentrations to increase the elasticity of a rigid commercial polymer. Collectively, this work establishes biosourced pyomelanin as a versatile compound for unique material applications.

Bioproduction and optimization of newly characterized melanin pigment from Streptomyces djakartensis NSS-3 with its anticancer, antimicrobial, and radioprotective properties

“…8 B) showed a number of significant peaks that suggest the following peak assignments: OH group (3442.31 cm −1 ), CH 2 stretching (2372.01 cm −1 ), C = O or aromatic stretching (1645.95 cm −1 ), ionized COO − groups (1397.17 cm −1 ), combination of C-O bonds (1105.01 cm −1 ) and weak bands below 700 cm −1 represent the alkene C-H replacement in melanin. These FTIR features are identical with those of pyomelanin produced by different microorganisms [ 88 – 90 ]. Moreover, the molecular vibration and crystal structures of the purified melanin were analyzed using Raman spectroscopy.…”