Biocompatibility evaluation of silk fibroin with peripheral nerve tissues and cells in vitro

Yang, Yumin; Chen, Xuemei; Ding, Fei; Zhang, Peiyun; Liu, Jie; Gu, Xiaosong

doi:10.1016/j.biomaterials.2006.12.004

Cited by 306 publications

(202 citation statements)

References 48 publications

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“…Moreover, their rapid self-assembly enables cell encapsulation and irregular defect filling in both in vitro and in vivo applications. [11][12][13][14] In the present study, the histological and morphological observations at 4 weeks revealed significant changes in the number, diameter, and myelin sheath thickness of nerve fibers in the SF16 peptide hydrogel group after 4 weeks of implantation, indicating better nerve regeneration. These findings indicate that the SF16 peptide scaffold may have a positive effect in nerve regeneration, and therefore has potential as a matrix for nerve cell propagation.…”

Section: Histological Observationssupporting

confidence: 57%

“…11 Other results have also shown that dissociated dorsal root ganglion neurons can extend long neurites along silk fibers from Bombyx mori silkworms and Schwann cells are able to survive, adhere, and migrate along the silk fibers, suggesting that silk fibroin has potential as a scaffold material to support peripheral nerve regeneration. 12 Thus, peptide hydrogel scaffolds provide a platform that makes them ideal for nanomedical applications. Peptide hydrogel scaffolds not only have all of the advantages of traditional hydrogels but also do not use harmful materials to initiate the solution-gel transformation, therefore the degradation products are natural amino acids which can be metabolized.…”

Section: Introductionmentioning

confidence: 99%

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Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold

Wei¹,

Yao²,

Wang³

et al. 2013

IJN

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Background: Peripheral nerve injury is a common trauma, but presents a significant challenge to the clinic. Silk-based materials have recently become an important biomaterial for tissue engineering applications due to silk's biocompatibility and impressive mechanical and degradative properties. In the present study, a silk fibroin peptide (SF16) was designed and used as a component of the hydrogel scaffold for the repair of peripheral nerve injury. Methods: The SF16 peptide's structure was characterized using spectrophotometry and atomic force microscopy, and the SF16 hydrogel was analyzed using scanning electron microscopy. The effects of the SF16 hydrogel on the viability and growth of live cells was first assessed in vitro, on PC12 cells. The in vivo test model involved the repair of a nerve gap with tubular nerve guides, through which it was possible to identify if the SF16 hydrogel would have the potential to enhance nerve regeneration. In this model physiological saline was set as the negative control, and collagen as the positive control. Walking track analysis and electrophysiological methods were used to evaluate the functional recovery of the nerve at 4 and 8 weeks after surgery. Results: Analysis of the SF16 peptide's characteristics indicated that it consisted of a welldefined secondary structure and exhibited self-assembly. Results of scanning electron microscopy showed that the peptide based hydrogel may represent a porous scaffold that is viable for repair of peripheral nerve injury. Analysis of cell culture also supported that the hydrogel was an effective matrix to maintain the viability, morphology and proliferation of PC12 cells. Electrophysiology demonstrated that the use of the hydrogel scaffold (SF16 or collagen) resulted in a significant improvement in amplitude recovery in the in vivo model compared to physiological saline. Moreover, nerve cells in the SF16 hydrogel group displayed greater axon density, larger average axon diameter and thicker myelin compared to those of the group that received physiological saline. Conclusion: The SF16 hydrogel scaffold may promote excellent axonal regeneration and functional recovery after peripheral nerve injury, and the SF16 peptide may be a candidate for nerve tissue engineering applications.

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Section: Histological Observationssupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold

Wei¹,

Yao²,

Wang³

et al. 2013

IJN

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“…Their effects on dorsal root ganglia and Schwann cells show their biocompatibility and ability to promote cell growth. 67,68 Two types of fibers have been found, including spider silk fibers and silk worm fibers for tissue reconstruction in tissue engineering, particularly in nerve tissue engineering applications. In addition to adhesion, they are able to support cell migration and are biocompatible and biodegradable; however, their tedious extraction method is a major limitation.…”

Section: Silk Fibers To Enhance Nerve Regenerationmentioning

confidence: 99%

“…Yang et al showed that the silk fibroin and the fibroin fibers derived from the solution had good biologic compatibility with cells without cytotoxic effects. 67,68 Allmeling et al showed that spider silk fibers can be used as an innovative material in a biocompatible artificial nerve conduit. 69 …”

Section: Silk Fibers To Enhance Nerve Regenerationmentioning

confidence: 99%

Types of neural guides and using nanotechnology for peripheral nerve reconstruction

Biazar

Khorasani²,

Montazeri³

et al. 2010

IJN

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Peripheral nerve injuries can lead to lifetime loss of function and permanent disfigurement. Different methods, such as conventional allograft procedures and use of biologic tubes present problems when used for damaged peripheral nerve reconstruction. Designed scaffolds comprised of natural and synthetic materials are now widely used in the reconstruction of damaged tissues. Utilization of absorbable and nonabsorbable synthetic and natural polymers with unique characteristics can be an appropriate solution to repair damaged nerve tissues. Polymeric nanofibrous scaffolds with properties similar to neural structures can be more effective in the reconstruction process. Better cell adhesion and migration, more guiding of axons, and structural features, such as porosity, provide a clearer role for nanofibers in the restoration of neural tissues. In this paper, basic concepts of peripheral nerve injury, types of artificial and natural guides, and methods to improve the performance of tubes, such as orientation, nanotechnology applications for nerve reconstruction, fibers and nanofibers, electrospinning methods, and their application in peripheral nerve reconstruction are reviewed.

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“…SF is highly biocompatible and able to support appropriate cellular activity without eliciting rejection, inflammation or immune activation in the host [85]. Fibers of fibroin meshes have also been coated with PPy by chemical polymerization.…”

Section: Biodegradable Scaffolds Constituted By Nanofibers That Incormentioning

confidence: 99%

Nanomembranes and Nanofibers from Biodegradable Conducting Polymers

et al. 2013

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Abstract:This review provides a current status report of the field concerning preparation of fibrous mats based on biodegradable (e.g., aliphatic polyesters such as polylactide or polycaprolactone) and conducting polymers (e.g., polyaniline, polypirrole or polythiophenes). These materials have potential biomedical applications (e.g., tissue engineering or drug delivery systems) and can be combined to get free-standing nanomembranes and nanofibers that retain the better properties of their corresponding individual components. Systems based on biodegradable and conducting polymers constitute nowadays one of the most promising solutions to develop advanced materials enable to cover aspects like local stimulation of desired tissue, time controlled drug release and stimulation of either the proliferation or differentiation of various cell types. The first sections of the review are focused on a general overview of conducting and biodegradable polymers most usually employed and the explanation of the most suitable techniques for preparing nanofibers and nanomembranes (i.e., electrospinning and spin coating). Following sections are organized according to the base conducting polymer (e.g., Sections 4-6 describe hybrid systems having aniline, pyrrole and thiophene units, respectively). Each one of these sections includes specific subsections dealing with applications in a nanofiber or nanomembrane form. Finally, miscellaneous systems and concluding remarks are given in the two last sections. OPEN ACCESSPolymers 2013, 5 1116

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Biocompatibility evaluation of silk fibroin with peripheral nerve tissues and cells in vitro

Cited by 306 publications

References 48 publications

Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold

Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold

Types of neural guides and using nanotechnology for peripheral nerve reconstruction

Nanomembranes and Nanofibers from Biodegradable Conducting Polymers

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