BIOCHIP mosaic for the diagnosis of autoimmune bullous diseases in Chinese patients

Wang, Mingyue; Li, Furong; Xue, Wei; Wang, Rui; Chen, Tiancheng; Zhao, Jian; Chen, Xixue; Zhu, Xuejun

doi:10.1684/ejd.2020.3839

Cited by 3 publications

(2 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on the Biochip ® technology, in which multiple miniature substrates are placed in a single incubation field mounted to a standard laboratory slide, additional assays were developed, e.g., the Biochip ® mosaic containing monkey esophagus, primate salt-split skin, recombinant BP180 NC16A, and human HEK293 cells that recombinantly express the immunodominant regions of desmoglein 1, desmoglein 3, and BP230 on their surface (19). This Biochip ® mosaic is now widely used and has further been extended by cells that express type VII collagen and laminin 332 (20)(21)(22)(23)(24)(25)(26)(27)(28). Future studies will address the detection of serum IgG against further target antigens such as the p200 protein, the ectodomain of BP180, and IgA autoantibodies in AIBDs.…”

Section: Cooperation With Euroimmunmentioning

confidence: 99%

A life for autoimmune blistering diseases: in memoriam Detlef Zillikens

Hundt,

Sadik,

van Beek

et al. 2023

Front. Immunol.

View full text Add to dashboard Cite

Detlef Zillikens, MD, director and chair of the Department of Dermatology at the University of Lübeck, Lübeck, Germany, died in September 2022, aged only 64. He dedicated his professional life to autoimmune blistering diseases (AIBDs) and built his department into one of the world’s leading centers for these diseases. Herein, his professional life and the impact on the field of AIBDs and the research landscape at the University of Lübeck are addressed. With his warm, integrative, open-minded, ever-optimistic attitude, he was a highly reliable colleague, mentor, and friend to many in the field including each of the authors. Combined with his in-depth knowledge of dermatology, interest in many fields of life science, and hard work, Detlef Zillikens initiated the founding of two independent research institutes, the Lübeck Institute of Experimental Dermatology and the Institute and Comprehensive Center for Inflammation Medicine. He was also instrumental in establishing the Center for Research on Inflammation of the Skin, where in a new research building, over 140 scientists pursue research questions related to skin inflammation. By inviting numerous researchers and clinicians to his department and hosting two large international meetings, he brought the field of AIBDs much closer together and inspired multiple national and international research initiatives. His ideas will live on and grow in many of his colleagues and mentees.

show abstract

Section: Cooperation With Euroimmunmentioning

confidence: 99%

A life for autoimmune blistering diseases: in memoriam Detlef Zillikens

Hundt,

Sadik,

van Beek

et al. 2023

Front. Immunol.

View full text Add to dashboard Cite

show abstract

“…In addition to recombinant proteins such as BP180 NC16A and cells recombinantly expressing DSG1, DSG3, BP230, type VII collagen, and laminin 332, tissue substrates such as monkey esophagus and primate salt-split skin can also be used (6,(9)(10)(11). The BIOCHIP ® technology is increasingly used for routine diagnostics of autoimmune blistering diseases (AIBD) (12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22).…”

Section: Introductionmentioning

confidence: 99%

Computer-aided classification of indirect immunofluorescence patterns on esophagus and split skin for the detection of autoimmune dermatoses

et al. 2023

View full text Add to dashboard Cite

Autoimmune bullous dermatoses (AIBD) are rare diseases that affect human skin and mucous membranes. Clinically, they are characterized by blister formation and/or erosions. Depending on the structures involved and the depth of blister formation, they are grouped into pemphigus diseases, pemphigoid diseases, and dermatitis herpetiformis. Classification of AIBD into their sub-entities is crucial to guide treatment decisions. One of the most sensitive screening methods for initial differentiation of AIBD is the indirect immunofluorescence (IIF) microscopy on tissue sections of monkey esophagus and primate salt-split skin, which are used to detect disease-specific autoantibodies. Interpretation of IIF patterns requires a detailed examination of the image by trained professionals automating this process is a challenging task with these highly complex tissue substrates, but offers the great advantage of an objective result. Here, we present computer-aided classification of esophagus and salt-split skin IIF images. We show how deep networks can be adapted to the specifics and challenges of IIF image analysis by incorporating segmentation of relevant regions into the prediction process, and demonstrate their high accuracy. Using this semi-automatic extension can reduce the workload of professionals when reading tissue sections in IIF testing. Furthermore, these results on highly complex tissue sections show that further integration of semi-automated workflows into the daily workflow of diagnostic laboratories is promising.

show abstract