Biochemical responses of the marine mussel Mytilus galloprovincialis to petrochemical environmental contamination along the North-western coast of Portugal

Lima, Inês; Moreira, Susana; Osten, Jaime Rendón-von; Soares, Amadeu M.V.M.; Guilhermino, Lúcia

doi:10.1016/j.chemosphere.2006.07.057

Cited by 234 publications

(89 citation statements)

References 49 publications

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“…Evidence suggests that biochemical metabolites increased due to metal exposure in bivalve, the mussel Perna perna [15]. These results are comparable to the study, where antioxidant enzyme activity increases at sites contaminated with metals [16]. Perna indica exposed to cadmium, copper, lead and zinc concentrations under chronic toxicity showed significant differences in antioxidant enzyme activity [17].…”

Section: Resultssupporting

confidence: 78%

“…Perna indica exposed to cadmium, copper, lead and zinc concentrations under chronic toxicity showed significant differences in antioxidant enzyme activity [17]. Elevated reduced glutathione (GSH) activity was observed in mollusks exposed to petrochemical products [16]. Increased GST activity exposed to zinc and copper suggests that higher pollutant stress may have induced GST expression in order to protect tissues from xenobiotic damage [18].…”

Section: Resultsmentioning

confidence: 99%

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Reduced glutathione and acetylcholinesterase expressions in Perna indica exposed to trivalent arsenic

Rajkumar¹

2013

International Journal of Biological Research

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In the present study Indian brown mussel, Perna indica was exposed to 20, 40, 80, 160 and 320 ppb (µg/l) arsenic concentrations under short-term chronic toxicity bioassay (14 days) for the assessment of reduced glutathione and acetylcholinesterase as potential biochemical biomarkers in arsenic pollution. A higher level of reduced glutathione (GSH) and increased acetylcholinesterase (AchE) activity was expressed when compared to control in 160 and 320 ppb arsenic concentration. The found values were significant at P<0.01. The values of total protein were significantly lower (P<0.05) in all the exposed concentration when compared with control. This study suggests that metabolites and antioxidant enzymes activity was affected by heavy metal exposures and strongly have the potential as indicators of heavy metal contamination and arsenic in specific. Hence, the determination of oxidative stress biomarkers in Perna indica may serve as a convenient approach during pollution biomonitoring programme.

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Section: Resultssupporting

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Reduced glutathione and acetylcholinesterase expressions in Perna indica exposed to trivalent arsenic

Rajkumar¹

2013

International Journal of Biological Research

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“…This may be a reflection either of a decrease in contaminants inducing LPO or an adaptive antioxidant response over time to counteract ROS generation leading to a lower lipid peroxidation. M. galloprovincialis from low to high impacted sites on the NW coast of Portugal (Lima et al 2007) and Balearic Islands in Spain (Box et al 2007) showed slightly higher LPO values (9-12 and 7.7-12.1 μmol g -1 Prot, respectively) compared to the ones obtained in this study, which are indicative of the low or moderate levels of contamination found in mussels from the Ria Formosa Lagoon (Cravo et al 2009). As for clams, LPO values were similar to those found for the same species in the same lagoon in previous years (0.4-0.6 μmol g -1 Prot, Géret and Bebianno 2004;Bebianno and Barreira, 2009) and relatively lower than in Thau lagoon in France (0.643 μmol g -1 Prot, Bebianno et al 2004).…”

Section: Mytilus Galloprovincialiscontrasting

confidence: 40%

Interspecific variability of endocrine disruption and oxidative stress in two bivalve species from the Ria Formosa Lagoon (south coast of Portugal)

Pereira¹,

Gomes²,

Cardoso³

et al. 2013

Sci. Mar.

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SUMMARY: Estuaries are highly productive ecosystems that have been increasingly affected by anthropogenic activities. In this study, the presence of endocrine disrupting compounds (EDCs) and their relation to oxidative stress were assessed in two bivalve species (mussels Mytilus galloprovincialis and clams Ruditapes decussatus) from the Ria Formosa Lagoon (south Portugal). We applied the alkali-labile phosphate (ALP) assay to determine vitellogenin-like protein levels, and the lipid peroxidation (LPO) method to assess oxidative damage. Mussels and clams exhibited the same pattern of ALP concentrations, with no differences between genders and higher levels in winter. R. decussatus showed higher ALP values than M. galloprovincialis, possibly associated with a stronger responsiveness of clams to EDCs or due to the influence of the reproductive cycle. Mussels exhibited a more evident seasonal effect for LPO, indicating that these organisms are more influenced by seasonal fluctuations in the water column compared to the sediment-associated clams. Both species appear to be under the influence of EDC exposure (which could induce Vg-like protein synthesis) and oxidative stressors (leading to lipid peroxidation) in the Ria Formosa Lagoon, particularly in winter, reflecting the presence of different contaminants in Ria Formosa and environmental changes, as well as complex interactions between them.Keywords: Ria Formosa Lagoon, Mytilus galloprovincialis, Ruditapes decussatus, endocrine disruption, vitellogenin-like proteins, lipid peroxidation. RESUMEN: Variabilidad interespecífica de disruptores endocrinos y estrés oxidatiVo en dos especies de biValVos de la ría formosa (costa sur de portugal). -Los estuarios son ecosistemas altamente productivos afectados por actividades antropogénicas. En este trabajo, la presencia de compuestos disruptores endocrinos (EDCs) y su relación con estrés oxidativo se ha evaluado en los bivalvos Mytilus galloprovincialis y Ruditapes decussatus en la laguna Ría Formosa (Portugal). Se utilizó el ensayo de fosfatos lábil alcalinos (ALP) para determinar los niveles de proteína tipo-vitelogenina y el método de peroxidación lipídica (LPO) para evaluar los daños oxidativos. Ambas especies exhibieron el mismo patrón de concentración de ALP: no hubo diferencias entre sexos pero si niveles más altos en invierno. R. decussatus presentó valores mayores de ALP que M. galloprovincialis posiblemente asociados a una capacidad de respuesta más elevada a los EDCs o al estado del ciclo reproductor. Los mejillones, al no estar asociados al sedimento como las almejas, mostraron variaciones estacionales en las concentraciones de LPO, indicando mayor sensibilidad a fluctuaciones ambientales en la columna de agua. Ambas especies parecen estar influenciadas (principalmente en invierno) por EDCs (posiblemente induciendo la síntesis de proteína tipo-vitelogenina) y factores de estrés oxidativo (activando la peroxidación lipídica); lo que refleja la presencia de diferentes contaminantes en la Ría Formosa y los cambios a...

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“…In this case, both genes were up-regulated (Table 3) with the A form showing a general up-regulation in response to all three chemicals, but with the B form, more specific to PHE. Catalytic activities of SOD and CAT have been identified as being involved in the cellular protection against ROS of molluscs (mussels and limpets) challenged with environmental pollutants and environmental stress (Ansaldo et al, 2005;Cheung et al, 2004;Lima et al, 2007;Zhang et al, 2011). However, some discrepancy between gene transcription and enzymatic activity of both antioxidants has recently been reported in Mytilus edulis challenged with chemical stressors (Giuliani et al, 2013).…”

Section: Detection Of Antioxidant Genes Of Interest For Ecotoxicologymentioning

confidence: 99%

Key metabolic pathways involved in xenobiotic biotransformation and stress responses revealed by transcriptomics of the mangrove oyster Crassostrea brasiliana

et al. 2015

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Key metabolic pathways involved in xenobiotic biotransformation and stress responses revealed by transcriptomics of the mangrove oyster Crassostrea brasiliana.Aquatic Toxicology http://dx.doi.org/10. 1016/j.aquatox.2015.06.012 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Highlights-Thetranscriptome of the mangrove oyster Crassostrea brasilianawas sequenced.-The sequencing effort dramatically expanded the existing cDNA sequences available for the species.-Global analysis for transcription in the oyster treated with phenanthrene, diesel and domestic sewage was performed.-The pollutants altered mRNAs for genes in biotransformation, antioxidant and stress response pathways. Abstract 2The Brazilian oyster Crassostrea brasiliana was challenged to three common environmental contaminants: phenanthrene, diesel fuel water-accommodated fraction (WAF) and domestic sewage. Total RNA was extracted from the gill and digestive gland, and cDNA libraries were sequenced using the 454 FLX pla orm. The assembled transcriptome resulted in ˜20,000contigs, which were annotated to produce the first de novo transcriptome for C. brasiliana.Sequences were screened to identify genes potentially involved in the biotransformation of xenobiotics and associated antioxidant defence mechanisms. These gene families included those of the cytochrome P450 (CYP450), 70kDa heat shock, antioxidants, such as glutathione Stransferase, superoxide dismutase, catalase and also multi-drug resistance proteins. Analysis showed that the massive expansion of the CYP450 and HSP70 family due to gene duplication identified in the Crassostrea gigas genome also occurred in C. brasiliana, suggesting these processes form the base of the Crassostrea lineage. Preliminary expression analyses revealed several candidates biomarker genes that were up-regulated during each of the three treatments, suggesting the potential for environmental monitoring.

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Biochemical responses of the marine mussel Mytilus galloprovincialis to petrochemical environmental contamination along the North-western coast of Portugal

Cited by 234 publications

References 49 publications

Reduced glutathione and acetylcholinesterase expressions in Perna indica exposed to trivalent arsenic

Reduced glutathione and acetylcholinesterase expressions in Perna indica exposed to trivalent arsenic

Interspecific variability of endocrine disruption and oxidative stress in two bivalve species from the Ria Formosa Lagoon (south coast of Portugal)

Key metabolic pathways involved in xenobiotic biotransformation and stress responses revealed by transcriptomics of the mangrove oyster Crassostrea brasiliana

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