Biochemical differences among human and animal streptococci of Lancefield group C or group G

Efstratiou, Androulla; Colman, G.; Hahn, Gabriele; Timoney, John F.; Boeufgras, J. M.; Monget, D.

doi:10.1099/00222615-41-2-145

Cited by 42 publications

(37 citation statements)

References 8 publications

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“…There was one prevalent biotype among the isolated strains which produced phosphatase, leucine amidopeptidase, arginine dihydrolase, alpha-D-and beta-D-galactosidase and fermented lactose and ribose. Results from the biochemical tests that are typically variable (including pyrrolidonylarylamidase, alpha-D-and beta-D-galactosidase, beta-D-glucuronidase, and acidification of lactose and trehalose) were generally within the range of proportions reported for S. canis by other researchers (Clark et al 1984;Devriese et al 1986;Efstratiou et al 1994;Vieira and Castro 1994;Soedarmanto and Lammler 1996). Only 16.3% of S. canis strains were positive for esculin hydrolysis, which is in contrast to the results of Devriese et al (1986) and Vieira and Castro (1994), who found 100% and 90% of strains positive, respectively.…”

Section: Resultsmentioning

confidence: 53%

“…Only 16.3% of S. canis strains were positive for esculin hydrolysis, which is in contrast to the results of Devriese et al (1986) and Vieira and Castro (1994), who found 100% and 90% of strains positive, respectively. On the other hand, Efstratiou et al (1994) described only 40% of strains as esculin positive and all positive reactions were reported as delayed, which might be responsible for some of the discrepancy. According to Hassan et al (2005) all S. canis isolates appeared to be esculin negative on primary culture; whereas, the isolates were uniformly positive after they were subcultured.…”

Section: Resultsmentioning

confidence: 84%

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Prevalence and Characteristics of Streptococcus canis Strains Isolated from Dogs and Cats

et al. 2007

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To determine the prevalence of Streptococcus canis in dogs and cats, a total of 926 swabs were examined bacteriologically in the period from 2003 to 2005. Eighty-six isolates obtained from various anatomical locations were further characterized for their phenotypic properties. The most frequently isolated biotype produced phosphatase, leucine amidopeptidase, arginine dihydrolase, alpha-D-and beta-D-galactosidase and fermented lactose and ribose. Additional identification by species-specific amplification of the 16S-23S rRNA intergenic spacer region was consistent with S. canis. All isolates were susceptible to penicillin G and ampicillin. The least effective antimicrobial agent was found to be tetracycline (only 33.8% of susceptible strains). Prevalence, S. canis, biochemical properties, 16S-23S rRNA intergenic spacer region

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Section: Resultsmentioning

confidence: 53%

Section: Resultsmentioning

confidence: 84%

Prevalence and Characteristics of Streptococcus canis Strains Isolated from Dogs and Cats

et al. 2007

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“…HhaI, MboII, and Sau3A patterns are quite similar for these three species as well, although DNA homology between species is between 45 and 75% (32) and 16S rRNA similitude value is limited to 96 to 97% (1). However, the classification of this group remained controversial as no test was clearly capable of differentiating these species (9,29,32).…”

Section: Discussionmentioning

confidence: 99%

“…Identification of streptococcal species is currently based on observation of the cultural and morphological characteristics, determination of the biochemical pattern (production of enzymes and production of acid from various carbohydrate sources) (4,5,6,9,11,17,20), and observation of the antigenic structure according to the classification of Rebecca Lancefield (22).…”

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confidence: 99%

Identification of Major Streptococcal Species by rrn -Amplified Ribosomal DNA Restriction Analysis

Schlegel

Grimont

et al. 2003

J Clin Microbiol

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Amplified ribosomal DNA restriction analysis (rrn-ARDRA) is based on PCR amplification and restriction of a fragment of rRNA genes including 16S and 23S genes and the intergenic spacer. rrn-ARDRA was evaluated for the identification of species within the genus Streptococcus. A total of 148 type and reference strains of pyogenic, oral, and group D streptococci were examined in order to construct a database for identification of streptococci. The amplified product was a single band approximately 4,500 bp long. This amplicon was digested separately with three (HhaI, MboII, and Sau3A) restriction endonucleases. Respectively, 27, 26, and 28 major patterns were observed after HhaI, MboII, and Sau3A restrictions. Streptococcal strains belonging to different species had different patterns or different combination of patterns. An identification system based upon a combination of the three restriction patterns in a single database was then proposed. rrn-ARDRA was successfully applied to 11 clinical isolates whose identification to the species level was difficult to obtain by phenotypic analysis. Using a database of well-characterized strains, rrn-ARDRA is a powerful method for the identification of streptococcal isolates.Streptococci are major pathogens for animals and human beings. Phenotypic studies and DNA-DNA hybridization assays have demonstrated that the genus Streptococcus may be divided in three major groups of species: pyogenic, oral, and group D streptococci (5,6,17,20,21,27).Identification of streptococcal species is currently based on observation of the cultural and morphological characteristics, determination of the biochemical pattern (production of enzymes and production of acid from various carbohydrate sources) (4,5,6,9,11,17,20), and observation of the antigenic structure according to the classification of Rebecca Lancefield (22).Molecular tools as ribotyping (3,8,25,26), oligonucleotide probing (2), PCR-based protocols (13, 23), and DNA sequence analysis (1,19,24) are now proposed to provide an accurate identification of streptococcal isolates. Among these, restriction fragment length polymorphism analysis was proposed as a general method for bacterial identification and typing (15). Amplified ribosomal DNA (rDNA) restriction analysis (ARDRA) was recently reported to be a rapid and efficient method of identification of bacterial isolates to the species level in mollicutes (7) and in some genera such as Acinetobacter or Mycobacterium (30, 31). The initial protocol was substantially improved by amplification of a larger fragment of the rrn operon (16S rRNA gene, 16S-23S intergenic spacer, and 23S rRNA gene) followed by the digestion with one to three endonucleases selected according to the bacterial genus studied (12, 18). The latest modification of this method uses a unique combination of three restriction endonucleases (HhaI, MboII, and Sau3A) irrespective of the bacterial species studied. This modified protocol was successfully used for identification of the genus and the species of strains belonging t...

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“…A number of biochemical characteristics are known to be useful in differentiating human GGS (S. dysgalactiae subsp. equisimilis) and animal GGS (S. canis), as illustrated in Table 1 (2,3,5,6). The most informative dis-criminatory tests are recognized to be the production of ␣-galactosidase and ␤-galactosidase and the production of acid from trehalose.…”

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confidence: 99%

Identification of Isolates ofStreptococcus canisInfecting Humans

et al. 2001

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During a survey of Group G and C streptococcal infections of humans two epidemiologically unrelated Group G streptococcal isolates were identified, one from a case of bacteremia and one from a wound infection. These isolates were atypical among this sample in that the emm gene could not be amplified from them by PCR. Biochemical characterization identified the isolates as Streptococcus canis, an organism normally associated with animal hosts. The biochemical identification was confirmed by sequencing of the 16S rRNA gene from both isolates and comparison with sequences of the S. canis type strain and other related streptococci of animals and humans. Comparative sequencing of fragments of two other housekeeping genes, sodA and mutS, confirmed that the isolates are most closely related to S. canis. The identification of two isolates of S. canis from a relatively small sample set suggests that the practice of identifying streptococci only by the Lancefield serological group may result in underestimation of the presence of S. canis in the human population.

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Biochemical differences among human and animal streptococci of Lancefield group C or group G

Cited by 42 publications

References 8 publications

Prevalence and Characteristics of Streptococcus canis Strains Isolated from Dogs and Cats

Prevalence and Characteristics of Streptococcus canis Strains Isolated from Dogs and Cats

Identification of Major Streptococcal Species by rrn -Amplified Ribosomal DNA Restriction Analysis

Identification of Isolates ofStreptococcus canisInfecting Humans

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