Biochemical characterization of pulmonary washings of patients with alveolar proteinosis, interstitial pneumonitis and alveolar cell carcinoma.

Onodera, Tsugutami; Nakamura, Mitsushige; Sato, Tadahiro; Akino, Toyoaki

doi:10.1620/tjem.139.245

Cited by 26 publications

(20 citation statements)

References 42 publications

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“…In our PAP cohort, we observed an exponential decay of the protein concentration during the lavage, similar to data published before [21,23,24,30]: the median initial protein concentration was at least 20-fold higher than the final concentration. We did not find a correlation with age, BMI or side lavaged.…”

Section: Discussionsupporting

confidence: 89%

“…The recovered fluid was centrifuged at 1,7206g for 10 min in order to separate water-insoluble particulate materials including cells and debris, as described by ONODERA et al [24]. In the supernatant, the total protein concentration was measured with a spectrophotometer in standardised volumes (10 mL) and in duplicate (Konelab T series for U/CSF protein; Thermo Fisher Scientific, Vantaa, Finland).…”

Section: Protein Concentrationmentioning

confidence: 99%

“…This technique has been improved over the years in order to enhance the removal of material from the alveoli [19][20][21][22][23]. Few studies have reported on the protein concentration, the kinetics of the wash-out process and the factors that can influence the biochemical composition of the fluid [15,24,25]. PEREZ and ROGERS [23] quantified and compared the effective alveolar clearance for each component of the lavage by measuring the dry weight of material in the lavage effluent in five patients.…”

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confidence: 99%

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Wash-out kinetics and efficacy of a modified lavage technique for alveolar proteinosis

Bonella

Bauer

Griese³

et al. 2012

Eur Respir J

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Whole lung lavage (WLL) is the standard treatment for pulmonary alveolar proteinosis (PAP). This study aimed to provide data about the kinetics of the protein wash-out, to identify factors influencing the protein concentration in the recovered fluid and to assess the efficacy of a modified lavage technique.Samples of 180 WLLs from 42 adult PAP patients were collected. 110 WLLs were performed according to the classical technique. In 70 WLLs, repeated manual ventilation was applied during the procedure. Spectrophotometry was used to measure the protein concentration in the recovered fluid.The initial protein concentration in the recovered fluid was 460 mg?dL -1 , the final concentration was 26 mg?dL -1 and the total amount of removed proteins during a lavage was 17.5 g. A history of dust exposure was associated with a higher residual protein concentration in the recovered fluid (p50.000013). The amount of removed proteins correlated inversely with the diffusing capacity of the lung for carbon monoxide (p50.001) and oxygen tension (p50.004). The modified technique removed a greater amount of proteins than the classical technique and prolonged the time to relapse (p50.011). Exposure to dust seems to influence the kinetics of the protein wash-out. Applying manual ventilation during the procedure can enhance the efficacy of WLL.

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Section: Discussionsupporting

confidence: 89%

Section: Protein Concentrationmentioning

confidence: 99%

mentioning

confidence: 99%

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Wash-out kinetics and efficacy of a modified lavage technique for alveolar proteinosis

Bonella

Bauer

Griese³

et al. 2012

Eur Respir J

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“…In an early outstanding paper, the lipid composition and in vivo synthesis of lipids in adult patients with PAP was described [57]. Similarly, AKINO and coworkers [41, 153,154] have collected detailed information on the biochemical nature of the surfactant lipids [155] and surfactant proteins from PAP patients. Two oligomeric forms, alveolar proteinosis protein (APP)-I, consisting of large SP-A multimers of 70±90 mm in size, and APP-II, hexameric SP-A particles, were isolated and investigated regarding their effects on isolated type II epithelial cells [156,157].…”

Section: Pulmonary Alveolar Proteinosismentioning

confidence: 99%

Increased expression of apoptosis signalling receptors by alveolar macrophages in sarcoidosis

Dai¹,

Guzman²,

Costabel³

1999

Eur Respir J

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The Fas receptor (FasR) and the tumour necrosis factor (TNF) receptor 55/60 kDa (TNFR1) are recognized as apoptosis signalling receptors. They are known to be expressed by lymphocytes in association with immune regulation and immunological disorders. This study aimed to investigate the expression of FasR and TNFR1 by alveolar macrophages (AM) in patients with sarcoidosis. Bronchoalveolar lavage (BAL) was performed in 12 patients with active sarcoidosis and 11 control subjects. BAL cells were characterized by monoclonal antibodies using a peroxidase-antiperoxidase method. Both FasR and TNFR1 were expressed on a higher percentage of AM in sarcoidosis with respect to control subjects (mean +/-sem 40.8+/-3.1% versus 14.9+/-1.7%, p<0.001 and 61.9+/-3.3% versus 23.1+/-4.1%, p<0.001, respectively). There was a close relationship between the expression of FasR and TNFR1 on AM (r = 0.86, p<0.001). The percentages of FasR+ AM and TNFR1+ AM were in direct proportion to the percentage of BAL lymphocytes (r = 0.75 and 0.84), the CD4/CD8 ratio (r = 0.78 and 0.78), and the percentage of the CD14+ AM subset (r = 0.77 and 0.87), p<0.001 for all correlations. This study indicates that alveolar macrophages expressing apoptotic receptors are increased in patients with active sarcoidosis. Further studies are required to determine whether these alveolar macrophages from patients with sarcoidosis undergo apoptosis more readily than those from control subjects.

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“…This material is composed of proteins and lipids and is thought to be derived from alveolar surfactant (2,3). It has been reported that carcinoembryonic antigen (CEA)is elevated in serum and in bronchoalveolar lavage fluid (BALF) (4)(5)(6)(7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

Change in Cytokeratin 19 Fragment Level According to the Severity of Pulmonary Alveolar Proteinosis.

et al. 2001

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A 48-year-old man was diagnosed with primary alveolar proteinosis (PAP). Whole lung lavage was performed for treatment, and the opacity on his chest X-ray completely disappeared. The value of cytokeratin 19 fragment (CYFRA) in the serum was high at the beginning, decreased after lung lavage, and became elevated again when the disease recurred 7 months later. As PAPis thought to be a problem of surfactant secreted from type II pneumocytes, and a cytokeratin is present in the alveolar epithelial tissue, the value of serum CYFRAmight be related to the severity of PAP.

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Biochemical characterization of pulmonary washings of patients with alveolar proteinosis, interstitial pneumonitis and alveolar cell carcinoma.

Cited by 26 publications

References 42 publications

Wash-out kinetics and efficacy of a modified lavage technique for alveolar proteinosis

Wash-out kinetics and efficacy of a modified lavage technique for alveolar proteinosis

Increased expression of apoptosis signalling receptors by alveolar macrophages in sarcoidosis

Change in Cytokeratin 19 Fragment Level According to the Severity of Pulmonary Alveolar Proteinosis.

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