Biochemical and molecular responses during overwintering of red clover populations recurrently selected for improved freezing tolerance

Bertrand, Annick; Rocher, Solen; Claessens, Annie; Bipfubusa, Marie; Papadopoulos, Y. A.; Castonguay, Yves

doi:10.1016/j.plantsci.2019.110388

Cited by 12 publications

(23 citation statements)

References 66 publications

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“…Moreover, our results showed that cold-acclimated plants of A-TF7 had a 17% larger biomass regrowth than the initial cultivar A-TF0 following a nonlethal freezing stress at -11°C. These results are consistent with previous work on recurrent selection to improve the freezing tolerance of alfalfa cultivar Apica (Castonguay et al 2009;Castonguay et al 2011) and thus, con rmed the effectiveness of this recurrent selection method performed indoor and under control conditions to improve the freezing tolerance in alfalfa and other perennial species (Bertrand et al 2020b).…”

Section: Discussionsupporting

confidence: 92%

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Impact of Sinorhizobium meliloti strains and plant population on regrowth and nodule regeneration of alfalfa after a freezing event

D’Amours¹,

Bertrand²,

Cloutier³

et al. 2022

Preprint

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Purpose The increase in frequency of freeze-thaw episodes with the diminution of snow cover protection due to climate change compromises the winter survival of alfalfa (Medicago sativa L.). Symbiosis with cold-tolerant rhizobial strains can improve the ability of alfalfa to survive and grow under stressful conditions. Methods Six strains of Sinorhizobium (Ensifer) meliloti were tested in combination with two alfalfa populations bred to differ in their levels of freezing tolerance. Plants of each different combination were grown for eight weeks in a growth chamber before being exposed to temperatures promoting their acclimation to cold. Plants were then exposed to a freezing stress (-11ºC) and regrown for three weeks. Shoot, root and nodule biomass were measured before cold acclimation and three weeks after the freezing stress. Results After freezing stress, the alfalfa population A-TF7 had shoot and root biomasses that were respectively 19% and 15% larger than cultivar A-TF0. Alfalfa plants inoculated with strain NRG34 showed both a larger shoot biomass and a higher nodule dry weight than plants inoculated with any other strains. Assessment of freezing damages on nodules showed that plants inoculated with NRG34 had the largest proportion of undamaged nodules or of nodules with a regeneration zone. Conclusion This study shows for the first time a relationship between nodule and shoot regrowth after a freezing stress, the latter being linked with the proportion of nodules showing less freezing damage. Our results demonstrated that both the choice of alfalfa populations and S. meliloti strains adapted to stress are complementary to increasing alfalfa persistence.

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Section: Discussionsupporting

confidence: 92%

“…The temperatures tested were -2, -14, -16, -18, -20, -22, -24, -26, and -28°C. After 3 weeks of regrowth, the survival count of the plants at each temperature were recorded and the LT 50 was calculated (Bertrand et al 2020b).…”

Section: Characterization Of Plant Materialsmentioning

confidence: 99%

Impact of Sinorhizobium meliloti strains and plant population on regrowth and nodule regeneration of alfalfa after a freezing event

D’Amours¹,

Bertrand²,

Cloutier³

et al. 2022

Preprint

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“…In order to measure the effect of one cycle of selection for reduced dormancy on freezing tolerance, the precise level of freezing tolerance was evaluated under simulated winter conditions in an unheated greenhouse as described in Bertrand, Rocher et al. (2020). Briefly, potted alfalfa plants of the four populations previously described (initial cultivars Peace and Yellowhead and PRD1 and YRD1) were transferred into the greenhouse to induce fall cold acclimation and overwintering conditions similar to field conditions.…”

Section: Methodsmentioning

confidence: 99%

Agronomical evaluation of low dormancy alfalfa populations selected by an indoor screening method

et al. 2022

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Fall dormancy is a vital component of alfalfa (Medicago sativa L.) yield in northern climates, but selection for the trait is often done at the expense of winter survival. We performed one cycle of selection to reduce fall dormancy in two winter hardy cultivars (Yellowhead and Peace) using a new indoor screening method. We compared the reduced dormancy populations with their respective initial cultivars for fall dormancy, yield, and winter survival at four sites across Canada. During the establishment and the first production years, plants of the reduced dormancy populations were generally taller in the fall than their respective cultivar, which resulted in a one unit increase of their fall dormancy class. Under field conditions, plants of the reduced dormancy populations had a similar winter survival than those of the initial cultivars. Under simulated winter conditions, freezing tolerance was not affected by selection for reduced dormancy in Peace, whereas a decrease from −24.0 to −21.5 ˚C was observed in Yellowhead. However, in this cultivar, we noted a 37% yield increase under field conditions and a 40% more vigorous regrowth under simulated winter conditions in the reduced dormancy population. These results showed that the indoor selection method effectively reduced fall dormancy and that indirect responses for yield and winter survival were dependent on the genetic background used as selection material. This selection method could therefore be promising to develop alfalfa cultivars adapted to northern latitudes with high winter hardiness and improved late season yield.

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“…The quality of the feed mass, plant disease resistance, seed yield, winter hardiness and competitiveness of each sample in a mixture with cereal and grass components are taken into account. The main marker feature in breeding will take into account the length of the corolla tube as a marker feature for pollination activity and seed formation [8][9].…”

Section: Methodsmentioning

confidence: 99%

Study of varieties of meadow clover by economically valuable characteristics

Kelekhsashvili

Lekova

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Biochemical and molecular responses during overwintering of red clover populations recurrently selected for improved freezing tolerance

Cited by 12 publications

References 66 publications

Impact of Sinorhizobium meliloti strains and plant population on regrowth and nodule regeneration of alfalfa after a freezing event

Impact of Sinorhizobium meliloti strains and plant population on regrowth and nodule regeneration of alfalfa after a freezing event

Agronomical evaluation of low dormancy alfalfa populations selected by an indoor screening method

Study of varieties of meadow clover by economically valuable characteristics

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