Biochemical analysis of enhanced tolerance in transgenic potato plants overexpressing d-galacturonic acid reductase gene in response to various abiotic stresses

Hemavathi,; Upadhyaya, Chandrama Prakash; Nookaraju, Akula; Kim, Hyun Soon; Jeon, Jae Heung; Ho, Oh Man; Chun, Se Chul; Kim, Doo Hwan; Park, Se Won

doi:10.1007/s11032-010-9465-6

Cited by 64 publications

(27 citation statements)

References 64 publications

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“…5B). Similar results were obtained in transgenic potato plants expressing ascorbate biosynthesisrelated genes, including strawberry D-galacturonic acid reductase gene and rat L-gulono-γ-lactone oxidase gene which resulted in improved survival under various abiotic stresses (Hemavathi et al 2009(Hemavathi et al , 2010(Hemavathi et al , 2011, especially with exposure to high salt concentrations. The MDA content in the wild-type and transformed plants was determined.…”

Section: Molecular Analysis Of T 1 Plantssupporting

confidence: 72%

“…Although levels of ascorbic acid are modest compared with some other vegetables, tomato ranks high as a source of vitamin C in human diet because of high consumption in many countries (Hanson et al 2004). AsA also acts as a cell signaling modulator in a wide array of crucial physiological processes in plants including cell division, growth regulation, and senescence (Smirnoff 1996;Pavet et al 2005;Hemavathi et al 2009Hemavathi et al , 2010Hemavathi et al , 2011. Moreover, ascorbate acts as a cofactor for many enzymes, thereby affecting the expression of genes involved in defense and hormone signaling pathways (Pastori et al 2003;Tullio and Arrigoni 2004).…”

Section: Introductionmentioning

confidence: 99%

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Over-expression of l-gulono-γ-lactone oxidase (GLOase) gene leads to ascorbate accumulation with enhanced abiotic stress tolerance in tomato

Lim

Pulla

Park

et al. 2012

In Vitro Cell.Dev.Biol.-Plant

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Vitamin C (ascorbic acid, AsA) is an essential component for collagen biosynthesis and also for correct functioning of the cardiovascular system in humans. Plants and several animals can synthesize ascorbic acid, whereas humans lack the gene essential for ascorbic acid biosynthesis and must acquire the vitamin from their diet. In the present study, we developed a transgenic tomato plant (Lycopersicon esculentum Mill.) over-expressing the rat L-gulono-γ-lactone oxidase (GLOase) gene driven by CaMV35S constitutive promoter. The overexpression of GLOase resulted in a 1.5-fold increase in AsA levels in transgenic tomato fruits, which was positively correlated with increased GLOase activity. The Southern and reverse transcriptase polymerase chain reaction results revealed the stable integration of the transgene in the progeny of the engineered plants. The transgenic plants with increased vitamin C content in the fruit showed enhanced tolerance to abiotic stresses induced by methyl viologen, NaCl, and mannitol as compared with wild-type plants. The leaf disc senescence assay showed better tolerance in transgenic plants by retaining higher chlorophyll content compared with wild-type plants. The T 1 plants survived under salt stress (200 mM NaCl) whereas wild-type plants were unable to survive such high salt concentrations. This study suggests that the increased accumulation of AsA could upregulate the antioxidant system which imparts improved tolerance against various abiotic stresses in transgenic tomato plants as compared with wild-type plants.

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Section: Molecular Analysis Of T 1 Plantssupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Over-expression of l-gulono-γ-lactone oxidase (GLOase) gene leads to ascorbate accumulation with enhanced abiotic stress tolerance in tomato

Lim

Pulla

Park

et al. 2012

In Vitro Cell.Dev.Biol.-Plant

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“…Nevertheless, it has also a fundamental importance in plants acting as an antioxidant against a wide variety of biotic and abiotic stresses (Gallie 2013), as a modulator in cell signaling (Hemavathi et al 2011) and as a cofactor in different processes (Chen and Gallie 2004;Kotchoni et al 2009). Tomato is one of the most important vegetable sources of ascorbic acid (AsA) in many countries (Hanson et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Integrated bioinformatics to decipher the ascorbic acid metabolic network in tomato

et al. 2016

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Ascorbic acid is involved in a plethora of reactions in both plant and animal metabolism. It plays an essential role neutralizing free radicals and acting as enzyme co-factor in several reaction. Since humans are ascorbate auxotrophs, enhancing the nutritional quality of a widely consumed vegetable like tomato is a desirable goal. Although the main reactions of the ascorbate biosynthesis, recycling and translocation pathways have been characterized, the assignment of tomato genes to each enzymatic step of the entire network has never been reported to date. By integrating bioinformatics approaches, omics resources and transcriptome collections today available for tomato, this study provides an overview on the architecture of the ascorbate pathway. In particular, 237 tomato loci were associated with the different enzymatic steps of the network, establishing the first comprehensive reference collection of candidate genes based on the recently released tomato gene annotation. The co-expression analyses performed by using RNA-Seq data supported the functional investigation of main expression patterns for the candidate genes and highlighted a coordinated spatial-temporal regulation of genes of the different pathways across tissues and developmental stages. Taken together these results provide evidence of a complex interplaying mechanism and highlight the pivotal role of functional related genes. The definition of genes contributing to alternative pathways and their expression profiles corroborates previous hypothesis on mechanisms of accumulation of ascorbate in the later stages of fruit ripening. Results and evidences here provided may facilitate the development of novel strategies for biofortification of tomato fruit with Vitamin C and offer an example framework for similar studies concerning other metabolic pathways and species.

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“…4a1). KSB may somewhat improve antioxidant enzyme activities via the enhancement of K + levels in maize plants, which is involved in the maintenance of the turgor pressure and mitigation of oxidative stress (Hemavathi et al 2011). Moreover, increased K + accumulation in maize plants may be related to the impact of KSB inoculation via membrane stability, facilitating compartmentalization within vacuoles and selective ion uptake, further improving antioxidant enzyme levels (Mantelin and Touraine 2004).…”

Section: Discussionmentioning

confidence: 99%

Effects of potassium‐solubulizing and photosynthetic bacteria on tolerance to salt stress in maize

et al. 2019

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Aims The purpose of this study was to determine the positive effects of potassium‐solubilizing bacteria and photosynthetic bacteria on the salt tolerance of maize. Methods and Results We selected the maize inbred lines USTB‐265 (salt‐sensitive), USTB‐109 (moderately salt‐tolerant) and USTB‐297 (salt‐tolerant) to investigate their growth characteristics, enzyme activity and gene expression in response to inoculation with photosynthetic bacteria and potassium‐solubilizing bacteria under salt‐stress conditions. Conclusions Photosynthetic bacteria and potassium‐solubilizing bacteria inoculation significantly enhanced the expression of antioxidant enzyme‐related genes and increased the activities of the antioxidant enzymes superoxide dismutase, catalase and ascorbate peroxidase. In addition, inoculation with photosynthetic bacteria more efficiently improved maize salt tolerance than inoculation with potassium‐solubilizing bacteria. While the effects of these bacteria differed among the three maize lines, both photosynthetic bacteria and potassium‐solubilizing bacteria can enhance salt tolerance in maize. Significance and Impact of the Study Soil salinization is one of the most critical factors affecting maize growth. These two types of bacteria (e.g. Bacillus mojavensis JK07 and Rhodopseudomonas palustris) have proven useful in salinized agricultural lands as bio‐inoculants to increase crop productivity.

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Biochemical analysis of enhanced tolerance in transgenic potato plants overexpressing d-galacturonic acid reductase gene in response to various abiotic stresses

Cited by 64 publications

References 64 publications

Over-expression of l-gulono-γ-lactone oxidase (GLOase) gene leads to ascorbate accumulation with enhanced abiotic stress tolerance in tomato

Over-expression of l-gulono-γ-lactone oxidase (GLOase) gene leads to ascorbate accumulation with enhanced abiotic stress tolerance in tomato

Integrated bioinformatics to decipher the ascorbic acid metabolic network in tomato

Effects of potassium‐solubulizing and photosynthetic bacteria on tolerance to salt stress in maize

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