Bioaugmentation with a consortium of bacterial nitrophenol-degraders for remediation of soil contaminated with three nitrophenol isomers

Chi, Xiang-Qun; Zhang, Jun‐Jie; Zhao, Shuo; Zhou, Ning‐Yi

doi:10.1016/j.envpol.2012.08.002

Cited by 57 publications

(46 citation statements)

References 38 publications

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“…PNP was monitored at 280 nm with an Agilent ultraviolet detector, and its retention time was 11.2 min. The NH 4 + (Fawcett and Scott 1960) and NO 2 − (Lessner et al 2002) concentrations were colorimetrically detected (Chi et al 2013). The concentration of nitrate (NO 3 − ) was determined as described by Norman et al (1985).…”

Section: Soil Microcosm Experimentsmentioning

confidence: 99%

“…Chemical analysis PNP concentration was monitored using high-performance liquid chromatography (HPLC) as described by Chi et al (2013). In detail, PNP was assessed using an Agilent series 1200 system (Agilent Technologies, Palo Alto, CA, USA) equipped with a C 18 reversed-phase column (5 μm, 4.6× 250 mm; Agilent Technologies) maintained at 30°C.…”

Section: Soil Microcosm Experimentsmentioning

confidence: 99%

“…The pnpA gene encodes the enzyme responsible for the initial degradation of PNP in strain WBC-3. The reaction solution and PCR program used for quantification of the pnpA gene were as described previously (Chi et al 2013). The probe, primers, and thermal programs used for total bacterial 16S ribosomal RNA (rRNA) gene quantification in soil samples were as described previously (Chi et al 2013;Shen et al 2008).…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

“…The reaction solution and PCR program used for quantification of the pnpA gene were as described previously (Chi et al 2013). The probe, primers, and thermal programs used for total bacterial 16S ribosomal RNA (rRNA) gene quantification in soil samples were as described previously (Chi et al 2013;Shen et al 2008). The amoA gene of AOB and AOA were quantified as described previously (He et al 2007;Shen et al 2008).…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

“…Four treatment groups were established: (T1) natural soil, (T2) natural soil with PNP added, (T3) natural soil with PNP added and strain WBC-3 (CCTCCM201027) inoculated and, (T4) sterile soil with PNP added. All subsequent settings of different treatments were prepared as described by Chi et al (2013).…”

Section: Soil Microcosm Experimentsmentioning

confidence: 99%

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Effects of bioaugmentation in para-nitrophenol-contaminated soil on the abundance and community structure of ammonia-oxidizing bacteria and archaea

Chi

Liu

Zhou

2015

Appl Microbiol Biotechnol

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Pseudomonas sp. strain WBC-3 mineralizes the priority pollutant para-nitrophenol (PNP) and releases nitrite (NO 2 − ), which is probably involved in the nitrification. In this study, the rate of PNP removal in soil bioaugmented with strain WBC-3 was more accelerated with more NO 2 − accumulation than in uninoculated soils. Strain WBC-3 survived well and remained stable throughout the entire period. Realtime polymerase chain reaction (real-time PCR) indicated a higher abundance of ammonia-oxidizing bacteria (AOB) than ammonia-oxidizing archaea (AOA), suggesting that AOB played a greater role in nitrification in the original sampled soil. Real-time PCR and multivariate analysis based on the denaturing gradient gel electrophoresis showed that PNP contamination did not significantly alter the abundance and community structure of ammonia oxidizers except for inhibiting the AOB abundance. Bioaugmentation of PNP-contaminated soil showed a significant effect on AOB populations and community structure as well as AOA populations. In addition, ammonium (NH 4 + ) variation was found to be the primary factor affecting the AOB community structure, as determined by the correlation between the community structures of ammonia oxidizers and environmental factors. It is here proposed that the balance between archaeal and bacterial ammonia oxidation could be influenced significantly by the variation in NH 4 + levels as caused by bioaugmentation of contaminated soil by a pollutant containing nitrogen.

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