Bioassay-guided isolation of antifungal amides against Sclerotinia sclerotiorum from the seeds of Clausena lansium

Feng, Juntao; Xiong, Zi; Xie, Na; Liu, Shanzhi; Zhang, Lianlian; Fang, Xu; Guo, Wenhui; Feng, Juntao

doi:10.1016/j.indcrop.2018.05.037

Cited by 30 publications

(24 citation statements)

References 41 publications

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“…The tested strains were collected and identified from different areas of China and were preserved in the laboratory of the Research & Development Center of Biorational Pesticide, Northwest A&F University. These strains were also identified and used in the earlier studies [ 7 , 33 , 48 ], and the specific information is as follows:…”

Section: Methodsmentioning

confidence: 99%

“…Oxalic acid content and pH value determination: Oxalic acid content was determined according to the method of a previous study [ 7 ]. Distilled water was used as control.…”

Section: Methodsmentioning

confidence: 99%

“…Currently, chemical control is still the most efficient method to control pathogenic microorganisms; however, extensive drug resistance has developed in pathogenic microbe populations due to unscientific and long-term pesticide usage [ 4 , 5 , 6 ]. Therefore, there is great practical significance and urgent demand for developing new broad-spectrum fungicides [ 7 ]. Traditional Chinese herbal medicinal crops are considered a giant treasure trove in the search for environmentally friendly botanical fungicides with low cost, high efficacy, and easy degradation [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Bioassay-Guided Isolation of Broad-Spectrum Fungicidal Active Compound from Artemisia ordosica

Tang

Yang

et al. 2021

Metabolites

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To avoid the widespread resistance of commercial fungicides, new broad-spectrum botanical fungicides need to be developed. In previous bioactive screening assays, extracts of Artemisia ordosica Krasch. (A. ordosica) had highly antifungal activities, but the responsible phytochemicals were unidentified. In this study, active compounds of A. ordosica extracts were identified using a bioassay-guided method, and antifungal assays were performed in vitro and in vivo. The bioactive compounds were dissolved in petroleum ether, and the best antifungal fraction contained four compounds: trans-dehydromatricaria ester (TDDE), 7, 4-demetylnringenin, capillarin, and stearic acid. Among them, TDDE exhibited the highest antifungal activity against six pathogenic fungi and five bacteria. It exhibited significant fungicidal activity against Thanatephorus cucumeris and Botrytis cinerea with EC50 values of 0.464 μg/mL and 1.4 μg/mL, respectively. The living tissue bioassay results showed that the relative protection effects (RPE) of TDDE on tomato leaves, tomato fruit, and strawberry leaves infected with B. cinerea reached 76.78%, 86.2%, and 80.89%, respectively. In pot experiments, the RPE on tomato and strawberry plants infected with B. cinerea reached 84.11% and 96.37%, respectively. Morphological and physiological examination showed that TDDE had significant inhibitory effects on mycelial growth, including increased top offshoot, contorted hyphal tips, and extravasated cytochylema. Meanwhile, bactericidal activities of TDDE were significantly higher than kanamycin and streptomycin in five bacteria, and the plant tissue experiments further demonstrated that it had an 88.31% RPE on walnut leaves infected with Xanthomonas campestris pv. jugiandis, 72.18% RPE on potato infected with Erwinia carotovora subsp. carotovora, and 82.50% RPE on kiwifruit branches infected with Pseudomonas syringae pv. actinidiae. The active compounds isolated from A. ordosica in this study show great potential value for developing broad-spectrum fungicides, and also provide an important way to identify and isolate new bioactive products from medicinal plants.

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Section: Methodsmentioning

confidence: 99%

“…Oxalic acid content and pH value determination: Oxalic acid content was determined according to the method of a previous study [ 7 ]. Distilled water was used as control.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bioassay-Guided Isolation of Broad-Spectrum Fungicidal Active Compound from Artemisia ordosica

Tang

Yang

et al. 2021

Metabolites

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“…The seeds of wampee were also employed as folk medicine for the treatment of acute and chronic gastro‐intestinal disorders (Shen et al., 2018). Amide alkaloids isolated from the seeds of C. lansium showed potent nematicidal activity against Panagrellus redivivus (Fan et al., 2018) and antifungal effect against Sclerotinia sclerotiorum (Yan et al., 2018). Comparative assessment of phytochemical profiles and antioxidant activities in fruits of five varieties of wampee was conducted to demonstrate the difference of total phenolics, flavonoids, and antioxidant activities among different cultivars (Chang, Lu, et al, 2018; Chang, Ye, et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

A comparative UPLC‐Q‐Orbitrap‐MS untargeted metabolomics investigation of different parts of Clausena lansium (Lour.) Skeels

Fan

Peng

Zhang

et al. 2020

Food Science & Nutrition

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“…C. lansium is famous for their fruits, which are usually very popular tropical, health-promoting fruits, while their roots, stems, leaves, and seeds have also been extensively applied in folk medicine or traditional Chinese medicine for the treatments of abdominal pain, malaria, cold, dermatopathy, and snake bites [2,3]. Various biological studies, on the alkaloids, coumarins, and sesquiterpenes from this plant have reported the neuroprotective [4,5], antitumor [6,7], hepatoprotective [8], anti-inflammatory [9], antifungal [10], antioxidant [11], antiobesity [12], nematicidal [13], antimicrobial [14], and hypoglycemic [15] effects of the C. lansium . In our previous studies, some carbazole alkaloids [16] and coumarins [17] were separated from the stem and leaf of C. lansium .…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Study of Stem and Leaf of Clausena lansium

Peng

et al. 2019

Molecules

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Clausena lansium Lour. Skeels (Rutaceae) is widely distributed in South China and has historically been used as a traditional medicine in local healthcare systems. Although the characteristic components (carbazole alkaloids and coumarins) of C. lansium have been found to possess a wide variety of biological activities, little attention has been paid toward the other components of this plant. In the current study, phytochemical analysis of isolates from a water-soluble stem and leaf extract of C. lansium led to the identification of 12 compounds, including five aromatic glycosides, four sesquiterpene glycosides, two dihydrofuranocoumarin glycosides, and one adenosine. All compounds were isolated for the first time from the genus Clausena, including a new aromatic glycoside (1), a new dihydrofuranocoumarin glycoside (6), and two new sesquiterpene glycosides (8 and 9). The phytochemical structures of the isolates were elucidated using spectroscopic analyses including NMR and MS. The existence of these compounds demonstrates the taxonomic significance of C. lansium in the genus Clausena and suggests that some glycosides from this plant probably play a role in the anticancer activity of C. lansium to some extent.

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Bioassay-guided isolation of antifungal amides against Sclerotinia sclerotiorum from the seeds of Clausena lansium

Cited by 30 publications

References 41 publications

Bioassay-Guided Isolation of Broad-Spectrum Fungicidal Active Compound from Artemisia ordosica

Bioassay-Guided Isolation of Broad-Spectrum Fungicidal Active Compound from Artemisia ordosica

A comparative UPLC‐Q‐Orbitrap‐MS untargeted metabolomics investigation of different parts of Clausena lansium (Lour.) Skeels

Phytochemical Study of Stem and Leaf of Clausena lansium

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