Bioaerosol Sampling with a Wetted Wall Cyclone: Cell Culturability and DNA Integrity of<i>Escherichia coli</i>Bacteria

King, Maria D.; McFarland, Andrew R.

doi:10.1080/02786826.2011.605400

Cited by 53 publications

(47 citation statements)

References 39 publications

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“…Stewart et al observed that 49% of Pseudomonas fluorescens bacteria lost their culturability after impacting an agar surface at a speed of 40 m/s, most likely due to mechanical stress (19). Another study showed that the intactness of the genomic DNA was impaired due to the stress of impaction onto the collection surface (20). Recently, found that sampling stress from a variety of bioaerosol samplers decreased the bacterial culturability (21,22).…”

mentioning

confidence: 99%

Release of Free DNA by Membrane-Impaired Bacterial Aerosols Due to Aerosolization and Air Sampling

Zhen

Han

Fennell

et al. 2013

Appl Environ Microbiol

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We report here that stress experienced by bacteria due to aerosolization and air sampling can result in severe membrane impairment, leading to the release of DNA as free molecules. Escherichia coli and Bacillus atrophaeus bacteria were aerosolized and then either collected directly into liquid or collected using other collection media and then transferred into liquid. The amount of DNA released was quantified as the cell membrane damage index (I D )

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mentioning

confidence: 99%

Release of Free DNA by Membrane-Impaired Bacterial Aerosols Due to Aerosolization and Air Sampling

Zhen

Han

Fennell

et al. 2013

Appl Environ Microbiol

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“…Three collecting systems (namely solid impaction, liquid impingement and filtration) were tested for collection of aerosolized Legionella detected using fluorescent in situ hybridization -FISH 9 . The cyclone-based samplers were tested and the physical sampling efficiency was evaluated for several types 10,11 . Pan et al developed aerosol sorter based on fluorescence spectrum of the aerosol that provides the cue whether to deflect that aerosol particle by puffing air stream 12 .…”

Section: Introductionmentioning

confidence: 99%

Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms

Kovar

Skládal

2015

SPIE Proceedings

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Biological warfare agents (BWAs) represent the current menace of the asymmetric war. The early detection of BWAs, especially in the form of bioaerosol, is a challenging task for governments all around the world. Label-free quartz crystal microbalance (QCM) immunosensor and electrochemical immunosensor were developed and tested for rapid detection of BWA surrogate (E. coli) in the form of bioaerosol. Two immobilization strategies for the attachment of antibody were tested; the gold sensor surface was activated by cysteamine and then antibody was covalently linked either using glutaraldehyde, or the reduced antibodies were attached via Sulfo-SMCC. A portable bioaerosol chamber was constructed and used for safe manipulation with aerosolized microorganisms. The dissemination was done using a piezoelectric humidifier, distribution of bioaerosol inside the chamber was ensured using three 12-cm fans. The whole system was controlled remotely using LAN network. The disseminated microbial cells were collected and preconcentrated using the wetted-wall cyclone SASS 2300, the analysis was done using the on-line linked immunosensors. The QCM immunosensor had limit of detection 1×10 4 CFU·L −1 of air with analysis time 16 min, the whole experiment including dissemination and sensor surface regeneration took 40 min. In case of blank (disseminated sterile buffer), no signal change was observed. The electrochemical immunosensor was able to detect 150 CFU·L −1 of air in 20 min; also in this case, no interferences were observed. Reference measurements were done using particle counter Met One 3400 and by cultivation method on agar plates. The sensors have proved to be applicable for rapid screening of microorganisms in air.

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“…In parallel, the compatibility of collection fluids with preservation of biological material and downstream analytical methods also need to be addressed. Indeed, collection in fluids with unfavorable ionic or glycerol contents could result in decreased viability of microorganisms, ruptured cell membranes, and ultimately in the degradation of DNA, due to osmotic stress (Chang and Chou 2011;King and McFarland 2012). As an example, the user's manual advises that ViaTrap mineral oil may not be suitable for polymerase chain reaction (PCR) analysis.…”

Section: 34mentioning

confidence: 99%

Physical performances and kinetics of evaporation of the CIP 10-M personal sampler's rotating cup containing aqueous or viscous collection fluid

Simon

Bau

Boivin

et al. 2016

Aerosol Science and Technology

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The CIP 10-M personal sampler measures worker exposure to airborne particles by collecting particles in a rotating metal cup containing a few milliliters of a collection fluid. This device is mainly used to sample microorganisms or microbial components to measure bioaerosol concentrations in various occupational environments. Aqueous liquids are generally used, but their rapid evaporation limits the duration of sampling; alternative collection fluids could alleviate this problem. Indeed, the particle-collection efficiency of the rotating cup has not been extensively studied, and the only data available relate to a discontinued model. This study aimed to measure the collection efficiency of the current rotating cup model containing an aqueous (water) or viscous (ViaTrap mineral oil) collection fluid. The kinetics of evaporation confirmed that ViaTrap does not evaporate, making 8-h sampling campaigns in constant volumes feasible. Particles with a wide range of aerodynamic diameters (between around 0.1 and 10 mm) were produced using various test rigs and mono-or polydisperse test aerosols. Both new and older cup models performed similarly, with a collection efficiency of >80% for larger particles (aerodynamic diameters >2.8 mm), progressively decreasing to around 50% for aerodynamic diameters of 2.1 mm; with aerodynamic diameters of <1 mm, the collection efficiency was generally <10%. In physical terms, collection efficiency was unaffected by the type (aqueous or viscous) or volume (between 0 and 3 mL) of collection fluid used. Bias maps indicated that the inhalable fraction may be underestimated in occupational settings, particularly with aerosols mainly composed of particles with aerodynamic diameters of less than around 3 mm. EDITORTiina Reponen

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Bioaerosol Sampling with a Wetted Wall Cyclone: Cell Culturability and DNA Integrity ofEscherichia coliBacteria

Cited by 53 publications

References 39 publications

Release of Free DNA by Membrane-Impaired Bacterial Aerosols Due to Aerosolization and Air Sampling

Release of Free DNA by Membrane-Impaired Bacterial Aerosols Due to Aerosolization and Air Sampling

Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms

Physical performances and kinetics of evaporation of the CIP 10-M personal sampler's rotating cup containing aqueous or viscous collection fluid

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