Bioactivity of Khail-khail (Elaeagnus latifolia) Leaf Extract to Bacteria Staphylococcus saprophyticus and Salmonella enterica

Roza, Destria; Sutiani, Ani; Meilano, Rendi

doi:10.4108/eai.18-10-2018.2287381

Cited by 1 publication

(4 citation statements)

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“…The macerated ethanol extract is combined and filtered. The obtained filtrate was evaporated using a rotary evaporator at low pressure, resulting in ethanol extract [10]…”

Section: Methodsmentioning

confidence: 99%

“…The suspension of bacteria into 0.9% NaCl is the same as bacterial dilution of 10-6 CFU/mL (McFarland Standard 0.5). [10][11]…”

Section: Bacterial Suspensionmentioning

confidence: 99%

“…The resulting fraction was then monitored by TLC with the n-hexane mobile phase : ethylacetate for eluent test. The same Rf values then be grouped into several combined fractions, which are then tested for phytochemicals again [10].…”

Section: A Separation With Vlc and Tlcmentioning

confidence: 99%

“…The same Rf value was combined and then one of the fractions was selected based on the incorporation of the Rf value in the TLC with the three-eluent test namely n-hexane: ethyl acetate (1:1), n-hexane: chloroform (1:1) and ethyl acetate: chloroform (1:1) until it indicates a single spot. [10].…”

Section: B Separation By Gravity Column Chromatographymentioning

confidence: 99%

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Anti-bacterial activity of ethanol extract of the stem of coffee parasite (Scurulla ferruginea (Robx. Ex Jack) Danser) and its secondary metabolite isolation

Roza¹,

Indriani²,

Wiliranti³

et al. 2022

The 8th Annual International Seminar on Trends in Science and Science Education (Aistsse) 2021

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The aim of this study is to determine the antibacterial activity of the ethanol extract of the stem of the coffee parasite (Scurulla ferruginea (Robx. Ex Jack) Danser) using disc diffusion and microdilution methods. In this study, secondary metabolites were isolated from coffee parasite stems using Vacuum Liquid Chromatography and Column Chromatography continued by characterized the compounds using GC-MS (Gas chromatography-mass spectroscopy). Based on the identification using GC-MS, it was known that the stem of the coffee parasite contains diisooctyl phthalate. The results of the antibacterial activity test of the disc diffusion method with an extract concentration of 1% against Staphylococcus aureus, Salmonella typhi, Streptcoccus viridans, Escherichia coli, Streptococcus mutans, and Bacillus aureus bacteria were 6.35 mm, 6.7 mm, 8.95 mm, 7 mm, 8.75 mm and 8.2 mm respectively. This result shows that the ability to inhibit bacterial growth of 1% extract is in the medium category. The MIC values for Escherichia coli, Streptococcus mutans, and Bacillus aureus bacteria were 2500 g/mL, while the MIC values for Staphylococcus aureus, Staphylococcus typhi, Streptcoccus viridans were 5000 g/mL. The MBC value of Streptcoccus viridans, Streptococcus mutans, and Bacillus aureus bacteria was greater than 5000 g/mL, only Escherichia coli bacteria can be killed at a concentration of 5000 g/mL and Salmonella typhi at a concentration of 2500 g/mL. The test results showed that the stem extract of Scurulla ferruginea was only as an inhibitor.

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“…The macerated ethanol extract is combined and filtered. The obtained filtrate was evaporated using a rotary evaporator at low pressure, resulting in ethanol extract [10]…”

Section: Methodsmentioning

confidence: 99%

“…The suspension of bacteria into 0.9% NaCl is the same as bacterial dilution of 10-6 CFU/mL (McFarland Standard 0.5). [10][11]…”

Section: Bacterial Suspensionmentioning

confidence: 99%

Section: A Separation With Vlc and Tlcmentioning

confidence: 99%

Section: B Separation By Gravity Column Chromatographymentioning

confidence: 99%

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