Bioactive Glass Foam Scaffolds are Remodelled by Osteoclasts and Support the Formation of Mineralized Matrix and Vascular Networks In Vitro

Midha, Swati; Bergh, Wouter van den; Kim, Taek B.; Lee, Peter; Jones, Julian R.; Mitchell, Christopher A.

doi:10.1002/adhm.201200140

Cited by 55 publications

(48 citation statements)

References 43 publications

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“…5.6 m 2 g À1 compared to 0.15 m 2 g À1 for 45S5 particles [37] in a similar particle size range to those used by Oonishi [36]) and dissolution rate for bone remodelling. Ripamonti et al [38] showed that concave pores could encourage more rapid osteogenesis and osteoclast remodelling of bioactive glasses has been observed in vitro [39]. Further experiments are required to verify this hypothesis, using histology and quantitative methods.…”

Section: In Vivo Studymentioning

confidence: 99%

Highly degradable porous melt-derived bioactive glass foam scaffolds for bone regeneration

et al. 2017

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a b s t r a c tA challenge in using bioactive melt-derived glass in bone regeneration is to produce scaffolds with interconnected pores while maintaining the amorphous nature of the glass and its associated bioactivity. Here we introduce a method for creating porous melt-derived bioactive glass foam scaffolds with low silica content and report in vitro and preliminary in vivo data. The gel-cast foaming process was adapted, employing temperature controlled gelation of gelatin, rather than the in situ acrylic polymerisation used previously. To form a 3D construct from melt derived glasses, particles must be fused via thermal processing, termed sintering. The original Bioglass Ò 45S5 composition crystallises upon sintering, altering its bioactivity, due to the temperature difference between the glass transition temperature and the crystallisation onset being small. Here, we optimised and compared scaffolds from three glass compositions, ICIE16, PSrBG and 13-93, which were selected due to their widened sintering windows. Amorphous scaffolds with modal pore interconnect diameters between 100-150 mm and porosities of 75% had compressive strengths of 3.4 ± 0.3 MPa, 8.4 ± 0.8 MPa and 15.3 ± 1.8 MPa, for ICIE16, PSrBG and 13-93 respectively. These porosities and compressive strength values are within the range of cancellous bone, and greater than previously reported foamed scaffolds. Dental pulp stem cells attached to the scaffold surfaces during in vitro culture and were viable. In vivo, the scaffolds were found to regenerate bone in a rabbit model according to X-ray micro tomography imaging. Statement of SignificanceThis manuscript describes a new method for making scaffolds from bioactive glasses using highly bioactive glass compositions. The glass compositions have lower silica content that those that have been previously made into amorphous scaffolds and they have been designed to have similar network connectivity to that of the original (and commercially used) 45S5 Bioglass. The aim was to match Bioglass' bioactivity. The scaffolds retain the amorphous nature of bioactive glass while having an open pore structure and compressive strength similar to porous bone (the original 45S5 Bioglass crystallises during sintering, which can cause reduced bioactivity or instability).The new scaffolds showed unexpectedly rapid bone regeneration in a rabbit model.

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Section: In Vivo Studymentioning

confidence: 99%

Highly degradable porous melt-derived bioactive glass foam scaffolds for bone regeneration

et al. 2017

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“…The objectives of this paper were to: i) Revisit the original Stöber process to provide a comprehensive understanding of how to reliably control particle size, whilst maintaining monodispersed particles; ii) Investigate the subsequent incorporation of calcium with complete quantitative characterisation of the nanoparticles to prove the incorporation of calcium, with the 70S30C composition (70 mol% SiO2, 30 mol% CaO) [32][33][34] as the target value; iii) Assess the dissolution of the particles in artificial lysosomal fluid (ALF) and Simulated Body Fluid (SBF) to determine how they might degrade in the body.…”

Section: Introductionmentioning

confidence: 99%

Controlling particle size in the Stöber process and incorporation of calcium

Greasley

Page

Sirovica

et al. 2016

Journal of Colloid and Interface Science

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143

154

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The Stӧber process is commonly used for synthesising spherical silica particles. This article reports the first comprehensive study of how the process variables can be used to obtain monodispersed particles of specific size. The modal particle size could be selected within in the range 20 -500 nm. There is great therapeutic potential for bioactive glass nanoparticles, as they can be internalised within cells and perform sustained delivery of active ions. Biodegradable bioactive glass nanoparticles are also used in nanocomposites. Modification of the Stӧber process so that the particles can contain cations such as calcium, while maintaining monodispersity, is desirable. Here, while calcium incorporation is achieved, with a homogenous distribution, careful characterisation shows that much of the calcium is not incorporated. A maximum of 10 mol% CaO can be achieved and previous reports are likely to have overestimated the amount of calcium incorporated.

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“…Gelatin was used in this system as it is a hydrolysed form of collagen which is recognisable and adhered to by many cell types [22]. And silica is well documented in biocompatible applications [23][24][25]. Consequently silica-gelatin hybrids also display excellent cell compatibility in vitro with mesenchymal stem cells [9].…”

Section: Introductionmentioning

confidence: 99%

Silica–gelatin hybrids for tissue regeneration: inter-relationships between the process variables

Mahony

Sheng

Turdean-Ionescu

et al. 2013

J Sol-Gel Sci Technol

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Owing to their diverse range of highly tailorable material properties, inorganic/organic hybrids have the potential to meet the needs of biodegradable porous scaffolds across a range of tissue engineering applications. One such hybrid platform, the silica-gelatin sol-gel system, was examined and developed in this study. These hybrid scaffolds exhibit covalently linked interpenetrating networks of organic and inorganic components, which allows for independent control over their mechanical and degradation properties. A combination of the sol-gel foaming process and freeze drying was used to create an interconnected pore network. The synthesis and processing of the scaffolds has many variables that affect their structure and properties. The focus of this study was to develop a matrix tool that shows the inter-relationship between process variables by correlating the key hybrid material properties with the synthesis parameters that govern them. This was achieved by investigating the effect of the organic (gelatin) molecular weight and collating previously reported data.Control of molecular weight of the polymer is as an avenue that allows the modification of hybrid material properties without changing the surface chemistry of the material, which is a factor that governs the cell and tissue interaction with the scaffold. This presents a significant step forward in understanding the complete potential of the silica-gelatin hybrid system as a medical device.

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Bioactive Glass Foam Scaffolds are Remodelled by Osteoclasts and Support the Formation of Mineralized Matrix and Vascular Networks In Vitro

Cited by 55 publications

References 43 publications

Highly degradable porous melt-derived bioactive glass foam scaffolds for bone regeneration

Highly degradable porous melt-derived bioactive glass foam scaffolds for bone regeneration

Controlling particle size in the Stöber process and incorporation of calcium

Silica–gelatin hybrids for tissue regeneration: inter-relationships between the process variables

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