Bioactive doped metals: high synergism in the bactericidal activity of chlorhexidine@silver towards wound pathogenic bacteria

Ben-Knaz, Racheli; Pedahzur, Rami; Avnir, David

doi:10.1039/c3ra41196f

Cited by 28 publications

(24 citation statements)

References 38 publications

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“…Hence, there is an urgent need for new approaches to overcome antibiotic resistance and to develop alternate antimicrobial agents that can control infectious diseases, the spread of pathogens and which have long-term effectiveness [3]. One area to prolong antimicrobial activity is with the incorporation of antimicrobial agents within sustained-release delivery systems for their continuous use [4][5][6]. The objective of using new sustained-release drug delivery systems is to enhance the therapeutic effectiveness of drugs, by decreasing their side effects and increasing their bioavailability in certain sites.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Silver-Treated Bacteria against other Multi-Drug Resistant Pathogens in Their Environment

et al. 2020

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Silver is a potent antimicrobial agent against a variety of microorganisms and once the element has entered the bacterial cell, it accumulates as silver nanoparticles with large surface area causing cell death. At the same time, the bacterial cell becomes a reservoir for silver. This study aims to test the microcidal effect of silver-killed E. coli O104: H4 and its supernatant against fresh viable cells of the same bacterium and some other species, including E. coli O157: H7, Multidrug Resistant (MDR) Pseudomonas aeruginosa and Methicillin Resistant Staphylococcus aureus (MRSA). Silver-killed bacteria were examined by Transmission Electron Microscopy (TEM). Agar well diffusion assay was used to test the antimicrobial efficacy and durability of both pellet suspension and supernatant of silver-killed E. coli O104:H4 against other bacteria. Both silver-killed bacteria and supernatant showed prolonged antimicrobial activity against the tested strains that extended to 40 days. The presence of adsorbed silver nanoparticles on the bacterial cell and inside the cells was verified by TEM. Silver-killed bacteria serve as an efficient sustained release reservoir for exporting the lethal silver cations. This promotes its use as a powerful disinfectant for polluted water and as an effective antibacterial which can be included in wound and burn dressings to overcome the problem of wound contamination.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Silver-Treated Bacteria against other Multi-Drug Resistant Pathogens in Their Environment

et al. 2020

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“…13,[18][19][20] Importantly, the combination of CHX and Ag NPs has a synergistic antibacterial effect and improved biofilm inhibitory activity. 21,22 However, side effects occurring from the long-term coadministration without targeting the biofilm microenvironment may limit its further clinical applications. 23 In this regard, a promising drug co-delivery system is highly desirable for the controlled release of CHX and silver ions in response to a pathological environment in the oral cavity.…”

Section: Introductionmentioning

confidence: 99%

Redox/pH dual-controlled release of chlorhexidine and silver ions from biodegradable mesoporous silica nanoparticles against oral biofilms

Lu¹,

Ge²,

Qiu³

et al. 2018

IJN

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BackgroundOral plaque biofilms pose a threat to periodontal health and are challenging to eradicate. There is a growing belief that a combination of silver nanoparticles and chlorhexidine (CHX) is a promising strategy against oral biofilms.PurposeTo overcome the side effects of this strategy and to exert maximum efficiency, we fabricated biodegradable disulfide-bridged mesoporous silica nanoparticles (MSNs) to co-deliver silver nanoparticles and CHX for biofilm inhibition.Materials and methodsCHX-loaded, silver-decorated mesoporous silica nanoparticles (Ag-MSNs@CHX) were fabricated after CHX loading, and the pH- and glutathione-responsive release profiles of CHX and silver ions along with their mechanism of degradation were systematically investigated. Then, the efficacy of Ag-MSNs@CHX against Streptococcus mutans and its biofilm was comprehensively assessed by determining the minimum inhibitory concentration, minimum bactericidal concentration, minimal biofilm inhibitory concentration, and the inhibitory effect on S. mutans biofilm formation. In addition, the biosafety of nanocarriers was evaluated by oral epithelial cells and a mouse model.ResultsThe obtained Ag-MSNs@CHX possessed redox/pH-responsive release properties of CHX and silver ions, which may be attributed to the redox-triggered matrix degradation mechanism of exposure to biofilm-mimetic microenvironments. Ag-MSNs@CHX displayed dose-dependent antibacterial activity against planktonic and clone formation of S. mutans. Importantly, Ag-MSNs@CHX had an increased and long-term ability to restrict the growth of S. mutans biofilms compared to free CHX. Moreover, Ag-MSNs@CHX showed less cytotoxicity to oral epithelial cells, whereas orally administered Ag-MSNs exhibited no obvious toxic effects in mice.ConclusionOur findings constitute a highly effective and safe strategy against biofilms that has a good potential as an oral biofilm therapy.

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“…15,16 Importantly, recent studies demonstrated that the combination of CHX and silver ions could engender an obviously synergistic bactericidal effect and improve the bactericidal efficacy. 17, 18 Monteiro et al 17 have demonstrated that silver nanoparticles (NPs) in combination with CHX may disrupt Candida biofilms, resulting in the prevention of resistance and improving the treatment of Candida-associated denture stomatitis. Ben-Knaz et al 18 fabricated…”

Section: Introductionmentioning

confidence: 99%

“…17, 18 Monteiro et al 17 have demonstrated that silver nanoparticles (NPs) in combination with CHX may disrupt Candida biofilms, resulting in the prevention of resistance and improving the treatment of Candida-associated denture stomatitis. Ben-Knaz et al 18 fabricated…”

Section: Introductionmentioning

confidence: 99%

Synergistic bactericidal activity of chlorhexidine-loaded, silver-decorated mesoporous silica nanoparticles

Lu¹,

Wang²,

Chang³

et al. 2017

IJN

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Combination of chlorhexidine (CHX) and silver ions could engender synergistic bactericidal effect and improve the bactericidal efficacy. It is highly desired to develop an efficient carrier for the antiseptics codelivery targeting infection foci with acidic microenvironment. In this work, monodisperse mesoporous silica nanoparticle (MSN) nanospheres were successfully developed as an ideal carrier for CHX and nanosilver codelivery through a facile and environmentally friendly method. The CHX-loaded, silver-decorated mesoporous silica nanoparticles (Ag-MSNs@CHX) exhibited a pH-responsive release manner of CHX and silver ions simultaneously, leading to synergistically antibacterial effect against both gram-positive Staphylococcus aureus and gram-negative Escherichia coli . Moreover, the effective antibacterial concentration of Ag-MSNs@CHX showed less cytotoxicity on normal cells. Given their synergistically bactericidal ability and good biocompatibility, these nanoantiseptics might have effective and broad clinical applications for bacterial infections.

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Bioactive doped metals: high synergism in the bactericidal activity of chlorhexidine@silver towards wound pathogenic bacteria

Cited by 28 publications

References 38 publications

Antimicrobial Activity of Silver-Treated Bacteria against other Multi-Drug Resistant Pathogens in Their Environment

Antimicrobial Activity of Silver-Treated Bacteria against other Multi-Drug Resistant Pathogens in Their Environment

Redox/pH dual-controlled release of chlorhexidine and silver ions from biodegradable mesoporous silica nanoparticles against oral biofilms

Synergistic bactericidal activity of chlorhexidine-loaded, silver-decorated mesoporous silica nanoparticles

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