Bio-speckle activity applied to the assessment of tomato fruit ripening

Romero, Gladis Graciela; Martínez, Carlos Córdova; Alanís, Elvio; Salazar, Germán Ariel; Broglia, Viviana; Álvarez, L.

doi:10.1016/j.biosystemseng.2009.02.001

Cited by 36 publications

(19 citation statements)

References 8 publications

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“…Apples that suffered mechanical injury showed a decline in activity at the location where the physical damage occurred (Pajuelo et al, 2003). According to Romero et al (2009), the biological activity of tomato fruits increased as they matured, while another study showed that a decrease in chlorophyll content was accompanied by increased biological activity in apple fruits (Zdunek and Herppich,2012).…”

Section: Discussionmentioning

confidence: 99%

Effects of silicon on antioxidant enzymes, CO2, proline and biological activity of in vitro-grown cape gooseberry under salinity stress

Rezende¹,

Soares²,

Santos³

et al. 2017

Aust J Crop Sci

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Cape gooseberry (Physalis peruviana L.) cannot tolerate high levels of salinity. Salt stress is one of the most damaging abiotic stresses that affects plant development. However, there are some evidences that silicon (Si) can act as a mitigating agent of environmental stresses. Towards understanding the stress using tissue culture, we investigated the effect of in vitro NaCl-induced salt stress in cape gooseberry and the potential mitigating effect provided by Si. Nodal segments were inoculated in MS medium with different combinations of NaCl concentrations (0.5 and 1.0% NaCl) and silicic acid (0.0, 0.5, and 1.0 g L -1 ). The experimental design was completely randomized, in a factorial design with a control treatment [(2x3) + 1], totalizing 7 treatments. The control treatment consisted of an additional treatment without the addition of any of the factors. Isoenzyme activity, proline content, CO 2 level and biological activity were assessed after 30 d. We found an increase in all antioxidant enzymes (SOD, CAT and POD) activities when salt stress was imposed, indicating that the plant has an efficient system to protect itself against stress. Among these enzymes, Si played a mitigating role only in POD activity. In relation to other evaluated enzymes, NaCl enhanced the EST and MDH activities, followed by a subsequent decrease in MDH. Si showed different performances according to the concentration of NaCl. Electrophoretic analysis represented by bands illustrated these behaviors. Proline content increased as salinity increased, and Si effect was observed by increasing the levels of this amino acid. High CO 2 level was found at the concentration of 1.0% NaCl, and Si contributed to decrease this variable to normal levels. The reduction in the respiratory rates may contribute to the allocation of carbon to other chemical reactions such as the synthesis of new tissues. Salt stress also increased the biological activity of leaves and there was no effect of Si for this variable. We conclude that salt stress causes a great damage to in vitro-grown cape gooseberry plants and the addition of 1.0 g L -1 Si can ameliorate that damage for some characteristics. However, more studies are necessary since the physiological changes played by Si have been poorly understood for dicots.

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Section: Discussionmentioning

confidence: 99%

Effects of silicon on antioxidant enzymes, CO2, proline and biological activity of in vitro-grown cape gooseberry under salinity stress

Rezende¹,

Soares²,

Santos³

et al. 2017

Aust J Crop Sci

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“…LSI has been developed for many purposes in assessing fruits quality and ripeness, the quality of orange fruits [6], the attribute quality of apples [7], and the ripeness of tomato [8]. Laser speckles are obtained when a diffuse surface illuminated by laser light.…”

Section: Introductionmentioning

confidence: 99%

Experiment on Laser Speckle Imaging of Apples Using A CMOS Camera

Shiddiq¹

2016

KEG

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Laser Speckle Imaging recently has become a promising tool to assess fruit quality and can be applied in fruit sorting. It is a non-destructive, optical method which uses light scattered of fruit surface after illuminated by laser light. Laser speckle imaging methods use He-Ne laser or diode laser as the light source yet both lasers have differences in performance and price. Diode lasers are more preferable due to their efficiency, low cost, small and compact, and varied wavelength. In this research, an optical system which consisted of a laser source and a CMOS camera was used for Laser Speckle Imaging. A 632,8 nm He-Ne laser and a 650 nm diode laser were used, their performances were compared. The samples were two kinds of apples, Red Delicious and Fuji, five samples for each cultivar. The laser light was expanded using a beam expander hence illuminated on the apple surface at 30 angle. A monochrome Thorlabs CMOS camera with camera lens was used to record the speckle patterns of the apple surface. Both lasers were kept at the same laser power by Neutral Density Filters. ImageJ software was used to calculate the gray value of the speckle pattern for each sample, the speckle gray values were compared for different laser light sources and apple types. The results showed that there is a significant difference in gray value level between both apples. Higher maximum gray values were found on the Fuji apples compared to the Red Delicious apples which were about 21.7 % when using He-Ne laser and 18.3 % when using diode laser. Higher maximum gray value for Fuji apples could be due to their rounder shape, firmer skin and flesh, they scatter more light. The curvature and the firmness of the fruits affected the gray value level. For each apple type, there was a slight difference in maximum gray values for both laser sources. Higher gray values were obtained when using diode laser compared to the He-Ne laser, 22.0 % difference for Fuji apples and 25.2 % difference for Red Delicious apples. These could be because of the less coherence, wider bandwidth, and irregular beam shape of the diode laser that it scatters more light and suppresses speckles.

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“…where bi being associated with statistical cummulants [10] and are used as a measure of the speckle activity.…”

Section: A Bio-speckle Techniquesmentioning

confidence: 99%

“…A well-defined and widely used parameter in literature, such as the mean life time of speckle grains [10] was tested. This numerical approach, the most popular for measuring motility of scatters in biological and non biological objects, briefly consists in the approximation of the autocorrelation function applied in a time history of the speckle pattern (THSP) by means of statistical cummulants.…”

Section: A Bio-speckle Techniquesmentioning

confidence: 99%

“…Speckle activity has been extensively studied and as results of several research projects in Optics a wide variety of parameters and methodologies for measuring the activity of such kind of speckle patterns have been proposed. In literature a wide range of applications are reported, from industrial applications like paint drying monitoring [1][2][3] to physiological activity of living objects such as blood flow, heartbeat, viability of seeds, maturity and bruising in fruits among others [4][5][6][7][8][9][10]. The metrological uses of dynamic speckle well consolidated by systematic testing of all the approaches arising by the many applications [11] suggest that it can be used as a tool for monitoring cultivation of microalgae in laboratory scale photobioreactor.…”

Section: Introductionmentioning

confidence: 99%

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Bio-speckle for microalgae growth monitoring in laboratory scale photobioreactor

Romero¹,

Monaldi²,

Domínguez³

et al. 2017

Opt. Pura Apl.

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ABSTRACT:Microalgae are widely used in different industries -pharmaceutical, cosmetic, in those dealing with pollution prevention, aquaculture and especially biofuels. Because of the wide range of use, there is an active interest in microalgae large-scale cultivation. Standard techniques for algae biomass production requires cultivation in nutritious culture media, and well-defined external parameters, such as controlled conditions of light intensity, temperature and aeration gas. Commercial production of microalgae is expensive, and the addition of nutrients in culture media contributes to its high cost. The evaluation of the quality of culture media using wastewater effluents or agro-industrial waste can be a way to reduce algal cultures costs. This research aims to develop a noninvasive method for the testing of effectiveness of agro-industrial waste, previously treated, as culture media through daily monitoring of population increase microalgae. We found that it is possible to detect the growth of the microorganisms by using statistical cumulants as numerical approaches for analyzing the dynamic speckle patterns produced by samples of Chrorella Vulgaris algae cultivated in bold basal culture medium.Key words: Biospleckle, microalgae growing, speckle activity, autocorrelation function, optical density. RESUMEN:Las microalgas, son ampliamente utilizadas en las industrias farmacéutica, cosmética, en aquellas relacionadas con la prevención de contaminación, acuacultura y especialmente en biocombustibles. Debido a su amplio rango de aplicaciones existe un gran interés en el cultivo de microalgas a gran escala. Las técnicas estándar para la producción de biomasa algal requieren de su cultivo en medios nutritivos y parámetros externos bien definidos tales como condiciones controladas de iluminación, temperatura y aireación. La producción comercial de microalgas es costosa y la adición de nutrientes a los medios de cultivos contribuye a aumentar el costo. La evaluación de la calidad de medios de cultivos usando aguas residuales o residuos agroindustriales puede resultar en una vía para reducir costos. Este trabajo tiene por objetivo el desarrollo de un método no invasivo para monitorear la eficiencia de residuos agroindustriales, previamente tratados, como posibles medios de cultivos a través del monitoreo diario del crecimiento poblacional de las microalgas. Hemos encontrado que es posible detectar el crecimiento de los microorganismos usando cumulantes estadísticos como una aproximación numérica para analizar la actividad de patrones de speckle dinámico producidos por muestras de algas Chrorella Vulgaris cultivadas en medio basal Bold.Palabras clave: Biospleckle, crecimiento de microalgas, actividad de speckle, función de autocorrelación, densidad óptica. REFERENCES AND LINKS / REFERENCIAS Y ENLACES[1] H. Rabal, M. Trivi, R. Arizaga, N. Cap, G.Romero, E. Alanís, "Transient phenomena analysis using dynamics speckle patterns," Opt. Eng. 38(1), 57-62 (1996).https://doi.org/10.1117/1.600789[2] G. Romero, E. Alanís, H...

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Bio-speckle activity applied to the assessment of tomato fruit ripening

Cited by 36 publications

References 8 publications

Effects of silicon on antioxidant enzymes, CO2, proline and biological activity of in vitro-grown cape gooseberry under salinity stress

Effects of silicon on antioxidant enzymes, CO2, proline and biological activity of in vitro-grown cape gooseberry under salinity stress

Experiment on Laser Speckle Imaging of Apples Using A CMOS Camera

Bio-speckle for microalgae growth monitoring in laboratory scale photobioreactor

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