Bio-guided identification of proteins for the diagnosis of cysticercosis in swine

Nativel, Priscilla; Rahantamalala, Anjanirina; Ramiandrisoa, Sitraka; Rasoamampianinaa, Virginie; Duchateau, Magalie; Chamot‐Rooke, Julia; Guebey, Remy; Rasamoelina-Andriamanivo, Harentsoaniaina; Jambou, Ronan

doi:10.1016/j.vetpar.2016.02.021

Cited by 5 publications

(5 citation statements)

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“…130 times by the LWs compared to the SWs after three hours of incubation. A Sj-Ts4-like homolog has also been identified among the E/S proteins produced by H. diminuta adult worms ( 41 ) and pig tapeworm, Taenia solium ( 43 ). Initially, the Sj-Ts4 protein was recovered as a novel 23-kDa antigenic protein of blood fluke, S. japonicum ( 44 ).…”

Section: Discussionmentioning

confidence: 99%

Label-free quantitative proteomics and immunoblotting identifies immunoreactive and other excretory-secretory (E/S) proteins of Anoplocephala perfoliata

et al. 2022

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Anoplocephala perfoliata is a common tapeworm in horses causing colic and even mortalities. Current diagnostic tests to detect A. perfoliata infections have their limitations and an improved method is needed. Immunoreactive excretory/secretory proteins (E/S proteome) of this parasite can provide promising candidates for diagnostic tests. We compared E/S proteins produced by small (length < 20 mm, width < 5 mm) and large (length 20 to 40 mm, width 5 to 10 mm) A. perfoliata worms in vitro by label-free quantitative proteomics using a database composed of related Hymenolepis diminuta, Echinococcus multilocularis/granulosus and Taenia aseatica proteins for protein identifications. Altogether, 509 E/S proteins were identified after incubating the worms in vitro for three and eight hours. The greatest E/S proteome changes suggested both worm size- and time-dependent changes in cytoskeleton remodeling, apoptosis, and production of antigens/immunogens. The E/S proteins collected at the three-hour time point represented the natural conditions better than those collected at the eight-hour time point, and thereby contained the most relevant diagnostic targets. Immunoblotting using antibodies from horses tested positive/negative for A. perfoliata indicated strongest antigenicity/immunogenicity with 13-, 30- and 100-kDa proteins, involving a thioredoxin, heat-shock chaperone 90 (Hsp90), dynein light chain component (DYNLL), tubulin-specific chaperone A (TBCA) and signaling pathway modulators (14-3-3 and Sj-Ts4). This is among the first studies identifying new diagnostic targets and A. perfoliata antigens eliciting a IgG-response in horses.

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Section: Discussionmentioning

confidence: 99%

Label-free quantitative proteomics and immunoblotting identifies immunoreactive and other excretory-secretory (E/S) proteins of Anoplocephala perfoliata

et al. 2022

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“…Derivate from the standard WB according to Tsang et al, the recombinant T14 and T18 antigens have been previously evaluated in the literature (Greene 2000;Assana 2007). Recombinant proteins R93, R914 and R915 have been selected by Nativel et al from T. solium cyst uid (Nativel 2016). All antigens were produced and puri ed by the Recombinant Protein Platform of the Institut Pasteur in Paris.…”

Section: Antigens Preparationmentioning

confidence: 99%

“…Facing the need of an easy to perform method for the discard of suspected animal from the meat chain, new antigens must be identi ed to develop a lateral ow test. During previous studies, antigens from the liquid of the cyst were selected using serum from T. solium infected pigs and then identi ed and cloned (Nativel 2016). As a preliminary step for the selection of new antigens usable at the farm gate, this study was conducted to evaluate the diagnostic value of ve of these recombinant antigens in comparison with IETB according to Tsang et al (1989).…”

Section: Introductionmentioning

confidence: 99%

Porcine cysticercosis: first evaluation of antigens from the liquid of cyst for the development of a serological test for Livestock

Kouadio,

Razafiarimanga,

Randriamparany

et al. 2024

Preprint

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Background: In low-income countries, rapid discard of cysticercosis suspected pigs from the meat chain, is a key step to preserve customers. Unfortunately, in most place only rapid visual inspection of carcasses is practiced. Despite their fluctuant specificity serological tests performed in living animals before entry at slaughterhouse, could be a better and easier approach to promote. However, recombinant antigens must be developed and evaluated to improve accessibility of serological diagnosis. In this study, five recombinant antigens have been expressed and evaluated in Elisa in comparison with electro-immune transfer blot (EITB). Results Antigens were expressed in a baculovirus-insect cell system, purified by affinity chromatography and evaluated on pig serum. Sensitivity, specificity, concordance, and positive likelihood ratio of each recombinant antigen were calculated in reference to EITB. Sensitivityvalues ranged from 43.9% (34.5 - 53.8) to 88.8% (80.8 - 93.7). Specificities ranged from 37.5% (28.5 - 47.5) to 65.6% (55.7 - 74.3). To improve performance of the tests, combination of antigens was used rising sensitivity between 90.8% and 94.9%. Compared to visual examination of carcasses as performed in slaughterhouses these combinations only missed 1% to 5% of infection. Specificities were lower but at the same level as the native antigen ELISA. Conclusion: These recombinant antigens have sensitivity and specificity in the same range as EITB and can be now evaluated against the necropsy as gold standard. Using such tests, cysticercosis suspected animals could be treated with oxendazole before entering the market chain to improve the management of animals at the farm level.

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“…Production of recombinant specific glycoprotein (GP8V2, GP14 and GP18) was implemented to improve the performance of the available tests but also to design immunochromatographic rapid tests [32]. A bio-guided identification of new proteins in cysticercus fluid enabled the Pasteur Institute team to identify 18 new proteins of interest, nine were selected for further development [33].…”

Section: Ongoing Developments Of Diagnostic Tools Of Cysticercosis Inmentioning

confidence: 99%

Cysticercosis in Madagascar

Carod

Dorny

2020

J Infect Dev Ctries

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Introduction: Cysticercosis (CC) is the most common parasitic disease of the central nervous system. It is endemic in most developing countries where pigs are raised and consumed. An overview of all available data of this parasite in Madagascar is lacking. Methodology: We conducted a literature review, collecting information on published and available literature about cysticercosis in Madagascar between January 1st, 1990 and June 30th, 2020. Results: Out of 858 publications; 61 were included, issued from peer-review indexed journals, non-indexed journals, books, Ministry reports and press releases. In Madagascar, porcine cysticercosis has been reported since 1901; human cysticercosis is highly prevalent with an overall estimated seroprevalence between 7 and 21%. Serological analysis is based on Enzyme-Linked Immunosorbent Assay (ELISA) and Enzyme-linked immunoelectrotransfer blot techniques (EITB) for confirmative testing. Neurocysticercosis (NCC) is the most common pattern of cysticercosis in Madagascar and it is reponsible for pediatric morbidity causing more than 50% of epilepsy cases. Though CT-Scan is now available and tends to be considered the gold standard for NCC diagnosis, it remains unaffordable for most Malagasy patients and implies the proposal of a diagnostic algorithm for physicians. Conclusions: Our review has revealed that human taeniasis and bovine cysticercosis is a considerable burden in Madagascar. A national control program has been developed aiming to decrease the seroprevalence rate from 16 to 10% in 2015. The aim of the country is now to implement a CC control and elimination program. Meanwhile, some massive cysticercosis screenings have been conducted in the capital Antananarivo to drive people's attention on this widespread infection.

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Bio-guided identification of proteins for the diagnosis of cysticercosis in swine

Cited by 5 publications

References 5 publications

Label-free quantitative proteomics and immunoblotting identifies immunoreactive and other excretory-secretory (E/S) proteins of Anoplocephala perfoliata

Label-free quantitative proteomics and immunoblotting identifies immunoreactive and other excretory-secretory (E/S) proteins of Anoplocephala perfoliata

Porcine cysticercosis: first evaluation of antigens from the liquid of cyst for the development of a serological test for Livestock

Cysticercosis in Madagascar

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