Bio-functionalized surface designs necessary for applications in regenerative medicine

Abstract

Modification of cell culture substrates and scaffolds with bioactive molecules is frequently used to enhance and/or regulate cellular functions and metabolism during culture. These biomaterials have contributed to an advanced understanding of cell biology and materials designs in biomedical applications. In contrast, we have proposed a new technology ----for realization of tissue/organ reconstruction with structural and functional regeneration. Using cell sheet engineering, confluently cultured cells are harvested as viable, contiguous cell monolayers applied for fabrication of three-dimensional biomimetic tissues or cell sheet-utilized therapies. We have recently introduced bio-functionalization of thermoresponsive surfaces with cell adhesive peptides, e.g., RGDS, and/or cell growth factors, e.g., insulin (INS), for rapid fabrication of cell sheets. Surface-immobilized RGDS peptides promote initial cell adhesion, while INS immobilization accelerates proliferation of adherent cells. More pronounced and synergistic influences on cell growth are observed on RGDS-INS co-immobilized thermoresponsive surfaces even under the serum-free culture conditions. Here, we briefly review the current findings of our bio-functionalized thermoresponsive surfaces for rapid, effective cell sheet fabrication and non-invasive harvest as tissue monolayers for further applications in regenerative medicine.Rec. 4/7/20064/7/ , Acc.5/23/2006

Covalently immobilized biosignal molecule materials for tissue engineering

“…They observed growth enhancement by immobilized insulin and harvested the cells by lowering the temperature. Coimmobilization with insulin and RGDS peptide was reported by Hatakeyama et al [45][46][47] recently.…”

“…Biological or physico-chemical enhancement of cell adhesion increased the mitogenic effect of immobilized growth factors. [33][34][35][36][37][38][39][40]45,46 For biological enhancement of cell adhesion, cell adhesion factors including collagen, fibronectin, gelatin, and the core RGDS peptide were co-immobilized. On the co-immobilized surface both adhesion and growth of cells were remarkably enhanced.…”

Macrophages behavior on different NIPAm‐based thermoresponsive substrates

“…Thermoresponsive materials and surfaces are powerful tools for creating tissue‐like constructs that imitate native tissue geometry and mimic its spatial cellular organization . A lot of attention is focussed on the rational design of biomaterials for cell culture and for further utilization in tissue reconstructions . The interest in thermoresponsive polymers has steadily grown over decades, and a great deal of work has been dedicated to developing temperature sensitive macromolecules that can be crafted into new smart materials .…”

“…2 A lot of attention is focussed on the rational design of biomaterials for cell culture and for further utilization in tissue reconstructions. 3 The interest in thermoresponsive polymers has steadily grown over decades, and a great deal of work has been dedicated to developing temperature sensitive macromolecules that can be crafted into new smart materials. 4 A recently emerging area is the development of thermoresponsive polymers to cultivate and harvest cells which are able to prevent any animal interference.…”