Binding of pulmonary surfactant proteins to carbon nanotubes; potential for damage to lung immune defense mechanisms

Salvador-Morales, Carolina; Paul, Thierry; Flahaut, Emmanuel; Vénien-Bryan, Catherine; Vlandas, Alexis; Green, Malcolm L. H.; Sim, Robert B.

doi:10.1016/j.carbon.2006.10.011

Cited by 106 publications

(74 citation statements)

References 28 publications

(45 reference statements)

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“…As pBALF is not Ca 2+ -free, an ion-mediated adsorption to the organic groups of the two particles via the CRD domain of SP-A may be possible, similarly as has been suspected by Salvador-Morales and Co-workers for accidental organic modifications of carbon nanotubes [28]. black is nearly total, the possibility of not only protein-particle, but also protein-protein interaction is given.…”

Section: Particle Propertiesmentioning

confidence: 85%

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Interaction of metal oxide nanoparticles with lung surfactant protein A

Schulze

Schaefer

Ruge

et al. 2011

European Journal of Pharmaceutics and Biopharmaceutics

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Section: Particle Propertiesmentioning

confidence: 85%

“…A major biological role of SP-A is to bind to inhaled particulate matter (e.g. microorganisms, dust, etc) to enhance their phagocytic clearance by macrophages [28,48,49]. In this way, SP-A plays an important role in limiting pulmonary infection, lung allergy and inflammation [50].…”

Section: Investigation Of Deagglomeration Capacity Of Pbalfmentioning

confidence: 99%

Interaction of metal oxide nanoparticles with lung surfactant protein A

Schulze

Schaefer

Ruge

et al. 2011

European Journal of Pharmaceutics and Biopharmaceutics

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“…53 It has also been demonstrated that chronic exposure to CNTs results in a sequestration of surfactant proteins A and D ͑SP-A and SP-D͒ and collectins, which are known to play a relevant defensive role against infections within the lung. 54 As a consequence, a relevant reduction in immune defense is induced due to both macrophages impairment and direct binding of surfactant proteins. The extent of these effects is likely to be driven by morphology and dimensions of CNTs.…”

Section: Biological Bases Of Cnt Toxicitymentioning

confidence: 99%

Carbon nanotubes and pleural damage: Perspectives of nanosafety in the light of asbestos experience

Stella¹

2011

Biointerphases

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“…For example, CNTs could bind with pulmonary surfactant proteins A and D and then lead to susceptibility of lung infection and emphysema in mice (29). When the functionalized CNTs were bound to the α-chymotrypsin, the complex could inhibit enzymatic activity (30).…”

mentioning

confidence: 99%

Binding of blood proteins to carbon nanotubes reduces cytotoxicity

Zhao

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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766

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With the potential wide uses of nanoparticles such as carbon nanotubes in biomedical applications, and the growing concerns of nanotoxicity of these engineered nanoparticles, the importance of nanoparticle-protein interactions cannot be stressed enough. In this study, we use both experimental and theoretical approaches, including atomic force microscope images, fluorescence spectroscopy, CD, SDS-PAGE, and molecular dynamics simulations, to investigate the interactions of single-wall carbon nanotubes (SWCNTs) with human serum proteins, and find a competitive binding of these proteins with different adsorption capacity and packing modes. The π-π stacking interactions between SWCNTs and aromatic residues (Trp, Phe, Tyr) are found to play a critical role in determining their adsorption capacity. Additional cellular cytotoxicity assays, with human acute monocytic leukemia cell line and human umbilical vein endothelial cells, reveal that the competitive bindings of blood proteins on the SWCNT surface can greatly alter their cellular interaction pathways and result in much reduced cytotoxicity for these protein-coated SWCNTs, according to their respective adsorption capacity. These findings have shed light toward the design of safe carbon nanotube nanomaterials by comprehensive preconsideration of their interactions with human serum proteins.competitive adsorption | nanoparticle-protein corona | hydrophobic interactions | conformational flexibility C arbon nanotubes (CNTs) have a broad range of potential applications, including nanoelectronics (1), biosensors (2), biomolecular recognition devices and molecular transporters (3, 4), and cancer therapy and diagnoses (5-7), because of their extraordinary mechanical, electronic, optical, geometric, and biological properties. Both the pharmacological (8, 9) and toxicological (10-15) profiles of CNTs have attracted much attention in recent years. Nanomedicine provides a great potential in fighting many diseases but a global effort is much needed to safely translate laboratory innovations into clinic trials. Seven grand challenges have been proposed for this endeavor (16). CNTbased multimodality imaging and therapy have been proposed for a variety of medical applications, such as carriers for chemotherapeutic drugs (17, 18), Raman detection and imaging (19,20), photoacoustic imaging, and photothermal therapy of cancers (6, 21). Meanwhile, of equal importance there is the emerging field of nanotoxicology, which aims to address the biosafety of these engineered nanoparticles, in particular, how these nanoparticles interact with human proteins in vivo (22)(23)(24)(25).Many studies have been performed on the biological effects of carbon nanotubes, and the interactions between proteins and CNTs (nanoparticle-protein corona) are believed to play an important role in the biological effects of CNTs (26-28). For example, CNTs could bind with pulmonary surfactant proteins A and D and then lead to susceptibility of lung infection and emphysema in mice (29). When the functionalized CNTs w...

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Binding of pulmonary surfactant proteins to carbon nanotubes; potential for damage to lung immune defense mechanisms

Abstract: OATAO is an open access repository that collects the work of Toulouse researchers and makes it freely available over the web where possible.

Cited by 106 publications

References 28 publications

Interaction of metal oxide nanoparticles with lung surfactant protein A

Interaction of metal oxide nanoparticles with lung surfactant protein A

Carbon nanotubes and pleural damage: Perspectives of nanosafety in the light of asbestos experience

Binding of blood proteins to carbon nanotubes reduces cytotoxicity

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