Binding of Bile Salts by Soluble Fibers and Its Effect on Deconjugation of Glycocholate by Lactobacillus acidophilus and Lactobacillus casei.

Abstract

Binding of sodium cholate, sodium taurocholate, and sodium glycocholate by guar gum, soluble oat fiber, xanthan gum, and inulin was studied. All soluble fibers were able to bind sodium cholate, sodium taurocholate, and sodium glycocholate from a mixture of the three, but when tested individually, the fibers bound little or no sodium cholate. In general, the fibers bound higher concentrations of conjugated bile salts than they did free bile salts. Soluble oat fiber, which bound as much or more sodium cholate and sodium glycocholate as did the other 3 fibers, was tested for its effect on deconjugation of glycocholate by Lactobacillus acidophilus and Lactobacillus casei. The presence of the fiber significantly increased (P < 0.05) deconjugation by all cultures.

Environmental Factors Affecting the Adhesion of Probiotics to Rice Fiber Fractions

“…In contrast, deconjugated bile (free bile salt) inhibits probiotics. Previous studies have noticed that free bile has an ability to adhere to soluble fiber [12,46]. However, in the present study in the presence of bile, the microorganisms had a moderate affinity for fiber and not only for soluble fiber but for total and insoluble fiber (Fig.…”

Re-evaluation of the mechanisms of dietary fibre and implications for macronutrient bioaccessibility, digestion and postprandial metabolism

“…Thus, soluble fibre is thought to have an impact on bile salt recycling plus mixing and transport of mixed micelles ( 136 ) . It has been suggested that soluble forms of fibre exert their plasma cholesterol-lowering effect by direct ‘binding’ of polysaccharides to bile salts, although the type of binding involved here is unclear ( 136 , 145 – 147 ) . A more likely explanation may be that there is a temporary entrapment of the bile salts within the viscous network of entangled polysaccharide chains ( 148 – 151 ) .…”