Binding of angiogenesis inhibitor kringle 5 to its specific ligands by frontal affinity chromatography

Bian, Liujiao; Li, Qian; Ji, Xu

doi:10.1016/j.chroma.2015.04.058

Cited by 16 publications

(2 citation statements)

References 47 publications

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“…FAC assay provides valuable information regarding the bioaffinity column and the binding event, such as the dissociation constant (K D ), the number of active sites (n) present in the IMER, and the number of ligands interacting with the target (n L ). Exploring this approach, angiogenesis inhibitor Kringle 5 (Bian et al, 2015), TEM-1 beta-lactamase (Chen et al, 2017), trypsin (Qian et al, 2019), thrombin (Yang et al, 2017) and many other proteins were successfully studied. These parameters give information that allows ranking the ligands regarding their binding affinity to the biomolecule proving to be a useful tool to better understand binding events.…”

Section: Frontal Affinity Chromatographymentioning

confidence: 99%

On-flow enzymatic inhibitor screening: The emerging success of liquid chromatography-based assays

et al. 2022

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Enzymes are targets commonly explored in screening assays aiming to discover new leads in the drug development process. Among the diverse assay models to identify new enzymatic inhibitors, on-flow assays based on liquid chromatography (LC) can be highlighted. In these approaches, the ligand-enzyme interaction can be examined by monitoring the catalytic activity or the affinity/retention. Most applications use the biological target immobilized in solid supports resulting in the acquisition of an immobilized enzymatic reactor (IMER). Coupling IMERs to LC or mass spectrometry (MS) systems allows monitoring enzyme activity online and studying binding events between target and ligands. On-flow screening assays present many advantages for the hit-to-lead process, such as the possibility of system automation, reusability, and high stability. This review covers articles from the last decade that combine the use of varied immobilization methods on different solid supports and several equipment setups in on-flow systems, emphasizing the performance and capacity of recognizing and identifying biologically active compounds in various matrices.

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Section: Frontal Affinity Chromatographymentioning

confidence: 99%

On-flow enzymatic inhibitor screening: The emerging success of liquid chromatography-based assays

et al. 2022

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“…Kringle domain exists extensively in plasminogen and apolipoprotein, veri ed to play a key role in the inhibition of angiogenesis ,especially Kringle (K ). [7][8][9][10][11] Plasminogen Kringe is con rmed to suppress angiogenesis in gastric cancer, hepatocellular carcinoma and corneal neovascularization. [12][13][14] And Don J.…”

Section: Introductionmentioning

confidence: 99%

Exosome-Mediated Delivery of an Anti-Angiogenic Peptide Inhibits Corneal Neovascularization via VDAC1 and Autophagy

Chen

2022

Preprint

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Background: Angiogenesis is a crucial reason for the poor prognosis of corneal diseases. KV11, an undecanoic peptide from Kringle Ⅴ domain derived from apolipoprotein(a) is identified to inhibit angiogenesis significantly. Given the poor permeability and instability of small peptide, we aim to use exosome and its anchoring peptide CP05 to deliver KV11 to alkali-burn cornea tissue and inhibit corneal neovascularization.Methods: In vitro, we tested the effect of EXO-KV11 on inhibition of angiogenesis with the method of tube formation, transwell migration assay and CCK-8. In vivo, we used subconjunctival injection of EXO-KV11 to the alkali-burn cornea-induced corneal neovascularization rat model and fluorescent angiography to directly detect the impact on corneal neovascularization. We also revealed the underlying mechanism by western blot.Results: EXO-KV11 was verified to suppress endothelial cells tube formation, migration and proliferation in vitro by tube formation assay, transwell migration assay and CCK-8 assy. EXO-KV11 was more efficiently delivered to cornea tissue and suppressed more corneal neovascularization in alkali burn-induced corneal neovascularization model. Furthermore, VDAC1 might be potential receptor for KV11 and KV11 stimulated PERK and blocked up autography flux.Conclusions: We used exosomes to delivery KV11 into cells and cornea tissue. We reported that EXO-KV11 could inhibit angiogenesis more than KV11 both in vitro and in vivo. Thus exosome-CP05 drug delivery system has promising prospect in the future.

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Derivation of adsorption capacity and adsorption isotherm by a single adsorbate concentration in liquid–solid system

et al. 2021

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Binding of angiogenesis inhibitor kringle 5 to its specific ligands by frontal affinity chromatography

Cited by 16 publications

References 47 publications

On-flow enzymatic inhibitor screening: The emerging success of liquid chromatography-based assays

On-flow enzymatic inhibitor screening: The emerging success of liquid chromatography-based assays

Exosome-Mediated Delivery of an Anti-Angiogenic Peptide Inhibits Corneal Neovascularization via VDAC1 and Autophagy

Derivation of adsorption capacity and adsorption isotherm by a single adsorbate concentration in liquid–solid system

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