Bifidobacterium animalis subsp lactis HN019 presents antimicrobial potential against periodontopathogens and modulates the immunological response of oral mucosa in periodontitis patients

Abstract

Objective To evaluate the effects of Bifidobacterium animalis subsp. lactis HN019 (HN019) on clinical periodontal parameters (plaque accumulation and gingival bleeding), on immunocompetence of gingival tissues [expression of beta-defensin (BD)-3, toll-like receptor 4 (TLR4), cluster of differentiation(CD)-57 and CD-4], and on immunological properties of saliva (IgA levels) in non-surgical periodontal therapy in generalized chronic periodontitis (GCP) patients. Adhesion to buccal epithelial cells (BEC) and the antimicrobial properties of HN019 were also investigated. Materials and methods Thirty patients were recruited and monitored clinically at baseline (before scaling and root planing-SRP) and after 30 and 90 days. Patients were randomly assigned to Test (SRP +Probiotic, n = 15) or Control (SRP+Placebo, n = 15) group. Probiotic lozenges were used for 30 days. Gingival tissues and saliva were immunologically analyzed. The adhesion of HN019 with or without Porphyromonas gingivalis in BEC and its antimicrobial properties were investigated in in vitro assays. Data were statistically analyzed (p<0.05). Results Test group presented lower plaque index (30 days) and lower marginal gingival bleeding (90 days) when compared with Control group. Higher BD-3, TLR4 and CD-4 expressions were

Pre and Probiotics Involved in the Modulation of Oral Bacterial Species: New Therapeutic Leads in Mental Disorders?

“…Each study that met the inclusion criteria was analyzed to identify prebiotic compounds or probiotic strains and their effects on the oral microbial flora or immunological markers involved in mental disorders. Three distinct effects on oral dysbiosis associated with mental disorders emerged: modulation of the oral microbiome [ 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 ], antimicrobial activity [ 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 ], and modulation of immunological or inflammatory factors [ 43 , 44 , 46 , 56 , 70 , 71 , 72 , 73 , 74 , 75 ].…”

“…Table 3 shows the different probiotic strains investigated alone or in combination in 12 in vitro studies [ 41 , 42 , 43 , 44 , 45 , 46 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 ] and 23 in vivo studies [ 46 , 47 , 48 , 49 , 50 , 51 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 ] including three using animal models [ 46 , 70 , 71 ]. The experiments in patients were performed with small samples ( n = 12 to 108) and a short follow-up period (1 day to 36 weeks).…”

“…Thus, Bifidobacterium animalis subsp. lactis HN019 used for 30 days (lozanges) reduce the adhesion of P. gingivalis to buccal epithelial cells [ 47 ]. Short-term consumption (1 day) of milk fermented with probiotic strains ( Streptococcus thermophilus , Lactobacillus delbrueckii , and Lactobacillus paracasei ) supplemented with vitamin B6 and vitamin D increases the overall diversity of the oral cavity microbiome with an increase in the genera Steptococcus and Actinomyces.…”

β-Galactosidase-Producing Isolates in Mucoromycota: Screening, Enzyme Production, and Applications for Functional Oligosaccharide Synthesis

“…The tested L. casei , L. acidophilus , B. animalis subsp. lactis and S. boulardii probiotic microorganisms possess many properties beneficial for human health [ 69 , 70 , 71 ], including the action against pathogens [ 72 , 73 , 74 ]. The probiotic microorganisms responded to the oligosaccharide-enriched mixtures differently, however, in most cases, considerable growth-promoting effect was identified.…”

Host Modulation and Treatment of Periodontal Disease

“…In addition, studies with small sample sizes testing unrelated microorganisms with different vehicles of administration were grouped in the scope of meta-analyses, thereby skewing results (Appendix). Of note, while regulation of cytokines such as IL-10, IL-1β, and IL-8 and host protective proteins such as human beta defensin 3, TLR4, and CD-4 is reported, most clinical studies attribute additional improvement to decreases in periopathogens rather than host modulation (Invernici et al 2018; Invernici et al 2020).…”