2018
DOI: 10.1186/s12964-018-0307-1
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: BackgroundHuman pluripotent stem cells (PSCs) open new windows for basic research and regenerative medicine due to their remarkable properties, i.e. their ability to self-renew indefinitely and being pluripotent. There are different, conflicting data related to the role of basic fibroblast growth factor (bFGF) in intracellular signal transduction and the regulation of pluripotency of PSCs. Here, we investigated the effect of bFGF and its downstream pathways in pluripotent vs. differentiated human induced (hi) … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

2
26
0
1

Year Published

2019
2019
2023
2023

Publication Types

Select...
8

Relationship

0
8

Authors

Journals

citations
Cited by 36 publications
(29 citation statements)
references
References 52 publications
2
26
0
1
Order By: Relevance
“…The induction of active pErk1/2 was further connected to reduced NANOG gene levels [37]. In line with these data from iPSCs and hPSCs [36, 37, 54], we show an enhanced expression of stemness/self-renewal-related genes in obese ASCs after inhibition of either Aurora A or Erk1/2. Both inhibitors lead to the activation of the STAT3 and PI3K/AKT pathways, which phosphorylates its downstream target GSK3β on serine 9 [55].…”
Section: Discussionsupporting
confidence: 80%
See 1 more Smart Citation
“…The induction of active pErk1/2 was further connected to reduced NANOG gene levels [37]. In line with these data from iPSCs and hPSCs [36, 37, 54], we show an enhanced expression of stemness/self-renewal-related genes in obese ASCs after inhibition of either Aurora A or Erk1/2. Both inhibitors lead to the activation of the STAT3 and PI3K/AKT pathways, which phosphorylates its downstream target GSK3β on serine 9 [55].…”
Section: Discussionsupporting
confidence: 80%
“…Our observation showing increased stem cell/self-renewal genes after the treatment with MLN is supported by a study where the inhibition of Aurora A enhances the reprogramming efficiency of induced pluripotent stem cells (iPSCs) linked to increased gene levels of NANOG , TET1 , and ERas , mediated by GSK3β inactivation [35], which is necessary to promote iPSC generation [53]. Moreover, human pluripotent stem cells (hPSCs) require the PI3K/AKT activity to maintain the self-renewal state by suppression of the MAPK/Erk and canonical Wnt pathways [36, 37, 54]. The induction of active pErk1/2 was further connected to reduced NANOG gene levels [37].…”
Section: Discussionmentioning
confidence: 99%
“…PRMT8/PI3K/AKT axis maintains pluripotency of hESC as well as mesodermal differentiation via the regulation of Sox2 (Jeong et al, 2017). In a recent study, Haghighi et al (2018) showed that among the downstream mediators of FGF2 signaling pathways, the MAPK pathway plays a pivotal role in maintaining the pluripotency of hiPSCs. They demonstrated that following withdrawal of FGF2, the activity of NRAS-RAF-MEK-ERK declined, while the AKT signaling pathway as one of the downstream of the FGF2 remained unchanged (Haghighi et al, 2018).…”
Section: Fgf2mentioning
confidence: 99%
“…In a recent study, Haghighi et al (2018) showed that among the downstream mediators of FGF2 signaling pathways, the MAPK pathway plays a pivotal role in maintaining the pluripotency of hiPSCs. They demonstrated that following withdrawal of FGF2, the activity of NRAS-RAF-MEK-ERK declined, while the AKT signaling pathway as one of the downstream of the FGF2 remained unchanged (Haghighi et al, 2018). In another study, it has been shown that the C5a complement member supported the pluripotency of hESC after removal of FGF2 through the ERK1/2 signaling pathway (Hawksworth et al, 2014).…”
Section: Fgf2mentioning
confidence: 99%
“…It is well known that FGF2 (or basic FGF)/Erk signaling in hESCs maintains the primed pluripotent state and blocks neuronal, trophectoderm, and primitive endoderm differentiation both directly and indirectly via activin/nodal induction [24, 31]. In addition, extrinsic FGF2 signal can directly regulate Nanog expression [32] and stimulate the Ras paralog, NRas, which is linked to the MAPK pathway [33], thereby sustaining human PSC (hPSC) pluripotency. Interestingly, Erk1/2 inhibition establishes the naïve ground state of adherent hPSCs [34] but leads to a loss of the pluripotent phenotype in the suspension culture of hPSCs, suggesting that Erk is involved in a different mechanism for the suspension environment [35].…”
Section: Kinase Signaling Pathways In Pluripotency and Self-renewalmentioning
confidence: 99%