Beta vulgaris L. (Beetroot) Methanolic Extract Prevents Hepatic Steatosis and Liver Damage in T2DM Rats by Hypoglycemic, Insulin-Sensitizing, Antioxidant Effects, and Upregulation of PPARα

Abstract

The present study examined if methanolic beetroot extract (BE) could prevent dyslipidemia and hepatic steatosis and damage in a type-2 diabetes mellitus (T2DM) rat model and studied some mechanisms of action. T2DM was induced in adult male Wistar rats by a low single dose of streptozotocin (STZ) (35 mg/kg, i.p) and a high-fat diet (HFD) feeding for 5 weeks. Control or T2DM rats then continued on standard or HFDs for another 12 weeks and were treated with the vehicle or BE (250 or 500 mg/kg). BE, at both doses, significantly improved liver structure and reduced hepatic lipid accumulation in the livers of T2DM rats. They also reduced body weight gain, serum glucose, insulin levels, serum and hepatic levels of cholesterol, triglycerides, free fatty acids, and serum levels of low-density lipoproteins in T2DM rats. In concomitant, they significantly reduced serum levels of aspartate and alanine aminotransferases, hepatic levels of malondialdehyde, tumor-necrosis factor-α, interleukin-6, and mRNA of Bax, cleaved caspase-3, and SREBP1/2. However, both doses of BE significantly increased hepatic levels of total glutathione, superoxide dismutase, and mRNA levels of Bcl2 and PPARα in the livers of both the control and T2DM rats. All of these effects were dose-dependent and more profound with doses of 500 mg/kg. In conclusion, chronic feeding of BE to STZ/HFD-induced T2DM in rats prevents hepatic steatosis and liver damage by its hypoglycemic and insulin-sensitizing effects and its ability to upregulate antioxidants and PPARα.

Beta vulgaris var. cicla methanol extract prevents the formation of advanced glycation end products and protein oxidation against -glucose, and -fructose-induced protein glycation in vitro

“…resulting in scientific investigations focused on the development of novel agents with anti-glycation effects with the ability to prevent and alleviate diabetes complications. Due to the traditional usage of chard as antidiabetic and supported by in vivo experiments [7]. we studied CH extract as a potential agent against BSA glycation.…”

“…flavescens has been used in traditional medicine for its antibacterial and hypoglycemic properties (5). Contains phytochemicals that have beneficial activities including anti-inflammatory, antioxidant, and antiproliferative effects (6) The antidiabetic potential of B. vulgaris leaves was reported in diabetic Wistar rats induced with streptozotocin (STZ) and a high-fat diet (HFD) demonstrated that prevents it liver damage and hepatic steatosis by its insulin-sensitizing, and hypoglycemic properties and their capacity to upregulate antioxidants and PPARα (7). According to the literature, there is no report of in vitro anti-glycation activity of B. vulgaris leaves thus, our investigation aimed to determine the anti-glycation effect of the leaves extracts of B. vulgaris by using a battery of invitro assays.…”

Hypolipidemic Effects of Beetroot Juice in SHR-CRP and HHTg Rat Models of Metabolic Syndrome: Analysis of Hepatic Proteome

“…It has been reported that treatment of Wistar rats with type 2 diabetes induced by STZ with methanolic beetroot extract and a high-fat diet reduced serum and hepatic levels of cholesterol, triglycerides, and free fatty acids, which was associated with downregulated transcription of SREBP1/2 and stimulation of PPARα [ 55 ]. Congruent with these results, we observed that the hypolipidemic effects of beetroot in SHR-CRP rats were associated with increased fatty acid oxidation in the skeletal muscle.…”

A Functional Orange Juice Fortified with Beetroot By-Products Attenuates Hyperlipidemia and Obesity Induced by A High-Fat Diet

“…The obtained result agreed with that of Lee et al [37], who observed that involving lyophiliyzed beetroot leaves in rats receiving a high cholesterol diet reduced the gained weight significantly compared to the control. Similarly, Al-Harbi et al [38] noticed that beetroot stem extract reduced the weight in diabetic rats fed with a high-fat diet.…”